Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gmd and DVU0449 overlap by 13 nucleotides DVU0449 and tRNA-Leu are separated by 279 nucleotides tRNA-Leu and ribF are separated by 482 nucleotides
DVU0448: gmd - GDP-mannose 4,6-dehydratase (TIGR), at 508,293 to 509,438
gmd
DVU0449: DVU0449 - sensor/response regulator (TIGR), at 509,426 to 513,967
DVU0449
DVU_tRNA-Leu-2: tRNA-Leu - tRNA with anticodon CAG for Leu (VIMSS-AUTO), at 514,247 to 514,333
tRNA-Leu
DVU0450: ribF - riboflavin biosynthesis protein RibF (TIGR), at 514,816 to 515,760
ribF
Position (kb) 509
510
511
512
513
514 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 508.536 kb on + strand, within gmd at 508.544 kb on - strand, within gmd at 508.651 kb on + strand, within gmd at 508.826 kb on - strand, within gmd at 508.866 kb on + strand, within gmd at 508.957 kb on - strand, within gmd at 508.959 kb on + strand, within gmd at 509.062 kb on - strand, within gmd at 509.474 kb on + strand at 509.503 kb on - strand at 509.508 kb on - strand at 509.615 kb on - strand at 509.619 kb on + strand at 509.705 kb on + strand at 509.793 kb on - strand at 509.836 kb on + strand at 509.844 kb on - strand at 509.844 kb on - strand at 509.865 kb on + strand at 509.873 kb on - strand at 509.882 kb on - strand, within DVU0449 at 509.961 kb on - strand, within DVU0449 at 510.114 kb on + strand, within DVU0449 at 510.114 kb on + strand, within DVU0449 at 510.114 kb on + strand, within DVU0449 at 510.127 kb on - strand, within DVU0449 at 510.127 kb on - strand, within DVU0449 at 510.127 kb on - strand, within DVU0449 at 510.127 kb on - strand, within DVU0449 at 510.143 kb on + strand, within DVU0449 at 510.176 kb on + strand, within DVU0449 at 510.184 kb on - strand, within DVU0449 at 510.218 kb on - strand, within DVU0449 at 510.220 kb on - strand, within DVU0449 at 510.240 kb on + strand, within DVU0449 at 510.265 kb on - strand, within DVU0449 at 510.344 kb on - strand, within DVU0449 at 510.349 kb on + strand, within DVU0449 at 510.359 kb on + strand, within DVU0449 at 510.370 kb on - strand, within DVU0449 at 510.417 kb on + strand, within DVU0449 at 510.462 kb on + strand, within DVU0449 at 510.478 kb on + strand, within DVU0449 at 510.498 kb on + strand, within DVU0449 at 510.508 kb on + strand, within DVU0449 at 510.516 kb on - strand, within DVU0449 at 510.526 kb on - strand, within DVU0449 at 510.704 kb on + strand, within DVU0449 at 510.706 kb on + strand, within DVU0449 at 510.840 kb on + strand, within DVU0449 at 510.848 kb on - strand, within DVU0449 at 510.848 kb on - strand, within DVU0449 at 510.868 kb on + strand, within DVU0449 at 510.911 kb on - strand, within DVU0449 at 510.922 kb on + strand, within DVU0449 at 510.957 kb on - strand, within DVU0449 at 511.080 kb on + strand, within DVU0449 at 511.110 kb on - strand, within DVU0449 at 511.236 kb on + strand, within DVU0449 at 511.244 kb on - strand, within DVU0449 at 511.244 kb on - strand, within DVU0449 at 511.244 kb on - strand, within DVU0449 at 511.244 kb on - strand, within DVU0449 at 511.296 kb on + strand, within