Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0358 and DVU0359 are separated by 282 nucleotides DVU0359 and ilvB-1 are separated by 696 nucleotides
DVU0358: DVU0358 - hypothetical protein (TIGR), at 407,342 to 408,085
DVU0358
DVU0359: DVU0359 - HesB-like domain (TIGR), at 408,368 to 408,694
DVU0359
DVU0360: ilvB-1 - acetolactate synthase, large subunit, biosynthetic type (TIGR), at 409,391 to 411,082
ilvB-1
Position (kb)
408
409 Strain fitness (log2 ratio)
-1
0
1
2 at 407.502 kb on - strand, within DVU0358 at 407.595 kb on - strand, within DVU0358 at 407.595 kb on - strand, within DVU0358 at 407.711 kb on - strand, within DVU0358 at 407.758 kb on - strand, within DVU0358 at 407.881 kb on + strand, within DVU0358 at 407.889 kb on - strand, within DVU0358 at 407.923 kb on - strand, within DVU0358 at 407.928 kb on - strand, within DVU0358 at 407.933 kb on - strand, within DVU0358 at 407.985 kb on + strand, within DVU0358 at 407.998 kb on - strand, within DVU0358 at 408.109 kb on + strand at 408.116 kb on + strand at 408.124 kb on - strand at 408.142 kb on - strand at 408.165 kb on - strand at 408.313 kb on + strand at 408.379 kb on - strand at 408.482 kb on + strand, within DVU0359 at 408.514 kb on + strand, within DVU0359 at 408.551 kb on + strand, within DVU0359 at 408.628 kb on - strand, within DVU0359 at 408.846 kb on - strand at 408.934 kb on + strand at 408.942 kb on - strand at 408.975 kb on + strand at 409.019 kb on - strand at 409.021 kb on + strand at 409.022 kb on + strand at 409.071 kb on + strand at 409.116 kb on - strand at 409.128 kb on - strand at 409.148 kb on + strand at 409.338 kb on + strand at 409.345 kb on - strand at 409.345 kb on - strand at 409.346 kb on - strand at 409.436 kb on + strand at 409.486 kb on + strand at 409.526 kb on + strand at 409.534 kb on - strand at 409.536 kb on + strand at 409.536 kb on + strand at 409.544 kb on - strand at 409.544 kb on - strand at 409.557 kb on + strand at 409.576 kb on - strand, within ilvB-1 at 409.620 kb on - strand, within ilvB-1
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 407,502 - DVU0358 0.22 -0.0 407,595 - DVU0358 0.34 +0.2 407,595 - DVU0358 0.34 -0.5 407,711 - DVU0358 0.50 -0.3 407,758 - DVU0358 0.56 -0.5 407,881 + DVU0358 0.72 -1.6 407,889 - DVU0358 0.74 -0.2 407,923 - DVU0358 0.78 +0.1 407,928 - DVU0358 0.79 -0.4 407,933 - DVU0358 0.79 -0.3 407,985 + DVU0358 0.86 -0.7 407,998 - DVU0358 0.88 +0.7 408,109 + -0.4 408,116 + -0.8 408,124 - -0.2 408,142 - +2.2 408,165 - +0.8 408,313 + +0.7 408,379 - +0.6 408,482 + DVU0359 0.35 +1.5 408,514 + DVU0359 0.45 -0.1 408,551 + DVU0359 0.56 +0.4 408,628 - DVU0359 0.80 +0.2 408,846 - +0.9 408,934 + +1.2 408,942 - +0.1 408,975 + -0.3 409,019 - -1.0 409,021 + -1.2 409,022 + -0.2 409,071 + +0.3 409,116 - -0.4 409,128 - +0.1 409,148 + +0.2 409,338 + -0.4 409,345 - -0.7 409,345 - +0.5 409,346 - +0.1 409,436 + -0.5 409,486 + -0.2 409,526 + +0.2 409,534 - -0.2 409,536 + +0.1 409,536 + +0.0 409,544 - -0.1 409,544 - +0.1 409,557 + +1.9 409,576 - ilvB-1 DVU0360 0.11 -0.6 409,620 - ilvB-1 DVU0360 0.14 +0.0
Or see this region's nucleotide sequence