Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0021 and DVUA0022 are separated by 8 nucleotides DVUA0022 and DVUA0023 are separated by 98 nucleotides DVUA0023 and atoC are separated by 71 nucleotides
DVUA0021: DVUA0021 - conserved hypothetical protein (TIGR), at 26,632 to 27,693
DVUA0021
DVUA0022: DVUA0022 - ABC transporter, ATP-binding protein (TIGR), at 27,702 to 28,388
DVUA0022
DVUA0023: DVUA0023 - ABC transporter, permease protein, putative (TIGR), at 28,487 to 29,617
DVUA0023
DVUA0024: atoC - sigma-54 interaction domain protein (TIGR), at 29,689 to 30,828
atoC
Position (kb) 28
29
30 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 27.565 kb on + strand, within DVUA0021 at 27.700 kb on + strand at 27.703 kb on - strand at 27.756 kb on + strand at 27.938 kb on + strand, within DVUA0022 at 28.046 kb on + strand, within DVUA0022 at 28.046 kb on + strand, within DVUA0022 at 28.046 kb on + strand, within DVUA0022 at 28.046 kb on + strand, within DVUA0022 at 28.046 kb on + strand, within DVUA0022 at 28.054 kb on - strand, within DVUA0022 at 28.054 kb on - strand, within DVUA0022 at 28.054 kb on - strand, within DVUA0022 at 28.058 kb on + strand, within DVUA0022 at 28.066 kb on - strand, within DVUA0022 at 28.066 kb on - strand, within DVUA0022 at 28.066 kb on - strand, within DVUA0022 at 28.066 kb on - strand, within DVUA0022 at 28.067 kb on + strand, within DVUA0022 at 28.072 kb on + strand, within DVUA0022 at 28.080 kb on - strand, within DVUA0022 at 28.094 kb on + strand, within DVUA0022 at 28.173 kb on - strand, within DVUA0022 at 28.199 kb on + strand, within DVUA0022 at 28.259 kb on - strand, within DVUA0022 at 28.259 kb on - strand, within DVUA0022 at 28.277 kb on - strand, within DVUA0022 at 28.277 kb on - strand, within DVUA0022 at 28.322 kb on - strand at 28.364 kb on + strand at 28.372 kb on - strand at 28.416 kb on + strand at 28.492 kb on - strand at 28.495 kb on - strand at 28.502 kb on - strand at 28.520 kb on + strand at 28.576 kb on + strand at 28.675 kb on - strand, within DVUA0023 at 28.679 kb on + strand, within DVUA0023 at 28.726 kb on - strand, within DVUA0023 at 28.757 kb on - strand, within DVUA0023 at 28.830 kb on + strand, within DVUA0023 at 28.880 kb on - strand, within DVUA0023 at 29.000 kb on - strand, within DVUA0023 at 29.002 kb on + strand, within DVUA0023 at 29.136 kb on + strand, within DVUA0023 at 29.164 kb on + strand, within DVUA0023 at 29.425 kb on - strand, within DVUA0023 at 29.465 kb on + strand, within DVUA0023 at 29.465 kb on - strand, within DVUA0023 at 29.473 kb on - strand, within DVUA0023 at 29.543 kb on + strand at 29.543 kb on - strand at 29.629 kb on + strand at 29.735 kb on - strand at 29.783 kb on + strand at 29.783 kb