Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0052 and DVUA0053 are separated by 250 nucleotides DVUA0053 and DVUA0054 overlap by 4 nucleotides
DVUA0052: DVUA0052 - conserved hypothetical protein (TIGR), at 64,069 to 64,737
DVUA0052
DVUA0053: DVUA0053 - conserved hypothetical protein (TIGR), at 64,988 to 65,575
DVUA0053
DVUA0054: DVUA0054 - glycosyl transferase, group 1 family protein (TIGR), at 65,572 to 66,972
DVUA0054
Position (kb)
64
65
66 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 64.031 kb on - strand at 64.208 kb on + strand, within DVUA0052 at 64.208 kb on + strand, within DVUA0052 at 64.208 kb on + strand, within DVUA0052 at 64.208 kb on + strand, within DVUA0052 at 64.216 kb on - strand, within DVUA0052 at 64.216 kb on - strand, within DVUA0052 at 64.236 kb on + strand, within DVUA0052 at 64.236 kb on + strand, within DVUA0052 at 64.282 kb on + strand, within DVUA0052 at 64.333 kb on + strand, within DVUA0052 at 64.361 kb on - strand, within DVUA0052 at 64.364 kb on - strand, within DVUA0052 at 64.413 kb on + strand, within DVUA0052 at 64.445 kb on + strand, within DVUA0052 at 64.445 kb on + strand, within DVUA0052 at 64.458 kb on - strand, within DVUA0052 at 64.518 kb on + strand, within DVUA0052 at 64.650 kb on + strand, within DVUA0052 at 64.701 kb on + strand at 64.701 kb on + strand at 64.709 kb on - strand at 64.714 kb on + strand at 64.717 kb on - strand at 64.724 kb on + strand at 64.732 kb on + strand at 64.732 kb on + strand at 64.740 kb on - strand at 64.768 kb on - strand at 64.779 kb on - strand at 64.779 kb on - strand at 64.781 kb on + strand at 64.835 kb on + strand at 65.030 kb on + strand at 65.035 kb on + strand at 65.038 kb on - strand at 65.043 kb on - strand at 65.121 kb on + strand, within DVUA0053 at 65.138 kb on + strand, within DVUA0053 at 65.153 kb on + strand, within DVUA0053 at 65.161 kb on - strand, within DVUA0053 at 65.259 kb on - strand, within DVUA0053 at 65.264 kb on - strand, within DVUA0053 at 65.327 kb on + strand, within DVUA0053 at 65.444 kb on + strand, within DVUA0053 at 65.452 kb on - strand, within DVUA0053 at 65.516 kb on + strand, within DVUA0053 at 65.597 kb on + strand at 65.605 kb on - strand at 65.611 kb on - strand at 65.637 kb on + strand at 65.649 kb on - strand at 65.895 kb on + strand, within DVUA0054 at 65.903 kb on - strand, within DVUA0054 at 65.903 kb on - strand, within DVUA0054 at 65.903 kb on - strand, within DVUA0054 at 65.921 kb on + strand, within DVUA0054 at 65.929 kb on - strand, within DVUA0054 at 65.980 kb on + strand, within DVUA0054 at 66.012 kb on + strand, within DVUA0054 at 66.045 kb on - strand, within DVUA0054 at 66.055 kb on - strand, within DVUA0054 at 66.147 kb on - strand, within DVUA0054 at 66.149 kb on + strand, within DVUA0054 at 66.221 kb on + strand, within DVUA0054 at 66.292 kb on + strand, within DVUA0054 at 66.344 kb on - strand, within DVUA0054 at 66.401 kb on - strand, within DVUA0054 at 66.413 kb on + strand, within DVUA0054 at 66.464 kb on - strand, within DVUA0054 at 66.479 kb on - strand, within DVUA0054 at 66.487 kb on - strand, within DVUA0054 at 66.507 kb on + strand, within DVUA0054 at 66.521 kb on + strand, within DVUA0054 at 66.543 kb on + strand, within DVUA0054 at 66.574 kb on - strand, within DVUA0054
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 64,031 - +1.9 64,208 + DVUA0052 0.21 -2.4 64,208 + DVUA0052 0.21 -2.6 64,208 + DVUA0052 0.21 -0.9 64,208 + DVUA0052 0.21 -1.6 64,216 - DVUA0052 0.22 -2.6 64,216 - DVUA0052 0.22 -1.9 64,236 + DVUA0052 0.25 -1.7 64,236 + DVUA0052 0.25 -1.1 64,282 + DVUA0052 0.32 +1.7 64,333 + DVUA0052 0.39 +0.5 64,361 - DVUA0052 0.44 -1.1 64,364 - DVUA0052 0.44 -0.2 64,413 + DVUA0052 0.51 +0.8 64,445 + DVUA0052 0.56 -0.6 64,445 + DVUA0052 0.56 -0.1 64,458 - DVUA0052 0.58 -0.4 64,518 + DVUA0052 0.67 +0.9 64,650 + DVUA0052 0.87 -0.4 64,701 + -1.4 64,701 + -2.2 64,709 - -1.9 64,714 + +1.2 64,717 - -1.3 64,724 + +2.7 64,732 + -1.2 64,732 + -1.9 64,740 - -1.1 64,768 - +2.1 64,779 - -0.3 64,779 - -1.2 64,781 + +2.3 64,835 + +0.3 65,030 + -1.3 65,035 + -1.7 65,038 - -1.7 65,043 - +2.1 65,121 + DVUA0053 0.23 -2.3 65,138 + DVUA0053 0.26 -0.1 65,153 + DVUA0053 0.28 +1.4 65,161 - DVUA0053 0.29 -2.2 65,259 - DVUA0053 0.46 +0.9 65,264 - DVUA0053 0.47 -1.5 65,327 + DVUA0053 0.58 +1.7 65,444 + DVUA0053 0.78 -0.4 65,452 - DVUA0053 0.79 -1.1 65,516 + DVUA0053 0.90 -1.2 65,597 + +0.8 65,605 - -1.1 65,611 - -0.4 65,637 + -1.5 65,649 - +0.6 65,895 + DVUA0054 0.23 -1.5 65,903 - DVUA0054 0.24 +0.6 65,903 - DVUA0054 0.24 -0.2 65,903 - DVUA0054 0.24 +3.7 65,921 + DVUA0054 0.25 -2.0 65,929 - DVUA0054 0.25 +0.7 65,980 + DVUA0054 0.29 +0.1 66,012 + DVUA0054 0.31 -1.7 66,045 - DVUA0054 0.34 +1.7 66,055 - DVUA0054 0.34 -0.1 66,147 - DVUA0054 0.41 -1.3 66,149 + DVUA0054 0.41 +0.7 66,221 + DVUA0054 0.46 -1.6 66,292 + DVUA0054 0.51 -2.0 66,344 - DVUA0054 0.55 -1.2 66,401 - DVUA0054 0.59 -0.5 66,413 + DVUA0054 0.60 +0.1 66,464 - DVUA0054 0.64 -1.2 66,479 - DVUA0054 0.65 +1.0 66,487 - DVUA0054 0.65 -0.5 66,507 + DVUA0054 0.67 -1.8 66,521 + DVUA0054 0.68 +2.1 66,543 + DVUA0054 0.69 -0.6 66,574 - DVUA0054 0.72 +1.9
Or see this region's nucleotide sequence