Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliI and DVU0311 overlap by 4 nucleotides DVU0311 and fliG overlap by 14 nucleotides fliG and fliF overlap by 32 nucleotides
DVU0310: fliI - flagellum-specific ATP synthase FliI (TIGR), at 354,912 to 356,225
fliI
DVU0311: DVU0311 - flagellar assembly protein FliH, putative (TIGR), at 356,222 to 356,974
DVU0311
DVU0312: fliG - flagellar motor switch protein FliG (TIGR), at 356,961 to 357,977
fliG
DVU0313: fliF - flagellar M-ring protein FliF (TIGR), at 357,946 to 359,562
fliF
Position (kb) 356
357
358 Strain fitness (log2 ratio) -2
-1
0
1
2
3 at 356.000 kb on + strand, within fliI at 356.008 kb on - strand, within fliI at 356.008 kb on - strand, within fliI at 356.008 kb on - strand, within fliI at 356.012 kb on - strand, within fliI at 356.013 kb on - strand, within fliI at 356.084 kb on + strand, within fliI at 356.085 kb on + strand, within fliI at 356.145 kb on - strand at 356.147 kb on - strand at 356.154 kb on - strand at 356.204 kb on - strand at 356.249 kb on - strand at 356.263 kb on - strand at 356.277 kb on - strand at 356.278 kb on - strand at 356.426 kb on + strand, within DVU0311 at 356.495 kb on + strand, within DVU0311 at 356.516 kb on + strand, within DVU0311 at 356.646 kb on + strand, within DVU0311 at 356.741 kb on - strand, within DVU0311 at 356.797 kb on + strand, within DVU0311 at 356.883 kb on + strand, within DVU0311 at 356.898 kb on - strand, within DVU0311 at 356.926 kb on - strand at 356.927 kb on + strand at 356.936 kb on - strand at 357.003 kb on + strand at 357.003 kb on + strand at 357.045 kb on - strand at 357.047 kb on - strand at 357.060 kb on - strand at 357.069 kb on - strand, within fliG at 357.181 kb on - strand, within fliG at 357.202 kb on + strand, within fliG at 357.202 kb on + strand, within fliG at 357.210 kb on - strand, within fliG at 357.366 kb on + strand, within fliG at 357.417 kb on - strand, within fliG at 357.466 kb on + strand, within fliG at 357.466 kb on + strand, within fliG at 357.601 kb on + strand, within fliG at 357.652 kb on + strand, within fliG at 357.664 kb on + strand, within fliG at 357.682 kb on - strand, within fliG at 357.710 kb on - strand, within fliG at 357.763 kb on - strand, within fliG at 357.799 kb on + strand, within fliG at 357.802 kb on - strand, within fliG at 357.802 kb on - strand, within fliG at 357.802 kb on - strand, within fliG at 357.864 kb on - strand, within fliG at 357.885 kb on + strand at 357.943 kb on + strand at 357.943 kb on + strand at 357.943 kb on - strand at 357.951 kb on - strand at 358.015 kb on - strand at 358.033 kb on + strand at 358.046 kb on + strand at 358.054 kb on - strand at 358.090 kb on - strand at 358.148 kb on - strand, within fliF at 358.188 kb on - strand, within fliF at 358.202 kb on - strand, within fliF at 358.238 kb on + strand, within fliF at 358.238 kb on + strand, within fliF at 358.274 kb on - strand, within fliF at 358.334 kb on + strand, within fliF at 358.341 kb on + strand, within fliF at 358.374 kb on - strand, within fliF at 358.435 kb on + strand, within fliF at 358.443 