Experiment: McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt MMP_RS01235 and MMP_RS01240 are separated by 15 nucleotides MMP_RS01240 and MMP_RS01245 are separated by 131 nucleotides MMP_RS01245 and MMP_RS01250 are separated by 230 nucleotides
MMP_RS01235: MMP_RS01235 - iron-containing alcohol dehydrogenase, at 232,456 to 233,460
_RS01235
MMP_RS01240: MMP_RS01240 - 7-cyano-7-deazaguanine synthase QueC, at 233,476 to 234,177
_RS01240
MMP_RS01245: MMP_RS01245 - anaerobic ribonucleoside-triphosphate reductase, at 234,309 to 236,621
_RS01245
MMP_RS01250: MMP_RS01250 - tRNA (guanine(26)-N(2))-dimethyltransferase, at 236,852 to 237,973
_RS01250
Position (kb) 234
235
236
237 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2
3 at 233.312 kb on - strand at 233.340 kb on + strand at 234.087 kb on - strand, within MMP_RS01240 at 234.094 kb on + strand, within MMP_RS01240 at 234.156 kb on - strand at 234.214 kb on + strand at 234.285 kb on + strand at 234.333 kb on + strand at 234.509 kb on + strand at 235.725 kb on - strand at 236.434 kb on + strand at 236.493 kb on - strand at 236.602 kb on - strand at 236.703 kb on + strand at 236.717 kb on - strand at 236.730 kb on + strand at 236.732 kb on + strand at 236.747 kb on + strand at 236.747 kb on + strand at 236.752 kb on + strand at 236.752 kb on + strand at 236.756 kb on + strand at 236.756 kb on + strand at 236.764 kb on - strand at 236.764 kb on - strand at 236.766 kb on - strand at 236.766 kb on - strand at 236.766 kb on - strand at 236.766 kb on - strand at 236.767 kb on + strand at 236.767 kb on + strand at 236.768 kb on - strand at 236.768 kb on - strand at 236.769 kb on + strand at 236.769 kb on + strand at 236.805 kb on + strand at 236.809 kb on + strand at 236.860 kb on + strand at 236.863 kb on - strand at 236.920 kb on - strand at 237.222 kb on - strand, within MMP_RS01250 at 237.343 kb on + strand, within MMP_RS01250 at 237.572 kb on - strand, within MMP_RS01250 at 237.605 kb on + strand, within MMP_RS01250
Per-strain Table
Position Strand Gene LocusTag Fraction McCas_H2_complete 2,2-dibromo-2-cyanoacetamide 0.0333333333333333 mM remove 233,312 - +3.3 233,340 + -3.0 234,087 - MMP_RS01240 0.87 +2.9 234,094 + MMP_RS01240 0.88 -3.5 234,156 - -1.4 234,214 + -4.8 234,285 + +1.2 234,333 + +1.0 234,509 + -3.3 235,725 - -3.4 236,434 + -1.9 236,493 - -3.3 236,602 - -1.2 236,703 + -2.2 236,717 - -4.1 236,730 + -1.0 236,732 + -0.7 236,747 + -5.7 236,747 + -3.9 236,752 + -1.1 236,752 + -2.8 236,756 + +0.3 236,756 + -0.2 236,764 - -2.8 236,764 - +0.4 236,766 - -3.1 236,766 - -1.1 236,766 - -1.9 236,766 - -0.6 236,767 + -5.3 236,767 + -3.9 236,768 - +1.6 236,768 - -3.7 236,769 + +3.4 236,769 + -0.6 236,805 + -0.3 236,809 + -2.6 236,860 + -5.8 236,863 - -1.0 236,920 - +0.1 237,222 - MMP_RS01250 0.33 +0.4 237,343 + MMP_RS01250 0.44 +1.0 237,572 - MMP_RS01250 0.64 +1.2 237,605 + MMP_RS01250 0.67 +0.8
Or see this region's nucleotide sequence