Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0449 and tRNA-Leu are separated by 279 nucleotides tRNA-Leu and ribF are separated by 482 nucleotides
DVU0449: DVU0449 - sensor/response regulator (TIGR), at 509,426 to 513,967
DVU0449
DVU_tRNA-Leu-2: tRNA-Leu - tRNA with anticodon CAG for Leu (VIMSS-AUTO), at 514,247 to 514,333
tRNA-Leu
DVU0450: ribF - riboflavin biosynthesis protein RibF (TIGR), at 514,816 to 515,760
ribF
Position (kb)
514
515 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 513.249 kb on - strand, within DVU0449 at 513.314 kb on + strand, within DVU0449 at 513.319 kb on + strand, within DVU0449 at 513.319 kb on + strand, within DVU0449 at 513.422 kb on + strand, within DVU0449 at 513.468 kb on - strand, within DVU0449 at 513.526 kb on + strand at 513.549 kb on - strand at 513.589 kb on - strand at 513.622 kb on + strand at 513.622 kb on + strand at 513.630 kb on - strand at 513.650 kb on - strand at 513.696 kb on + strand at 513.719 kb on + strand at 513.719 kb on + strand at 513.719 kb on + strand at 513.801 kb on + strand at 513.825 kb on - strand at 514.002 kb on + strand at 514.068 kb on + strand at 514.068 kb on + strand at 514.068 kb on + strand at 514.076 kb on - strand at 514.089 kb on + strand at 514.089 kb on + strand at 514.091 kb on - strand at 514.141 kb on - strand at 514.170 kb on + strand at 514.420 kb on - strand at 514.431 kb on - strand at 514.556 kb on - strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.569 kb on - strand at 514.569 kb on - strand at 514.569 kb on - strand at 514.576 kb on + strand at 514.584 kb on - strand at 514.585 kb on + strand at 514.592 kb on - strand at 514.631 kb on + strand at 514.653 kb on + strand at 514.694 kb on - strand at 514.710 kb on + strand at 514.739 kb on + strand at 514.749 kb on + strand at 514.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 513,249 - DVU0449 0.84 -0.1 513,314 + DVU0449 0.86 +1.0 513,319 + DVU0449 0.86 +4.7 513,319 + DVU0449 0.86 -0.3 513,422 + DVU0449 0.88 -1.0 513,468 - DVU0449 0.89 -0.3 513,526 + +3.1 513,549 - +0.8 513,589 - -1.0 513,622 + +2.7 513,622 + -0.5 513,630 - -2.5 513,650 - -0.1 513,696 + -1.2 513,719 + +0.3 513,719 + +1.4 513,719 + -1.0 513,801 + -0.8 513,825 - -2.2 514,002 + +1.2 514,068 + -0.4 514,068 + -1.0 514,068 + -1.1 514,076 - -0.9 514,089 + -0.9 514,089 + +0.8 514,091 - -0.5 514,141 - -1.4 514,170 + -1.5 514,420 - +3.7 514,431 - -0.3 514,556 - -1.8 514,561 + -0.6 514,561 + -1.9 514,561 + -2.2 514,561 + -0.9 514,569 - -1.4 514,569 - +0.8 514,569 - +3.7 514,576 + -0.9 514,584 - -1.8 514,585 + -1.8 514,592 - -0.6 514,631 + -0.3 514,653 + -0.7 514,694 - -1.5 514,710 + -1.2 514,739 + -0.3 514,749 + -1.4 514,802 - -1.6
Or see this region's nucleotide sequence