Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt livF and livG overlap by 1 nucleotides livG and livM are separated by 9 nucleotides livM and livH are separated by 22 nucleotides
DVU2740: livF - high-affinity branched-chain amino acid ABC transporter, ATP-binding protein (TIGR), at 2,845,923 to 2,846,648
livF
DVU2741: livG - high-affinity branched chain amino acid ABC transporter, ATP-binding protein (TIGR), at 2,846,648 to 2,847,415
livG
DVU2742: livM - high-affinity branched chain amino acid ABC transporter, permease protein (TIGR), at 2,847,425 to 2,848,501
livM
DVU2743: livH - high-affinity branched-chain amino acid ABC ransporter, permease protein (TIGR), at 2,848,524 to 2,849,444
livH
Position (kb)
2847
2848
2849 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2846.673 kb on + strand at 2846.727 kb on + strand, within livG at 2846.758 kb on + strand, within livG at 2846.776 kb on + strand, within livG at 2846.930 kb on + strand, within livG at 2847.021 kb on - strand, within livG at 2847.083 kb on - strand, within livG at 2847.085 kb on + strand, within livG at 2847.085 kb on + strand, within livG at 2847.087 kb on + strand, within livG at 2847.087 kb on + strand, within livG at 2847.087 kb on + strand, within livG at 2847.094 kb on - strand, within livG at 2847.095 kb on - strand, within livG at 2847.107 kb on - strand, within livG at 2847.139 kb on + strand, within livG at 2847.175 kb on - strand, within livG at 2847.179 kb on + strand, within livG at 2847.371 kb on - strand at 2847.498 kb on + strand at 2847.501 kb on - strand at 2847.506 kb on - strand at 2847.556 kb on + strand, within livM at 2847.583 kb on + strand, within livM at 2847.583 kb on + strand, within livM at 2847.583 kb on + strand, within livM at 2847.583 kb on + strand, within livM at 2847.591 kb on - strand, within livM at 2847.591 kb on - strand, within livM at 2847.591 kb on - strand, within livM at 2847.762 kb on + strand, within livM at 2847.939 kb on + strand, within livM at 2847.961 kb on + strand, within livM at 2847.995 kb on + strand, within livM at 2848.062 kb on - strand, within livM at 2848.196 kb on - strand, within livM at 2848.210 kb on - strand, within livM at 2848.219 kb on + strand, within livM at 2848.224 kb on + strand, within livM at 2848.232 kb on - strand, within livM at 2848.247 kb on - strand, within livM at 2848.363 kb on + strand, within livM at 2848.460 kb on + strand at 2848.488 kb on + strand at 2848.488 kb on + strand at 2848.494 kb on - strand at 2848.502 kb on + strand at 2848.522 kb on + strand at 2848.556 kb on + strand at 2848.564 kb on - strand at 2848.587 kb on - strand at 2848.587 kb on - strand at 2848.623 kb on + strand, within livH at 2848.768 kb on + strand, within livH at 2848.768 kb on + strand, within livH at 2848.768 kb on + strand, within livH at 2848.776 kb on - strand, within livH at 2848.776 kb on - strand, within livH at 2848.780 kb on + strand, within livH at 2848.788 kb on - strand, within livH at 2848.835 kb on - strand, within livH at 2848.848 kb on - strand, within livH at 2848.932 kb on + strand, within livH at 2849.002 kb on + strand, within livH at 2849.019 kb on - strand, within livH at 2849.145 kb on + strand, within livH at 2849.223 kb on + strand, within livH at 2849.230 kb on - strand, within livH at 2849.284 kb on - strand, within livH at 2849.322 kb on - strand, within livH at 2849.355 kb on + strand at 2849.356 kb on + strand at 2849.356 kb on + strand at 2849.356 kb on - strand at 2849.368 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,846,673 + -1.3 2,846,727 + livG DVU2741 0.10 -0.2 2,846,758 + livG DVU2741 0.14 -0.2 2,846,776 + livG DVU2741 0.17 +1.0 2,846,930 + livG DVU2741 0.37 -0.9 2,847,021 - livG DVU2741 0.49 +2.1 2,847,083 - livG DVU2741 0.57 -0.4 2,847,085 + livG DVU2741 0.57 -0.9 2,847,085 + livG DVU2741 0.57 +2.6 2,847,087 + livG DVU2741 0.57 -0.4 2,847,087 + livG DVU2741 0.57 -0.3 2,847,087 + livG DVU2741 0.57 -0.2 2,847,094 - livG DVU2741 0.58 -0.1 2,847,095 - livG DVU2741 0.58 +0.2 2,847,107 - livG DVU2741 0.60 -0.4 2,847,139 + livG DVU2741 0.64 -0.5 2,847,175 - livG DVU2741 0.69 -0.1 2,847,179 + livG DVU2741 0.69 -0.7 2,847,371 - -0.9 2,847,498 + +0.6 2,847,501 - +1.6 2,847,506 - -1.4 2,847,556 + livM DVU2742 0.12 +0.9 2,847,583 + livM DVU2742 0.15 +1.6 2,847,583 + livM DVU2742 0.15 -0.2 2,847,583 + livM DVU2742 0.15 +1.3 2,847,583 + livM DVU2742 0.15 -0.4 2,847,591 - livM DVU2742 0.15 +1.2 2,847,591 - livM DVU2742 0.15 +1.2 2,847,591 - livM DVU2742 0.15 -0.4 2,847,762 + livM DVU2742 0.31 +3.6 2,847,939 + livM DVU2742 0.48 +0.0 2,847,961 + livM DVU2742 0.50 +0.7 2,847,995 + livM DVU2742 0.53 -0.1 2,848,062 - livM DVU2742 0.59 -0.7 2,848,196 - livM DVU2742 0.72 +1.0 2,848,210 - livM DVU2742 0.73 -0.7 2,848,219 + livM DVU2742 0.74 -0.0 2,848,224 + livM DVU2742 0.74 +0.1 2,848,232 - livM DVU2742 0.75 +0.5 2,848,247 - livM DVU2742 0.76 +1.0 2,848,363 + livM DVU2742 0.87 -0.1 2,848,460 + -0.1 2,848,488 + -0.4 2,848,488 + -0.6 2,848,494 - -0.4 2,848,502 + -0.6 2,848,522 + +0.7 2,848,556 + -0.4 2,848,564 - +0.6 2,848,587 - +0.5 2,848,587 - -0.5 2,848,623 + livH DVU2743 0.11 +0.0 2,848,768 + livH DVU2743 0.26 -1.1 2,848,768 + livH DVU2743 0.26 +0.3 2,848,768 + livH DVU2743 0.26 +0.3 2,848,776 - livH DVU2743 0.27 -0.4 2,848,776 - livH DVU2743 0.27 -0.4 2,848,780 + livH DVU2743 0.28 -0.8 2,848,788 - livH DVU2743 0.29 -0.9 2,848,835 - livH DVU2743 0.34 -0.4 2,848,848 - livH DVU2743 0.35 -0.3 2,848,932 + livH DVU2743 0.44 -0.7 2,849,002 + livH DVU2743 0.52 -0.1 2,849,019 - livH DVU2743 0.54 +1.0 2,849,145 + livH DVU2743 0.67 -0.1 2,849,223 + livH DVU2743 0.76 +0.4 2,849,230 - livH DVU2743 0.77 +0.8 2,849,284 - livH DVU2743 0.83 -0.8 2,849,322 - livH DVU2743 0.87 -2.4 2,849,355 + -0.0 2,849,356 + +0.0 2,849,356 + +0.2 2,849,356 - -0.4 2,849,368 + -0.3
Or see this region's nucleotide sequence