DVU0449 at 511.296 kb on + strand, within DVU0449 at 511.304 kb on - strand, within DVU0449 at 511.304 kb on - strand, within DVU0449 at 511.371 kb on - strand, within DVU0449 at 511.409 kb on - strand, within DVU0449 at 511.436 kb on + strand, within DVU0449 at 511.449 kb on + strand, within DVU0449 at 511.498 kb on - strand, within DVU0449 at 511.543 kb on + strand, within DVU0449 at 511.551 kb on - strand, within DVU0449 at 511.565 kb on - strand, within DVU0449 at 511.723 kb on + strand, within DVU0449 at 511.731 kb on - strand, within DVU0449 at 511.788 kb on + strand, within DVU0449 at 511.796 kb on - strand, within DVU0449 at 511.801 kb on - strand, within DVU0449 at 511.817 kb on - strand, within DVU0449 at 511.848 kb on + strand, within DVU0449 at 511.900 kb on - strand, within DVU0449 at 511.929 kb on - strand, within DVU0449 at 511.986 kb on - strand, within DVU0449 at 511.986 kb on - strand, within DVU0449 at 511.986 kb on - strand, within DVU0449 at 511.988 kb on - strand, within DVU0449 at 512.029 kb on - strand, within DVU0449 at 512.117 kb on + strand, within DVU0449 at 512.145 kb on + strand, within DVU0449 at 512.145 kb on + strand, within DVU0449 at 512.195 kb on + strand, within DVU0449 at 512.203 kb on - strand, within DVU0449 at 512.208 kb on - strand, within DVU0449 at 512.253 kb on + strand, within DVU0449 at 512.289 kb on + strand, within DVU0449 at 512.330 kb on - strand, within DVU0449 at 512.335 kb on + strand, within DVU0449 at 512.395 kb on - strand, within DVU0449 at 512.399 kb on + strand, within DVU0449 at 512.466 kb on - strand, within DVU0449 at 512.466 kb on - strand, within DVU0449 at 512.550 kb on + strand, within DVU0449 at 512.555 kb on + strand, within DVU0449 at 512.560 kb on + strand, within DVU0449 at 512.601 kb on + strand, within DVU0449 at 512.606 kb on + strand, within DVU0449 at 512.609 kb on - strand, within DVU0449 at 512.628 kb on - strand, within DVU0449 at 512.688 kb on + strand, within DVU0449 at 512.707 kb on + strand, within DVU0449 at 512.707 kb on + strand, within DVU0449 at 512.715 kb on - strand, within DVU0449 at 512.715 kb on - strand, within DVU0449 at 512.715 kb on - strand, within DVU0449 at 512.811 kb on + strand, within DVU0449 at 512.811 kb on + strand, within DVU0449 at 512.818 kb on + strand, within DVU0449 at 512.831 kb on - strand, within DVU0449 at 512.852 kb on + strand, within DVU0449 at 512.871 kb on + strand, within DVU0449 at 512.927 kb on + strand, within DVU0449 at 512.936 kb on - strand, within DVU0449 at 512.936 kb on - strand, within DVU0449 at 512.990 kb on - strand, within DVU0449 at 513.050 kb on - strand, within DVU0449 at 513.069 kb on + strand, within DVU0449 at 513.092 kb on + strand, within DVU0449 at 513.092 kb on + strand, within DVU0449 at 513.092 kb on + strand, within DVU0449 at 513.100 kb on - strand, within DVU0449 at 513.100 kb on - strand, within DVU0449 at 513.100 kb on - strand, within DVU0449 at 513.100 kb on - strand, within DVU0449 at 513.110 kb on - strand, within DVU0449 at 513.127 kb on + strand, within DVU0449 at 513.167 