on + strand at 29.811 kb on - strand, within atoC at 29.876 kb on + strand, within atoC at 29.876 kb on + strand, within atoC at 29.884 kb on - strand, within atoC at 29.886 kb on - strand, within atoC at 29.968 kb on + strand, within atoC at 30.080 kb on + strand, within atoC at 30.080 kb on + strand, within atoC at 30.131 kb on + strand, within atoC at 30.167 kb on - strand, within atoC at 30.172 kb on + strand, within atoC at 30.218 kb on + strand, within atoC at 30.246 kb on + strand, within atoC at 30.367 kb on - strand, within atoC at 30.441 kb on + strand, within atoC at 30.488 kb on + strand, within atoC at 30.501 kb on - strand, within atoC at 30.587 kb on + strand, within atoC
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 27,565 + DVUA0021 0.88 +2.7 27,700 + +3.1 27,703 - +2.3 27,756 + +0.3 27,938 + DVUA0022 0.34 -1.7 28,046 + DVUA0022 0.50 -2.8 28,046 + DVUA0022 0.50 -1.1 28,046 + DVUA0022 0.50 +2.3 28,046 + DVUA0022 0.50 +1.3 28,046 + DVUA0022 0.50 -1.2 28,054 - DVUA0022 0.51 -1.8 28,054 - DVUA0022 0.51 -1.4 28,054 - DVUA0022 0.51 -0.4 28,058 + DVUA0022 0.52 -2.1 28,066 - DVUA0022 0.53 -1.2 28,066 - DVUA0022 0.53 -1.5 28,066 - DVUA0022 0.53 +2.7 28,066 - DVUA0022 0.53 -0.6 28,067 + DVUA0022 0.53 -1.7 28,072 + DVUA0022 0.54 +2.3 28,080 - DVUA0022 0.55 -1.6 28,094 + DVUA0022 0.57 +1.7 28,173 - DVUA0022 0.69 -0.5 28,199 + DVUA0022 0.72 -0.5 28,259 - DVUA0022 0.81 -2.0 28,259 - DVUA0022 0.81 -1.0 28,277 - DVUA0022 0.84 +0.7 28,277 - DVUA0022 0.84 +1.5 28,322 - -0.4 28,364 + +1.0 28,372 - +1.7 28,416 + -0.9 28,492 - +4.7 28,495 - -1.8 28,502 - +0.7 28,520 + +1.1 28,576 + +1.4 28,675 - DVUA0023 0.17 +0.7 28,679 + DVUA0023 0.17 -0.7 28,726 - DVUA0023 0.21 -0.9 28,757 - DVUA0023 0.24 +0.3 28,830 + DVUA0023 0.30 +2.3 28,880 - DVUA0023 0.35 -0.3 29,000 - DVUA0023 0.45 +1.3 29,002 + DVUA0023 0.46 -0.6 29,136 + DVUA0023 0.57 -1.3 29,164 + DVUA0023 0.60 +1.6 29,425 - DVUA0023 0.83 +0.2 29,465 + DVUA0023 0.86 -1.1 29,465 - DVUA0023 0.86 -1.8 29,473 - DVUA0023 0.87 +3.1 29,543 + +1.5 29,543 - -0.5 29,629 + -2.0 29,735 - -1.7 29,783 + -1.8 29,783 + -1.3 29,811 - atoC DVUA0024 0.11 -0.0 29,876 + atoC DVUA0024 0.16 +2.1 29,876 + atoC DVUA0024 0.16 -1.3 29,884 - atoC DVUA0024 0.17 -1.7 29,886 - atoC DVUA0024 0.17 +1.3 29,968 + atoC DVUA0024 0.24 +0.8 30,080 + atoC DVUA0024 0.34 -3.0 30,080 + atoC DVUA0024 0.34 +1.5 30,131 + atoC DVUA0024 0.39 -0.6 30,167 - atoC DVUA0024 0.42 -1.6 30,172 + atoC DVUA0024 0.42 +0.8 30,218 + atoC DVUA0024 0.46 -1.8 30,246 + atoC DVUA0024 0.49 -2.0 30,367 - atoC DVUA0024 0.59 -0.9 30,441 + atoC DVUA0024 0.66 -2.1 30,488 + atoC DVUA0024 0.70 +0.3 30,501 - atoC DVUA0024 0.71 -0.4 30,587 + atoC DVUA0024 0.79 +1.2
Or see this region's nucleotide sequence