Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVUA0054 and DVUA0055 overlap by 4 nucleotides DVUA0055 and DVUA0056 overlap by 11 nucleotides DVUA0056 and atoC are separated by 154 nucleotides
DVUA0054: DVUA0054 - glycosyl transferase, group 1 family protein (TIGR), at 65,572 to 66,972
DVUA0054
DVUA0055: DVUA0055 - membrane protein, putative (TIGR), at 66,969 to 67,922
DVUA0055
DVUA0056: DVUA0056 - hypothetical protein (TIGR), at 67,912 to 68,532
DVUA0056
DVUA0057: atoC - sigma-54 dependent transcriptional regulator/response regulator (TIGR), at 68,687 to 70,117
atoC
Position (kb) 66
67
68 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 65.980 kb on + strand, within DVUA0054 at 66.012 kb on + strand, within DVUA0054 at 66.045 kb on - strand, within DVUA0054 at 66.055 kb on - strand, within DVUA0054 at 66.147 kb on - strand, within DVUA0054 at 66.149 kb on + strand, within DVUA0054 at 66.221 kb on + strand, within DVUA0054 at 66.292 kb on + strand, within DVUA0054 at 66.344 kb on - strand, within DVUA0054 at 66.401 kb on - strand, within DVUA0054 at 66.413 kb on + strand, within DVUA0054 at 66.464 kb on - strand, within DVUA0054 at 66.479 kb on - strand, within DVUA0054 at 66.487 kb on - strand, within DVUA0054 at 66.507 kb on + strand, within DVUA0054 at 66.521 kb on + strand, within DVUA0054 at 66.543 kb on + strand, within DVUA0054 at 66.574 kb on - strand, within DVUA0054 at 66.651 kb on - strand, within DVUA0054 at 66.743 kb on + strand, within DVUA0054 at 66.743 kb on + strand, within DVUA0054 at 66.751 kb on - strand, within DVUA0054 at 66.769 kb on - strand, within DVUA0054 at 66.907 kb on + strand at 66.921 kb on + strand at 67.007 kb on - strand at 67.061 kb on + strand at 67.102 kb on + strand, within DVUA0055 at 67.102 kb on + strand, within DVUA0055 at 67.105 kb on + strand, within DVUA0055 at 67.108 kb on - strand, within DVUA0055 at 67.117 kb on + strand, within DVUA0055 at 67.125 kb on - strand, within DVUA0055 at 67.137 kb on + strand, within DVUA0055 at 67.172 kb on - strand, within DVUA0055 at 67.231 kb on + strand, within DVUA0055 at 67.231 kb on + strand, within DVUA0055 at 67.239 kb on - strand, within DVUA0055 at 67.239 kb on - strand, within DVUA0055 at 67.275 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.370 kb on + strand, within DVUA0055 at 67.378 kb on - strand, within DVUA0055 at 67.378 kb on - strand, within DVUA0055 at 67.446 kb on + strand, within DVUA0055 at 67.651 kb on + strand, within DVUA0055 at 67.803 kb on + strand, within DVUA0055 at 67.803 kb on + strand, within DVUA0055 at 67.874 kb on + strand at 67.892 kb on - strand at 67.911 kb on + strand at 67.966 kb on + strand at 67.974 kb on - strand, within DVUA0056 at 68.040 