kb on - strand, within DVU0449 at 513.207 kb on - strand, within DVU0449 at 513.239 kb on - strand, within DVU0449 at 513.249 kb on - strand, within DVU0449 at 513.314 kb on + strand, within DVU0449 at 513.319 kb on + strand, within DVU0449 at 513.319 kb on + strand, within DVU0449 at 513.422 kb on + strand, within DVU0449 at 513.468 kb on - strand, within DVU0449 at 513.526 kb on + strand at 513.549 kb on - strand at 513.589 kb on - strand at 513.622 kb on + strand at 513.622 kb on + strand at 513.630 kb on - strand at 513.650 kb on - strand at 513.696 kb on + strand at 513.719 kb on + strand at 513.719 kb on + strand at 513.719 kb on + strand at 513.801 kb on + strand at 513.825 kb on - strand at 514.002 kb on + strand at 514.068 kb on + strand at 514.068 kb on + strand at 514.068 kb on + strand at 514.076 kb on - strand at 514.089 kb on + strand at 514.089 kb on + strand at 514.091 kb on - strand at 514.141 kb on - strand at 514.170 kb on + strand at 514.420 kb on - strand at 514.431 kb on - strand at 514.556 kb on - strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.561 kb on + strand at 514.569 kb on - strand at 514.569 kb on - strand at 514.569 kb on - strand at 514.576 kb on + strand at 514.584 kb on - strand at 514.585 kb on + strand at 514.592 kb on - strand at 514.631 kb on + strand at 514.653 kb on + strand at 514.694 kb on - strand at 514.710 kb on + strand at 514.739 kb on + strand at 514.749 kb on + strand at 514.802 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 508,536 + gmd DVU0448 0.21 +3.1 508,544 - gmd DVU0448 0.22 +4.7 508,651 + gmd DVU0448 0.31 +1.5 508,826 - gmd DVU0448 0.47 +2.7 508,866 + gmd DVU0448 0.50 +4.7 508,957 - gmd DVU0448 0.58 +1.5 508,959 + gmd DVU0448 0.58 +2.7 509,062 - gmd DVU0448 0.67 +4.7 509,474 + +0.4 509,503 - -1.3 509,508 - +0.9 509,615 - -1.8 509,619 + -1.0 509,705 + +0.4 509,793 - -0.9 509,836 + -1.3 509,844 - -0.7 509,844 - -1.5 509,865 + -1.4 509,873 - +3.7 509,882 - DVU0449 0.10 -0.8 509,961 - DVU0449 0.12 -0.3 510,114 + DVU0449 0.15 +1.5 510,114 + DVU0449 0.15 +0.4 510,114 + DVU0449 0.15 +1.7 510,127 - DVU0449 0.15 -0.5 510,127 - DVU0449 0.15 -0.9 510,127 - DVU0449 0.15 -3.2 510,127 - DVU0449 0.15 -1.5 510,143 + DVU0449 0.16 +2.4 510,176 + DVU0449 0.17 +0.7 510,184 - DVU0449 0.17 +2.4 510,218 - DVU0449 0.17 -1.6 510,220 - DVU0449 0.17 -2.5 510,240 + DVU0449 0.18 -1.0 510,265 - DVU0449 0.18 -0.2 510,344 - DVU0449 0.20 +1.1 510,349 + DVU0449 0.20 -0.7 510,359 + DVU0449 0.21 +0.8 510,370 - DVU0449 0.21 -0.4 510,417 + DVU0449 0.22 -1.5 510,462 + DVU0449 0.23 -1.8 510,478 + DVU0449 0.23 -1.9 510,498 + DVU0449 0.24 -1.6 510,508 + DVU0449 0.24 -1.3 510,516 - DVU0449 0.24 +0.8 510,526 - DVU0449 0.24 -1.8 510,704 + DVU0449 0.28 -0.5 510,706 + DVU0449 0.28 +0.8 510,840 + DVU0449 0.31 +1.0 510,848 - DVU0449 0.31 +3.7 510,848 - DVU0449 0.31 +1.7 510,868 + DVU0449 0.32 -1.2 510,911 - DVU0449 0.33 +1.1 510,922 + DVU0449 0.33 -1.2 510,957 - DVU0449 0.34 -1.4 511,080 + DVU0449 0.36 +1.9 511,110 - DVU0449 0.37 +2.4 511,236 + DVU0449 0.40 -0.1 511,244 - DVU0449 0.40 -1.6 511,244 - DVU0449 