Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysW and cysA overlap by 4 nucleotides cysA and acs are separated by 85 nucleotides acs and DVU0749 are separated by 49 nucleotides
DVU0746: cysW - ABC transporter, permease protein (TIGR), at 835,639 to 836,385
cysW
DVU0747: cysA - ABC transporter, ATP-binding protein (TIGR), at 836,382 to 837,020
cysA
DVU0748: acs - acetyl-CoA synthetase (TIGR), at 837,106 to 839,052
acs
DVU0749: DVU0749 - DNA-binding response regulator (TIGR), at 839,102 to 840,031
DVU0749
Position (kb) 837
838
839
840 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3 at 836.143 kb on + strand, within cysW at 836.372 kb on + strand at 836.709 kb on + strand, within cysA at 836.751 kb on - strand, within cysA at 836.838 kb on + strand, within cysA at 836.843 kb on + strand, within cysA at 836.871 kb on + strand, within cysA at 836.953 kb on + strand, within cysA at 837.048 kb on - strand at 837.118 kb on - strand at 837.136 kb on - strand at 837.145 kb on + strand at 837.228 kb on - strand at 837.268 kb on - strand at 837.276 kb on + strand at 837.287 kb on + strand at 837.430 kb on - strand, within acs at 837.787 kb on + strand, within acs at 837.810 kb on + strand, within acs at 837.818 kb on - strand, within acs at 837.826 kb on + strand, within acs at 837.905 kb on + strand, within acs at 837.905 kb on + strand, within acs at 837.907 kb on + strand, within acs at 837.960 kb on + strand, within acs at 837.961 kb on - strand, within acs at 838.066 kb on + strand, within acs at 838.086 kb on - strand, within acs at 838.101 kb on + strand, within acs at 838.109 kb on - strand, within acs at 838.162 kb on + strand, within acs at 838.202 kb on + strand, within acs at 838.210 kb on - strand, within acs at 838.370 kb on + strand, within acs at 838.430 kb on + strand, within acs at 838.485 kb on + strand, within acs at 838.485 kb on + strand, within acs at 838.547 kb on + strand, within acs at 838.593 kb on - strand, within acs at 838.618 kb on + strand, within acs at 838.714 kb on + strand, within acs at 838.807 kb on - strand, within acs at 838.930 kb on - strand at 838.953 kb on + strand at 838.984 kb on + strand at 839.163 kb on - strand at 839.163 kb on - strand at 839.396 kb on - strand, within DVU0749 at 839.465 kb on - strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.469 kb on + strand, within DVU0749 at 839.487 kb on - strand, within DVU0749 at 839.487 kb on - strand, within DVU0749 at 839.521 kb on + strand, within DVU0749 at 839.543 kb on + strand, within DVU0749 at 839.543 kb on + strand, within DVU0749 at 839.680 kb on + strand, within DVU0749 at 839.681 kb on + strand, within DVU0749 at 839.861 kb on - strand, within DVU0749 at 839.885 kb on + strand, within DVU0749 at 839.885 kb on + strand, within DVU0749 at 839.905 kb on - strand, within DVU0749 at 839.912 kb on - strand, within DVU0749 at 839.932 kb on + strand, within DVU0749 at 839.989 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 836,143 + cysW DVU0746 0.67 +0.2 836,372 + -0.3 836,709 + cysA DVU0747 0.51 -1.1 836,751 - cysA DVU0747 0.58 +1.9 836,838 + cysA DVU0747 0.71 +0.4 836,843 + cysA DVU0747 0.72 -1.1 836,871 + cysA DVU0747 0.77 +0.2 836,953 + cysA DVU0747 0.89 -0.9 837,048 - +1.2 837,118 - +0.4 837,136 - +1.4 837,145 + +0.1 837,228 - -1.4 837,268 - +1.6 837,276 + -0.4 837,287 + +2.7 837,430 - acs DVU0748 0.17 -1.4 837,787 + acs DVU0748 0.35 +0.8 837,810 + acs DVU0748 0.36 -1.5 837,818 - acs DVU0748 0.37 +2.7 837,826 + acs DVU0748 0.37 +2.2 837,905 + acs DVU0748 0.41 +3.7 837,905 + acs DVU0748 0.41 -1.6 837,907 + acs DVU0748 0.41 +0.1 837,960 + acs DVU0748 0.44 -0.5 837,961 - acs DVU0748 0.44 -1.3 838,066 + acs DVU0748 0.49 -1.4 838,086 - acs DVU0748 0.50 +0.9 838,101 + acs DVU0748 0.51 -1.0 838,109 - acs DVU0748 0.52 -3.0 838,162 + acs DVU0748 0.54 -0.4 838,202 + acs DVU0748 0.56 +0.9 838,210 - acs DVU0748 0.57 -0.8 838,370 + acs DVU0748 0.65 -0.2 838,430 + acs DVU0748 0.68 +2.2 838,485 + acs DVU0748 0.71 +2.2 838,485 + acs DVU0748 0.71 +0.5 838,547 + acs DVU0748 0.74 +2.4 838,593 - acs DVU0748 0.76 -1.6 838,618 + acs DVU0748 0.78 +1.2 838,714 + acs DVU0748 0.83 -0.3 838,807 - acs DVU0748 0.87 +2.2 838,930 - +1.4 838,953 + -0.3 838,984 + -1.6 839,163 - -1.0 839,163 - +0.6 839,396 - DVU0749 0.32 +1.2 839,465 - DVU0749 0.39 -0.2 839,469 + DVU0749 0.39 +2.4 839,469 + DVU0749 0.39 -1.7 839,469 + DVU0749 0.39 -0.3 839,487 - DVU0749 0.41 +0.2 839,487 - DVU0749 0.41 -1.2 839,521 + DVU0749 0.45 +0.4 839,543 + DVU0749 0.47 -1.1 839,543 + DVU0749 0.47 -0.5 839,680 + DVU0749 0.62 -1.1 839,681 + DVU0749 0.62 -0.7 839,861 - DVU0749 0.82 +3.2 839,885 + DVU0749 0.84 -0.8 839,885 + DVU0749 0.84 +1.2 839,905 - DVU0749 0.86 -1.3 839,912 - DVU0749 0.87 -0.2 839,932 + DVU0749 0.89 +0.3 839,989 + +0.7
Or see this region's nucleotide sequence