Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0445 and DVU0446 are separated by 148 nucleotides DVU0446 and DVU0447 overlap by 8 nucleotides DVU0447 and gmd are separated by 393 nucleotides
DVU0445: DVU0445 - CBS domain protein (TIGR), at 504,169 to 505,893
DVU0445
DVU0446: DVU0446 - sodium/solute symporter family protein (TIGR), at 506,042 to 507,589
DVU0446
DVU0447: DVU0447 - membrane protein, putative (TIGR), at 507,582 to 507,899
DVU0447
DVU0448: gmd - GDP-mannose 4,6-dehydratase (TIGR), at 508,293 to 509,438
gmd
Position (kb) 506
507
508 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2
3
4 at 505.156 kb on - strand, within DVU0445 at 505.176 kb on + strand, within DVU0445 at 505.195 kb on + strand, within DVU0445 at 505.230 kb on + strand, within DVU0445 at 505.240 kb on + strand, within DVU0445 at 505.267 kb on + strand, within DVU0445 at 505.334 kb on - strand, within DVU0445 at 505.363 kb on + strand, within DVU0445 at 505.371 kb on - strand, within DVU0445 at 505.492 kb on + strand, within DVU0445 at 505.519 kb on + strand, within DVU0445 at 505.527 kb on - strand, within DVU0445 at 505.565 kb on - strand, within DVU0445 at 505.585 kb on - strand, within DVU0445 at 505.615 kb on + strand, within DVU0445 at 505.627 kb on + strand, within DVU0445 at 505.724 kb on - strand at 505.754 kb on - strand at 505.777 kb on + strand at 505.846 kb on - strand at 505.897 kb on + strand at 505.995 kb on - strand at 506.039 kb on + strand at 506.109 kb on - strand at 506.207 kb on + strand, within DVU0446 at 506.207 kb on + strand, within DVU0446 at 506.215 kb on - strand, within DVU0446 at 506.216 kb on + strand, within DVU0446 at 506.276 kb on + strand, within DVU0446 at 506.284 kb on - strand, within DVU0446 at 506.284 kb on - strand, within DVU0446 at 506.284 kb on - strand, within DVU0446 at 506.338 kb on + strand, within DVU0446 at 506.346 kb on - strand, within DVU0446 at 506.406 kb on + strand, within DVU0446 at 506.475 kb on + strand, within DVU0446 at 506.475 kb on - strand, within DVU0446 at 506.490 kb on + strand, within DVU0446 at 506.543 kb on + strand, within DVU0446 at 506.551 kb on - strand, within DVU0446 at 506.558 kb on - strand, within DVU0446 at 506.693 kb on + strand, within DVU0446 at 506.736 kb on - strand, within DVU0446 at 506.796 kb on + strand, within DVU0446 at 506.804 kb on - strand, within DVU0446 at 506.804 kb on - strand, within DVU0446 at 506.804 kb on - strand, within DVU0446 at 506.848 kb on - strand, within DVU0446 at 506.887 kb on - strand, within DVU0446 at 507.205 kb on - strand, within DVU0446 at 507.209 kb on + strand, within DVU0446 at 507.237 kb on - strand, within DVU0446 at 507.330 kb on + strand, within DVU0446 at 507.415 kb on + strand, within DVU0446 at 507.446 kb on + strand at 507.451 kb on - strand at 507.477 kb on + strand at 507.535 kb on - strand at 507.560 kb on - strand at 507.560 kb on - strand at 507.562 kb on + strand at 507.562 kb on + strand at 507.570 kb