Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0416 and DORF27863 are separated by 336 nucleotides DORF27863 and speA are separated by 60 nucleotides speA and lys1 are separated by 248 nucleotides
DVU0416: DVU0416 - GGDEF domain protein (TIGR), at 467,022 to 468,140
DVU0416
DORF27863: DORF27863 - no description, at 468,477 to 468,596
DORF27863
DVU0417: speA - arginine decarboxylase (TIGR), at 468,657 to 470,636
speA
DVU0418: lys1 - saccharopine dehydrogenase (TIGR), at 470,885 to 472,075
lys1
Position (kb) 468
469
470
471 Strain fitness (log2 ratio) -3
-2
-1
0
1
2
3
4 at 467.705 kb on + strand, within DVU0416 at 467.705 kb on + strand, within DVU0416 at 467.713 kb on - strand, within DVU0416 at 467.728 kb on - strand, within DVU0416 at 467.749 kb on + strand, within DVU0416 at 467.782 kb on + strand, within DVU0416 at 467.782 kb on + strand, within DVU0416 at 467.811 kb on + strand, within DVU0416 at 467.820 kb on + strand, within DVU0416 at 467.970 kb on + strand, within DVU0416 at 467.975 kb on - strand, within DVU0416 at 468.051 kb on - strand at 468.072 kb on + strand at 468.072 kb on + strand at 468.080 kb on - strand at 468.080 kb on - strand at 468.111 kb on + strand at 468.112 kb on - strand at 468.131 kb on - strand at 468.140 kb on + strand at 468.145 kb on - strand at 468.404 kb on - strand at 468.497 kb on - strand, within DORF27863 at 468.497 kb on - strand, within DORF27863 at 468.511 kb on + strand, within DORF27863 at 468.545 kb on + strand, within DORF27863 at 468.606 kb on + strand at 468.606 kb on + strand at 468.606 kb on + strand at 468.606 kb on + strand at 468.606 kb on + strand at 468.614 kb on - strand at 468.614 kb on - strand at 468.637 kb on + strand at 468.716 kb on - strand at 468.740 kb on + strand at 468.750 kb on + strand at 468.760 kb on + strand at 468.765 kb on + strand at 468.767 kb on + strand at 468.767 kb on + strand at 468.775 kb on - strand at 468.775 kb on - strand at 468.843 kb on + strand at 468.912 kb on - strand, within speA at 468.948 kb on - strand, within speA at 469.008 kb on + strand, within speA at 469.016 kb on - strand, within speA at 469.067 kb on - strand, within speA at 469.253 kb on + strand, within speA at 469.267 kb on + strand, within speA at 469.303 kb on - strand, within speA at 469.360 kb on + strand, within speA at 469.368 kb on - strand, within speA at 469.429 kb on + strand, within speA at 469.450 kb on - strand, within speA at 469.491 kb on + strand, within speA at 469.559 kb on + strand, within speA at 469.587 kb on - strand, within speA at 469.608 kb on + strand, within speA at 469.699 kb on + strand, within speA at 469.734 kb on - strand, within speA at 469.835 kb on + strand, within speA at 469.854 kb on - strand, within speA at 469.908 kb on - strand, within speA at 469.910 kb on - strand, within speA at 469.983 kb on - strand, within speA at 470.014 kb on - strand, within speA at 470.056 kb on - strand, within speA at 470.121 kb on - strand, within speA at 470.258 kb on + strand, within speA at 470.360 kb on - strand, within speA at 470.373 kb on + strand, within speA at 470.490 kb on - strand at 470.506 kb on + strand at 470.586 kb on + strand at 470.614 kb on + strand at 470.618 kb