Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2020 and DVU2021 overlap by 4 nucleotides DVU2021 and DVU2022 overlap by 1 nucleotides DVU2022 and DVU2023 are separated by 4 nucleotides DVU2023 and DVU2024 overlap by 4 nucleotides
DVU2020: DVU2020 - conserved hypothetical protein (TIGR), at 2,098,260 to 2,100,758
DVU2020
DVU2021: DVU2021 - hypothetical protein (TIGR), at 2,100,755 to 2,101,222
DVU2021
DVU2022: DVU2022 - conserved hypothetical protein (TIGR), at 2,101,222 to 2,104,698
DVU2022
DVU2023: DVU2023 - hypothetical protein (TIGR), at 2,104,703 to 2,105,242
DVU2023
DVU2024: DVU2024 - conserved hypothetical protein (TIGR), at 2,105,239 to 2,106,303
DVU2024
Position (kb)
2101
2102
2103
2104
2105 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2100.414 kb on + strand, within DVU2020 at 2100.528 kb on - strand at 2100.558 kb on - strand at 2100.899 kb on - strand, within DVU2021 at 2101.154 kb on - strand, within DVU2021 at 2101.330 kb on - strand at 2101.342 kb on - strand at 2101.419 kb on - strand at 2101.484 kb on + strand at 2101.505 kb on + strand at 2101.547 kb on + strand at 2101.547 kb on + strand at 2101.555 kb on - strand at 2101.555 kb on - strand at 2101.572 kb on + strand, within DVU2022 at 2101.639 kb on - strand, within DVU2022 at 2101.662 kb on + strand, within DVU2022 at 2101.679 kb on + strand, within DVU2022 at 2101.687 kb on - strand, within DVU2022 at 2101.687 kb on - strand, within DVU2022 at 2101.816 kb on - strand, within DVU2022 at 2101.873 kb on - strand, within DVU2022 at 2101.897 kb on - strand, within DVU2022 at 2101.936 kb on + strand, within DVU2022 at 2102.005 kb on - strand, within DVU2022 at 2102.033 kb on - strand, within DVU2022 at 2102.038 kb on + strand, within DVU2022 at 2102.038 kb on + strand, within DVU2022 at 2102.145 kb on + strand, within DVU2022 at 2102.195 kb on - strand, within DVU2022 at 2102.522 kb on - strand, within DVU2022 at 2102.911 kb on - strand, within DVU2022 at 2103.450 kb on + strand, within DVU2022 at 2103.495 kb on + strand, within DVU2022 at 2103.506 kb on + strand, within DVU2022 at 2103.509 kb on - strand, within DVU2022 at 2103.514 kb on - strand, within DVU2022 at 2103.529 kb on - strand, within DVU2022 at 2103.646 kb on + strand, within DVU2022 at 2103.650 kb on + strand, within DVU2022 at 2103.655 kb on + strand, within DVU2022 at 2103.660 kb on + strand, within DVU2022 at 2103.719 kb on - strand, within DVU2022 at 2103.748 kb on + strand, within DVU2022 at 2103.773 kb on + strand, within DVU2022 at 2103.773 kb on + strand, within DVU2022 at 2103.781 kb on - strand, within DVU2022 at 2103.781 kb on - strand, within DVU2022 at 2103.789 kb on + strand, within DVU2022 at 2103.950 kb on - strand, within DVU2022 at 2103.995 kb on + strand, within DVU2022 at 2104.002 kb on + strand, within DVU2022 at 2104.216 kb on - strand, within DVU2022 at 2104.289 kb on + strand, within DVU2022 at 2104.289 kb on + strand, within DVU2022 at 2104.330 kb on - strand, within DVU2022 at 2104.388 kb on + strand at 2104.422 kb on + strand at 2104.422 kb on + strand at 2104.470 kb on - strand at 2104.583 kb on + strand at 2104.603 kb on + strand at 2104.727 kb on + strand at 2104.777 kb on + strand, within DVU2023 at 2104.799 kb on + strand, within DVU2023 at 2104.805 kb on + strand, within DVU2023 at 2104.805 kb on + strand, within DVU2023 at 2104.805 kb on + strand, within DVU2023 at 2104.805 kb on + strand, within DVU2023 at 2104.813 kb on - strand, within DVU2023 at 2104.813 kb on - strand, within DVU2023 at 2105.073 kb on + strand, within DVU2023 at 2105.083 kb on - strand, within DVU2023 at 2105.197 kb on - strand at 2105.197 kb on - strand at 2105.405 kb on + strand, within DVU2024 at 2105.405 kb on + strand, within DVU2024 at 2105.455 kb on - strand, within DVU2024 at 2105.555 kb on + strand, within DVU2024 at 2105.555 kb on + strand, within DVU2024 at 2105.563 kb on - strand, within DVU2024
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,100,414 + DVU2020 0.86 +0.1 2,100,528 - +0.1 2,100,558 - +0.9 2,100,899 - DVU2021 0.31 -0.1 2,101,154 - DVU2021 0.85 +0.1 2,101,330 - +1.7 2,101,342 - +0.3 2,101,419 - +0.4 2,101,484 + -0.2 2,101,505 + -0.5 2,101,547 + -0.7 2,101,547 + +1.0 2,101,555 - +0.1 2,101,555 - -0.6 2,101,572 + DVU2022 0.10 +0.2 2,101,639 - DVU2022 0.12 -0.7 2,101,662 + DVU2022 0.13 +0.1 2,101,679 + DVU2022 0.13 -0.1 2,101,687 - DVU2022 0.13 +0.9 2,101,687 - DVU2022 0.13 -0.7 2,101,816 - DVU2022 0.17 +0.3 2,101,873 - DVU2022 0.19 +0.1 2,101,897 - DVU2022 0.19 -0.2 2,101,936 + DVU2022 0.21 +0.4 2,102,005 - DVU2022 0.23 +1.5 2,102,033 - DVU2022 0.23 +0.2 2,102,038 + DVU2022 0.23 +0.1 2,102,038 + DVU2022 0.23 +0.1 2,102,145 + DVU2022 0.27 -0.4 2,102,195 - DVU2022 0.28 +0.2 2,102,522 - DVU2022 0.37 -1.2 2,102,911 - DVU2022 0.49 -1.2 2,103,450 + DVU2022 0.64 +0.1 2,103,495 + DVU2022 0.65 +0.9 2,103,506 + DVU2022 0.66 +1.0 2,103,509 - DVU2022 0.66 -1.9 2,103,514 - DVU2022 0.66 +0.2 2,103,529 - DVU2022 0.66 +0.5 2,103,646 + DVU2022 0.70 +0.1 2,103,650 + DVU2022 0.70 +0.2 2,103,655 + DVU2022 0.70 +1.7 2,103,660 + DVU2022 0.70 -0.9 2,103,719 - DVU2022 0.72 +0.4 2,103,748 + DVU2022 0.73 +1.1 2,103,773 + DVU2022 0.73 -0.0 2,103,773 + DVU2022 0.73 +0.3 2,103,781 - DVU2022 0.74 +0.1 2,103,781 - DVU2022 0.74 +0.2 2,103,789 + DVU2022 0.74 -0.4 2,103,950 - DVU2022 0.78 -0.0 2,103,995 + DVU2022 0.80 +0.1 2,104,002 + DVU2022 0.80 +3.2 2,104,216 - DVU2022 0.86 -0.6 2,104,289 + DVU2022 0.88 +1.0 2,104,289 + DVU2022 0.88 +2.3 2,104,330 - DVU2022 0.89 +0.2 2,104,388 + +0.4 2,104,422 + +0.2 2,104,422 + +0.0 2,104,470 - -0.0 2,104,583 + +0.2 2,104,603 + +0.7 2,104,727 + -0.6 2,104,777 + DVU2023 0.14 -0.4 2,104,799 + DVU2023 0.18 +0.4 2,104,805 + DVU2023 0.19 -0.1 2,104,805 + DVU2023 0.19 -0.4 2,104,805 + DVU2023 0.19 +0.1 2,104,805 + DVU2023 0.19 +0.8 2,104,813 - DVU2023 0.20 +0.3 2,104,813 - DVU2023 0.20 -2.3 2,105,073 + DVU2023 0.69 -0.4 2,105,083 - DVU2023 0.70 +1.1 2,105,197 - -0.2 2,105,197 - +1.6 2,105,405 + DVU2024 0.16 +1.4 2,105,405 + DVU2024 0.16 -0.1 2,105,455 - DVU2024 0.20 -0.1 2,105,555 + DVU2024 0.30 -0.5 2,105,555 + DVU2024 0.30 -2.5 2,105,563 - DVU2024 0.30 +0.5
Or see this region's nucleotide sequence