Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2360 and thiE-2 are separated by 223 nucleotides thiE-2 and thiM overlap by 4 nucleotides
DVU2360: DVU2360 - oxidoreductase, FAD/NAD-binding family (TIGR), at 2,458,415 to 2,459,221
DVU2360
DVU2362: thiE-2 - thiamine-phosphate pyrophosphorylase (TIGR), at 2,459,445 to 2,460,104
thiE-2
DVU2363: thiM - hydroxyethylthiazole kinase (TIGR), at 2,460,101 to 2,460,904
thiM
Position (kb)
2459
2460
2461 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2458.528 kb on + strand, within DVU2360 at 2458.558 kb on + strand, within DVU2360 at 2458.627 kb on + strand, within DVU2360 at 2458.634 kb on + strand, within DVU2360 at 2458.637 kb on - strand, within DVU2360 at 2458.677 kb on - strand, within DVU2360 at 2458.711 kb on - strand, within DVU2360 at 2458.801 kb on + strand, within DVU2360 at 2458.839 kb on - strand, within DVU2360 at 2458.895 kb on + strand, within DVU2360 at 2458.908 kb on - strand, within DVU2360 at 2458.930 kb on + strand, within DVU2360 at 2458.965 kb on + strand, within DVU2360 at 2458.973 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.022 kb on - strand, within DVU2360 at 2459.086 kb on - strand, within DVU2360 at 2459.121 kb on - strand, within DVU2360 at 2459.180 kb on + strand at 2459.188 kb on - strand at 2459.204 kb on + strand at 2459.229 kb on + strand at 2459.237 kb on - strand at 2459.237 kb on - strand at 2459.329 kb on + strand at 2459.369 kb on + strand at 2459.370 kb on + strand at 2459.403 kb on - strand at 2459.440 kb on - strand at 2459.485 kb on - strand at 2459.578 kb on + strand, within thiE-2 at 2459.578 kb on + strand, within thiE-2 at 2459.727 kb on + strand, within thiE-2 at 2459.748 kb on - strand, within thiE-2 at 2459.978 kb on + strand, within thiE-2 at 2460.052 kb on - strand at 2460.061 kb on + strand at 2460.103 kb on - strand at 2460.122 kb on - strand at 2460.314 kb on + strand, within thiM at 2460.322 kb on - strand, within thiM at 2460.322 kb on - strand, within thiM at 2460.412 kb on - strand, within thiM at 2460.468 kb on + strand, within thiM at 2460.477 kb on - strand, within thiM at 2460.487 kb on - strand, within thiM at 2460.494 kb on - strand, within thiM at 2460.494 kb on - strand, within thiM at 2460.504 kb on + strand, within thiM at 2460.571 kb on - strand, within thiM at 2460.597 kb on + strand, within thiM at 2460.597 kb on + strand, within thiM at 2460.605 kb on - strand, within thiM at 2460.637 kb on + strand, within thiM at 2460.675 kb on - strand, within thiM at 2460.791 kb on - strand, within thiM at 2460.827 kb on - strand at 2460.892 kb on - strand at 2460.902 kb on + strand at 2460.902 kb on - strand at 2460.914 kb on + strand at 2460.947 kb on - strand at 2460.947 kb on - strand at 2461.065 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,458,528 + DVU2360 0.14 +0.3 2,458,558 + DVU2360 0.18 -1.1 2,458,627 + DVU2360 0.26 -0.2 2,458,634 + DVU2360 0.27 +0.8 2,458,637 - DVU2360 0.28 -0.6 2,458,677 - DVU2360 0.32 -0.3 2,458,711 - DVU2360 0.37 +0.0 2,458,801 + DVU2360 0.48 +0.1 2,458,839 - DVU2360 0.53 -0.1 2,458,895 + DVU2360 0.59 -0.4 2,458,908 - DVU2360 0.61 -1.2 2,458,930 + DVU2360 0.64 +0.1 2,458,965 + DVU2360 0.68 -0.3 2,458,973 - DVU2360 0.69 +1.1 2,459,022 - DVU2360 0.75 -0.2 2,459,022 - DVU2360 0.75 -0.3 2,459,022 - DVU2360 0.75 +0.7 2,459,086 - DVU2360 0.83 +0.2 2,459,121 - DVU2360 0.87 -0.4 2,459,180 + +1.6 2,459,188 - +2.2 2,459,204 + -0.6 2,459,229 + -1.3 2,459,237 - +1.1 2,459,237 - -1.0 2,459,329 + -0.0 2,459,369 + +0.6 2,459,370 + +0.6 2,459,403 - +2.6 2,459,440 - -0.1 2,459,485 - -0.4 2,459,578 + thiE-2 DVU2362 0.20 +1.8 2,459,578 + thiE-2 DVU2362 0.20 +3.1 2,459,727 + thiE-2 DVU2362 0.43 -0.2 2,459,748 - thiE-2 DVU2362 0.46 +0.3 2,459,978 + thiE-2 DVU2362 0.81 +1.0 2,460,052 - -0.8 2,460,061 + +0.4 2,460,103 - +0.3 2,460,122 - +0.1 2,460,314 + thiM DVU2363 0.26 -0.3 2,460,322 - thiM DVU2363 0.27 +0.6 2,460,322 - thiM DVU2363 0.27 -0.3 2,460,412 - thiM DVU2363 0.39 -0.2 2,460,468 + thiM DVU2363 0.46 -0.4 2,460,477 - thiM DVU2363 0.47 -0.2 2,460,487 - thiM DVU2363 0.48 +0.8 2,460,494 - thiM DVU2363 0.49 -0.6 2,460,494 - thiM DVU2363 0.49 -0.2 2,460,504 + thiM DVU2363 0.50 +0.1 2,460,571 - thiM DVU2363 0.58 +2.1 2,460,597 + thiM DVU2363 0.62 -2.2 2,460,597 + thiM DVU2363 0.62 -0.6 2,460,605 - thiM DVU2363 0.63 +0.5 2,460,637 + thiM DVU2363 0.67 +0.0 2,460,675 - thiM DVU2363 0.71 -0.0 2,460,791 - thiM DVU2363 0.86 +0.3 2,460,827 - +0.1 2,460,892 - -0.1 2,460,902 + +0.0 2,460,902 - -2.0 2,460,914 + -0.4 2,460,947 - -0.1 2,460,947 - -0.7 2,461,065 + +0.1
Or see this region's nucleotide sequence