on + strand at 470.618 kb on + strand at 470.624 kb on + strand at 470.732 kb on - strand at 470.732 kb on - strand at 470.844 kb on + strand at 470.856 kb on + strand at 470.950 kb on + strand at 471.046 kb on + strand, within lys1 at 471.046 kb on + strand, within lys1 at 471.056 kb on + strand, within lys1 at 471.129 kb on + strand, within lys1 at 471.129 kb on + strand, within lys1 at 471.130 kb on - strand, within lys1 at 471.157 kb on - strand, within lys1 at 471.176 kb on - strand, within lys1 at 471.187 kb on + strand, within lys1 at 471.216 kb on - strand, within lys1 at 471.234 kb on - strand, within lys1 at 471.504 kb on - strand, within lys1 at 471.584 kb on + strand, within lys1
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 467,705 + DVU0416 0.61 +0.5 467,705 + DVU0416 0.61 +3.3 467,713 - DVU0416 0.62 -1.0 467,728 - DVU0416 0.63 -1.2 467,749 + DVU0416 0.65 -1.2 467,782 + DVU0416 0.68 -1.6 467,782 + DVU0416 0.68 +3.7 467,811 + DVU0416 0.71 +0.3 467,820 + DVU0416 0.71 +0.6 467,970 + DVU0416 0.85 -0.6 467,975 - DVU0416 0.85 +2.2 468,051 - -1.8 468,072 + -1.0 468,072 + -0.5 468,080 - -1.8 468,080 - -0.4 468,111 + -1.0 468,112 - +0.9 468,131 - +2.3 468,140 + -2.2 468,145 - +2.7 468,404 - -0.9 468,497 - DORF27863 0.17 -2.1 468,497 - DORF27863 0.17 -1.9 468,511 + DORF27863 0.28 -2.9 468,545 + DORF27863 0.57 -1.5 468,606 + -0.9 468,606 + -0.7 468,606 + -2.1 468,606 + -1.0 468,606 + +3.1 468,614 - -0.4 468,614 - +1.0 468,637 + -0.7 468,716 - +0.4 468,740 + -1.1 468,750 + +3.1 468,760 + -3.6 468,765 + +0.5 468,767 + -2.4 468,767 + -1.5 468,775 - -1.4 468,775 - -0.9 468,843 + -2.0 468,912 - speA DVU0417 0.13 -1.9 468,948 - speA DVU0417 0.15 +3.1 469,008 + speA DVU0417 0.18 -2.5 469,016 - speA DVU0417 0.18 -2.4 469,067 - speA DVU0417 0.21 -1.4 469,253 + speA DVU0417 0.30 -1.3 469,267 + speA DVU0417 0.31 -2.2 469,303 - speA DVU0417 0.33 +2.1 469,360 + speA DVU0417 0.36 +1.5 469,368 - speA DVU0417 0.36 -2.7 469,429 + speA DVU0417 0.39 +0.1 469,450 - speA DVU0417 0.40 +3.7 469,491 + speA DVU0417 0.42 -1.5 469,559 + speA DVU0417 0.46 -3.1 469,587 - speA DVU0417 0.47 -2.6 469,608 + speA DVU0417 0.48 +0.6 469,699 + speA DVU0417 0.53 +4.7 469,734 - speA DVU0417 0.54 -2.7 469,835 + speA DVU0417 0.59 -0.7 469,854 - speA DVU0417 0.60 -1.1 469,908 - speA DVU0417 0.63 +2.3 469,910 - speA DVU0417 0.63 +0.0 469,983 - speA DVU0417 0.67 +2.3 470,014 - speA DVU0417 0.69 +3.7 470,056 - speA DVU0417 0.71 +0.9 470,121 - speA DVU0417 0.74 -1.4 470,258 + speA DVU0417 0.81 +1.9 470,360 - speA DVU0417 0.86 -0.3 470,373 + speA DVU0417 0.87 -0.9 470,490 - -2.6 470,506 + +3.7 470,586 + -1.5 470,614 + -2.7 470,618 + -3.1 470,618 + -0.8 470,624 + +1.3 470,732 - +0.6 470,732 - -1.3 470,844 + -0.6 470,856 + +0.7 470,950 + -2.8 471,046 + lys1 DVU0418 0.14 -2.6 471,046 + lys1 DVU0418 0.14 +1.0 471,056 + lys1 DVU0418 0.14 -0.6 471,129 + lys1 DVU0418 0.20 +3.1 471,129 + lys1 DVU0418 0.20 -2.9 471,130 - lys1 DVU0418 0.21 +1.9 471,157 - lys1 DVU0418 0.23 -1.7 471,176 - lys1 DVU0418 0.24 +1.1 471,187 + lys1 DVU0418 0.25 +3.1 471,216 - lys1 DVU0418 0.28 +0.0 471,234 - lys1 DVU0418 0.29 +1.2 471,504 - lys1 DVU0418 0.52 -3.3 471,584 + lys1 DVU0418 0.59 -0.6
Or see this region's nucleotide sequence