kb on - strand, within fliF at 358.443 kb on - strand, within fliF at 358.458 kb on + strand, within fliF at 358.504 kb on - strand, within fliF at 358.533 kb on - strand, within fliF at 358.621 kb on - strand, within fliF at 358.640 kb on + strand, within fliF at 358.683 kb on + strand, within fliF at 358.691 kb on - strand, within fliF at 358.765 kb on - strand, within fliF at 358.772 kb on - strand, within fliF at 358.796 kb on + strand, within fliF at 358.796 kb on + strand, within fliF at 358.804 kb on - strand, within fliF at 358.804 kb on - strand, within fliF at 358.967 kb on - strand, within fliF
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 356,000 + fliI DVU0310 0.83 +0.2 356,008 - fliI DVU0310 0.83 +0.6 356,008 - fliI DVU0310 0.83 +0.3 356,008 - fliI DVU0310 0.83 +1.2 356,012 - fliI DVU0310 0.84 +0.3 356,013 - fliI DVU0310 0.84 -1.0 356,084 + fliI DVU0310 0.89 +0.4 356,085 + fliI DVU0310 0.89 +0.9 356,145 - -0.2 356,147 - -0.7 356,154 - -0.2 356,204 - +0.2 356,249 - -2.2 356,263 - +1.5 356,277 - -0.4 356,278 - -0.2 356,426 + DVU0311 0.27 +0.1 356,495 + DVU0311 0.36 +0.3 356,516 + DVU0311 0.39 +0.6 356,646 + DVU0311 0.56 +2.5 356,741 - DVU0311 0.69 +0.7 356,797 + DVU0311 0.76 +0.2 356,883 + DVU0311 0.88 +1.0 356,898 - DVU0311 0.90 +0.3 356,926 - +0.3 356,927 + +1.2 356,936 - +0.2 357,003 + +0.4 357,003 + +0.3 357,045 - +0.1 357,047 - +0.2 357,060 - +0.5 357,069 - fliG DVU0312 0.11 +1.1 357,181 - fliG DVU0312 0.22 +0.5 357,202 + fliG DVU0312 0.24 +0.2 357,202 + fliG DVU0312 0.24 +0.1 357,210 - fliG DVU0312 0.24 -0.1 357,366 + fliG DVU0312 0.40 +1.0 357,417 - fliG DVU0312 0.45 +0.4 357,466 + fliG DVU0312 0.50 -1.3 357,466 + fliG DVU0312 0.50 +0.7 357,601 + fliG DVU0312 0.63 +0.4 357,652 + fliG DVU0312 0.68 +1.5 357,664 + fliG DVU0312 0.69 +0.2 357,682 - fliG DVU0312 0.71 +0.9 357,710 - fliG DVU0312 0.74 +0.8 357,763 - fliG DVU0312 0.79 +0.5 357,799 + fliG DVU0312 0.82 -0.3 357,802 - fliG DVU0312 0.83 +0.5 357,802 - fliG DVU0312 0.83 +0.5 357,802 - fliG DVU0312 0.83 +3.5 357,864 - fliG DVU0312 0.89 +0.4 357,885 + -0.7 357,943 + -0.7 357,943 + +0.4 357,943 - -0.7 357,951 - -0.4 358,015 - +0.5 358,033 + +0.3 358,046 + -0.1 358,054 - +0.3 358,090 - -0.8 358,148 - fliF DVU0313 0.12 +0.5 358,188 - fliF DVU0313 0.15 +0.5 358,202 - fliF DVU0313 0.16 -0.0 358,238 + fliF DVU0313 0.18 +0.0 358,238 + fliF DVU0313 0.18 +0.0 358,274 - fliF DVU0313 0.20 -1.8 358,334 + fliF DVU0313 0.24 -0.1 358,341 + fliF DVU0313 0.24 -0.1 358,374 - fliF DVU0313 0.26 -0.7 358,435 + fliF DVU0313 0.30 +0.1 358,443 - fliF DVU0313 0.31 +0.8 358,443 - fliF DVU0313 0.31 -0.3 358,458 + fliF DVU0313 0.32 +0.1 358,504 - fliF DVU0313 0.35 +0.3 358,533 - fliF DVU0313 0.36 +0.4 358,621 - fliF DVU0313 0.42 +2.9 358,640 + fliF DVU0313 0.43 +0.3 358,683 + fliF DVU0313 0.46 +0.5 358,691 - fliF DVU0313 0.46 -0.0 358,765 - fliF DVU0313 0.51 +0.6 358,772 - fliF DVU0313 0.51 +1.8 358,796 + fliF DVU0313 0.53 +0.3 358,796 + fliF DVU0313 0.53 -1.0 358,804 - fliF DVU0313 0.53 -0.1 358,804 - fliF DVU0313 0.53 +0.8 358,967 - fliF DVU0313 0.63 -1.1
Or see this region's nucleotide sequence