on - strand at 507.570 kb on - strand at 507.570 kb on - strand at 507.607 kb on - strand at 507.685 kb on + strand, within DVU0447 at 507.719 kb on + strand, within DVU0447 at 507.782 kb on + strand, within DVU0447 at 507.800 kb on + strand, within DVU0447 at 507.811 kb on + strand, within DVU0447 at 507.819 kb on - strand, within DVU0447 at 507.863 kb on - strand, within DVU0447 at 507.898 kb on - strand at 507.907 kb on + strand at 507.915 kb on - strand at 507.917 kb on - strand at 507.998 kb on - strand at 507.998 kb on - strand at 508.016 kb on + strand at 508.024 kb on - strand at 508.024 kb on - strand at 508.048 kb on - strand at 508.053 kb on - strand at 508.080 kb on + strand at 508.088 kb on - strand at 508.374 kb on - strand at 508.536 kb on + strand, within gmd at 508.544 kb on - strand, within gmd
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 505,156 - DVU0445 0.57 -0.7 505,176 + DVU0445 0.58 -0.6 505,195 + DVU0445 0.59 +0.9 505,230 + DVU0445 0.62 -1.7 505,240 + DVU0445 0.62 -1.8 505,267 + DVU0445 0.64 -1.9 505,334 - DVU0445 0.68 -0.5 505,363 + DVU0445 0.69 -0.9 505,371 - DVU0445 0.70 -2.1 505,492 + DVU0445 0.77 +3.1 505,519 + DVU0445 0.78 +2.4 505,527 - DVU0445 0.79 -0.6 505,565 - DVU0445 0.81 -1.7 505,585 - DVU0445 0.82 -0.7 505,615 + DVU0445 0.84 -0.2 505,627 + DVU0445 0.85 -0.6 505,724 - -1.5 505,754 - +0.7 505,777 + -1.6 505,846 - +1.2 505,897 + -1.3 505,995 - -1.1 506,039 + -4.2 506,109 - -1.0 506,207 + DVU0446 0.11 +4.7 506,207 + DVU0446 0.11 -0.8 506,215 - DVU0446 0.11 -0.7 506,216 + DVU0446 0.11 -1.1 506,276 + DVU0446 0.15 +2.4 506,284 - DVU0446 0.16 -1.3 506,284 - DVU0446 0.16 +0.5 506,284 - DVU0446 0.16 -0.0 506,338 + DVU0446 0.19 -1.6 506,346 - DVU0446 0.20 -1.0 506,406 + DVU0446 0.24 -0.4 506,475 + DVU0446 0.28 -1.8 506,475 - DVU0446 0.28 -2.6 506,490 + DVU0446 0.29 -1.3 506,543 + DVU0446 0.32 -1.4 506,551 - DVU0446 0.33 +2.1 506,558 - DVU0446 0.33 -0.2 506,693 + DVU0446 0.42 +2.9 506,736 - DVU0446 0.45 -1.7 506,796 + DVU0446 0.49 -0.9 506,804 - DVU0446 0.49 +0.8 506,804 - DVU0446 0.49 -0.6 506,804 - DVU0446 0.49 +1.7 506,848 - DVU0446 0.52 +0.8 506,887 - DVU0446 0.55 -2.2 507,205 - DVU0446 0.75 -0.3 507,209 + DVU0446 0.75 +0.6 507,237 - DVU0446 0.77 -0.7 507,330 + DVU0446 0.83 +1.2 507,415 + DVU0446 0.89 -1.5 507,446 + -0.6 507,451 - +1.4 507,477 + +0.3 507,535 - +1.1 507,560 - +3.4 507,560 - -0.5 507,562 + -1.0 507,562 + -1.5 507,570 - -0.6 507,570 - -2.4 507,570 - +0.4 507,607 - -1.5 507,685 + DVU0447 0.32 -3.5 507,719 + DVU0447 0.43 +0.9 507,782 + DVU0447 0.63 +0.7 507,800 + DVU0447 0.69 -1.6 507,811 + DVU0447 0.72 +1.4 507,819 - DVU0447 0.75 +1.1 507,863 - DVU0447 0.88 -2.5 507,898 - -1.5 507,907 + +0.5 507,915 - +3.1 507,917 - +0.6 507,998 - -1.6 507,998 - -1.4 508,016 + +2.1 508,024 - -1.1 508,024 - -1.2 508,048 - -0.6 508,053 - +1.9 508,080 + -1.1 508,088 - -1.1 508,374 - +4.7 508,536 + gmd DVU0448 0.21 +3.1 508,544 - gmd DVU0448 0.22 +4.7
Or see this region's nucleotide sequence