kb on + strand, within DVUA0056 at 68.045 kb on + strand, within DVUA0056 at 68.045 kb on + strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.053 kb on - strand, within DVUA0056 at 68.143 kb on - strand, within DVUA0056 at 68.175 kb on + strand, within DVUA0056 at 68.181 kb on + strand, within DVUA0056 at 68.209 kb on - strand, within DVUA0056 at 68.212 kb on + strand, within DVUA0056 at 68.258 kb on - strand, within DVUA0056 at 68.427 kb on + strand, within DVUA0056 at 68.432 kb on + strand, within DVUA0056 at 68.450 kb on - strand, within DVUA0056 at 68.688 kb on + strand at 68.703 kb on - strand at 68.816 kb on - strand at 68.920 kb on + strand, within atoC
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 65,980 + DVUA0054 0.29 +0.1 66,012 + DVUA0054 0.31 -1.7 66,045 - DVUA0054 0.34 +1.7 66,055 - DVUA0054 0.34 -0.1 66,147 - DVUA0054 0.41 -1.3 66,149 + DVUA0054 0.41 +0.7 66,221 + DVUA0054 0.46 -1.6 66,292 + DVUA0054 0.51 -2.0 66,344 - DVUA0054 0.55 -1.2 66,401 - DVUA0054 0.59 -0.5 66,413 + DVUA0054 0.60 +0.1 66,464 - DVUA0054 0.64 -1.2 66,479 - DVUA0054 0.65 +1.0 66,487 - DVUA0054 0.65 -0.5 66,507 + DVUA0054 0.67 -1.8 66,521 + DVUA0054 0.68 +2.1 66,543 + DVUA0054 0.69 -0.6 66,574 - DVUA0054 0.72 +1.9 66,651 - DVUA0054 0.77 -2.6 66,743 + DVUA0054 0.84 +1.2 66,743 + DVUA0054 0.84 -1.6 66,751 - DVUA0054 0.84 -1.3 66,769 - DVUA0054 0.85 -0.7 66,907 + -0.7 66,921 + +2.1 67,007 - +1.9 67,061 + -2.9 67,102 + DVUA0055 0.14 -1.4 67,102 + DVUA0055 0.14 +2.1 67,105 + DVUA0055 0.14 -1.3 67,108 - DVUA0055 0.15 +1.1 67,117 + DVUA0055 0.16 -0.6 67,125 - DVUA0055 0.16 +0.1 67,137 + DVUA0055 0.18 -0.7 67,172 - DVUA0055 0.21 +2.3 67,231 + DVUA0055 0.27 +0.9 67,231 + DVUA0055 0.27 -0.0 67,239 - DVUA0055 0.28 -1.3 67,239 - DVUA0055 0.28 -0.8 67,275 + DVUA0055 0.32 -0.4 67,370 + DVUA0055 0.42 -0.1 67,370 + DVUA0055 0.42 +1.7 67,370 + DVUA0055 0.42 +1.5 67,370 + DVUA0055 0.42 -1.3 67,378 - DVUA0055 0.43 +4.1 67,378 - DVUA0055 0.43 +1.1 67,446 + DVUA0055 0.50 +3.1 67,651 + DVUA0055 0.71 +0.4 67,803 + DVUA0055 0.87 -0.7 67,803 + DVUA0055 0.87 -1.2 67,874 + +0.8 67,892 - +3.1 67,911 + -0.2 67,966 + -0.3 67,974 - DVUA0056 0.10 -0.7 68,040 + DVUA0056 0.21 -1.0 68,045 + DVUA0056 0.21 -0.7 68,045 + DVUA0056 0.21 -0.9 68,053 - DVUA0056 0.23 -1.3 68,053 - DVUA0056 0.23 -1.3 68,053 - DVUA0056 0.23 -2.1 68,053 - DVUA0056 0.23 -1.0 68,143 - DVUA0056 0.37 +1.5 68,175 + DVUA0056 0.42 -1.5 68,181 + DVUA0056 0.43 -1.9 68,209 - DVUA0056 0.48 +1.1 68,212 + DVUA0056 0.48 -1.1 68,258 - DVUA0056 0.56 +3.7 68,427 + DVUA0056 0.83 -1.0 68,432 + DVUA0056 0.84 -0.2 68,450 - DVUA0056 0.87 -1.7 68,688 + -0.2 68,703 - -0.4 68,816 - -1.3 68,920 + atoC DVUA0057 0.16 +2.1
Or see this region's nucleotide sequence