0.40 -0.4 511,244 - DVU0449 0.40 -1.9 511,244 - DVU0449 0.40 +2.1 511,296 + DVU0449 0.41 -1.0 511,296 + DVU0449 0.41 -0.9 511,304 - DVU0449 0.41 +2.1 511,304 - DVU0449 0.41 -1.8 511,371 - DVU0449 0.43 -1.0 511,409 - DVU0449 0.44 -1.7 511,436 + DVU0449 0.44 -0.3 511,449 + DVU0449 0.45 +0.5 511,498 - DVU0449 0.46 +1.1 511,543 + DVU0449 0.47 +0.7 511,551 - DVU0449 0.47 -2.6 511,565 - DVU0449 0.47 -1.6 511,723 + DVU0449 0.51 +0.7 511,731 - DVU0449 0.51 -0.2 511,788 + DVU0449 0.52 -1.6 511,796 - DVU0449 0.52 +1.1 511,801 - DVU0449 0.52 +0.8 511,817 - DVU0449 0.53 +1.5 511,848 + DVU0449 0.53 +0.9 511,900 - DVU0449 0.54 -2.4 511,929 - DVU0449 0.55 -0.7 511,986 - DVU0449 0.56 -0.1 511,986 - DVU0449 0.56 +2.1 511,986 - DVU0449 0.56 -1.5 511,988 - DVU0449 0.56 -1.3 512,029 - DVU0449 0.57 -1.5 512,117 + DVU0449 0.59 -1.1 512,145 + DVU0449 0.60 -0.7 512,145 + DVU0449 0.60 +0.5 512,195 + DVU0449 0.61 -2.3 512,203 - DVU0449 0.61 +2.4 512,208 - DVU0449 0.61 -1.7 512,253 + DVU0449 0.62 -1.5 512,289 + DVU0449 0.63 -0.6 512,330 - DVU0449 0.64 -0.9 512,335 + DVU0449 0.64 +1.1 512,395 - DVU0449 0.65 +3.7 512,399 + DVU0449 0.65 -1.3 512,466 - DVU0449 0.67 -0.9 512,466 - DVU0449 0.67 -2.4 512,550 + DVU0449 0.69 +1.1 512,555 + DVU0449 0.69 -0.8 512,560 + DVU0449 0.69 +0.1 512,601 + DVU0449 0.70 +2.4 512,606 + DVU0449 0.70 +0.0 512,609 - DVU0449 0.70 -2.0 512,628 - DVU0449 0.70 -1.3 512,688 + DVU0449 0.72 -0.4 512,707 + DVU0449 0.72 +0.4 512,707 + DVU0449 0.72 -1.7 512,715 - DVU0449 0.72 -1.2 512,715 - DVU0449 0.72 -2.1 512,715 - DVU0449 0.72 -1.7 512,811 + DVU0449 0.75 -1.2 512,811 + DVU0449 0.75 -0.8 512,818 + DVU0449 0.75 -1.4 512,831 - DVU0449 0.75 -1.1 512,852 + DVU0449 0.75 -0.8 512,871 + DVU0449 0.76 -1.1 512,927 + DVU0449 0.77 +1.5 512,936 - DVU0449 0.77 -1.7 512,936 - DVU0449 0.77 -0.6 512,990 - DVU0449 0.78 +0.8 513,050 - DVU0449 0.80 -1.2 513,069 + DVU0449 0.80 +1.7 513,092 + DVU0449 0.81 +0.4 513,092 + DVU0449 0.81 -1.5 513,092 + DVU0449 0.81 -0.9 513,100 - DVU0449 0.81 -2.2 513,100 - DVU0449 0.81 -1.7 513,100 - DVU0449 0.81 +1.1 513,100 - DVU0449 0.81 +1.0 513,110 - DVU0449 0.81 -2.1 513,127 + DVU0449 0.81 +1.1 513,167 - DVU0449 0.82 -1.1 513,207 - DVU0449 0.83 -2.0 513,239 - DVU0449 0.84 +1.5 513,249 - DVU0449 0.84 -0.1 513,314 + DVU0449 0.86 +1.0 513,319 + DVU0449 0.86 +4.7 513,319 + DVU0449 0.86 -0.3 513,422 + DVU0449 0.88 -1.0 513,468 - DVU0449 0.89 -0.3 513,526 + +3.1 513,549 - +0.8 513,589 - -1.0 513,622 + +2.7 513,622 + -0.5 513,630 - -2.5 513,650 - -0.1 513,696 + -1.2 513,719 + +0.3 513,719 + +1.4 513,719 + -1.0 513,801 + -0.8 513,825 - -2.2 514,002 + +1.2 514,068 + -0.4 514,068 + -1.0 514,068 + -1.1 514,076 - -0.9 514,089 + -0.9 514,089 + +0.8 514,091 - -0.5 514,141 - -1.4 514,170 + -1.5 514,420 - +3.7 514,431 - -0.3 514,556 - -1.8 514,561 + -0.6 514,561 + -1.9 514,561 + -2.2 514,561 + -0.9 514,569 - -1.4 514,569 - +0.8 514,569 - +3.7 514,576 + -0.9 514,584 - -1.8 514,585 + -1.8 514,592 - -0.6 514,631 + -0.3 514,653 + -0.7 514,694 - -1.5 514,710 + -1.2 514,739 + -0.3 514,749 + -1.4 514,802 - -1.6
Or see this region's nucleotide sequence