Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU0025 and tRNA-Ala are separated by 173 nucleotides tRNA-Ala and DVU0026 are separated by 364 nucleotides DVU0026 and DVU0027 are separated by 218 nucleotides
DVU0025: DVU0025 - sensory box histidine kinase (TIGR), at 27,714 to 29,873
DVU0025
DVU_tRNA-Ala-2: tRNA-Ala - tRNA with anticodon GGC for Ala (VIMSS-AUTO), at 30,047 to 30,122
tRNA-Ala
DVU0026: DVU0026 - conserved hypothetical protein (TIGR), at 30,487 to 31,059
DVU0026
DVU0027: DVU0027 - membrane protein, putative (TIGR), at 31,278 to 32,702
DVU0027
Position (kb)
30
31
32 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 29.497 kb on + strand, within DVU0025 at 29.546 kb on + strand, within DVU0025 at 29.569 kb on - strand, within DVU0025 at 29.571 kb on + strand, within DVU0025 at 29.579 kb on + strand, within DVU0025 at 29.596 kb on + strand, within DVU0025 at 29.676 kb on + strand at 29.702 kb on + strand at 29.722 kb on - strand at 29.758 kb on - strand at 29.778 kb on - strand at 29.789 kb on + strand at 29.866 kb on - strand at 29.906 kb on - strand at 29.933 kb on - strand at 29.940 kb on + strand at 29.952 kb on - strand at 30.091 kb on + strand, within tRNA-Ala at 30.227 kb on + strand at 30.240 kb on - strand at 30.305 kb on + strand at 30.366 kb on - strand at 30.379 kb on + strand at 30.485 kb on - strand at 30.708 kb on + strand, within DVU0026 at 30.763 kb on - strand, within DVU0026 at 30.809 kb on - strand, within DVU0026 at 30.818 kb on + strand, within DVU0026 at 30.833 kb on + strand, within DVU0026 at 30.849 kb on - strand, within DVU0026 at 30.909 kb on - strand, within DVU0026 at 30.938 kb on + strand, within DVU0026 at 30.938 kb on + strand, within DVU0026 at 31.008 kb on + strand at 31.052 kb on - strand at 31.080 kb on + strand at 31.252 kb on + strand at 31.364 kb on - strand at 31.371 kb on + strand at 31.374 kb on - strand at 31.437 kb on + strand, within DVU0027 at 31.532 kb on - strand, within DVU0027 at 31.591 kb on - strand, within DVU0027 at 31.623 kb on + strand, within DVU0027 at 31.660 kb on - strand, within DVU0027 at 31.832 kb on - strand, within DVU0027 at 31.832 kb on - strand, within DVU0027 at 31.993 kb on + strand, within DVU0027 at 31.993 kb on + strand, within DVU0027 at 31.998 kb on + strand, within DVU0027 at 32.044 kb on - strand, within DVU0027
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 29,497 + DVU0025 0.83 -1.3 29,546 + DVU0025 0.85 -0.9 29,569 - DVU0025 0.86 -1.2 29,571 + DVU0025 0.86 -0.9 29,579 + DVU0025 0.86 -1.2 29,596 + DVU0025 0.87 -0.7 29,676 + -0.9 29,702 + -1.3 29,722 - +2.6 29,758 - -0.5 29,778 - -2.3 29,789 + +1.5 29,866 - -2.3 29,906 - -1.2 29,933 - -2.6 29,940 + -0.3 29,952 - -1.9 30,091 + tRNA-Ala DVU_tRNA-Ala-2 0.58 +3.6 30,227 + -1.5 30,240 - +2.1 30,305 + +0.2 30,366 - +1.8 30,379 + -2.4 30,485 - +2.6 30,708 + DVU0026 0.39 -1.0 30,763 - DVU0026 0.48 +1.6 30,809 - DVU0026 0.56 +0.4 30,818 + DVU0026 0.58 -1.0 30,833 + DVU0026 0.60 -1.6 30,849 - DVU0026 0.63 +0.7 30,909 - DVU0026 0.74 -0.6 30,938 + DVU0026 0.79 -0.3 30,938 + DVU0026 0.79 +2.1 31,008 + -0.4 31,052 - -1.2 31,080 + -0.2 31,252 + -1.5 31,364 - -1.3 31,371 + -1.6 31,374 - -0.1 31,437 + DVU0027 0.11 +2.3 31,532 - DVU0027 0.18 -2.1 31,591 - DVU0027 0.22 -1.9 31,623 + DVU0027 0.24 -1.2 31,660 - DVU0027 0.27 -1.5 31,832 - DVU0027 0.39 -1.1 31,832 - DVU0027 0.39 -1.6 31,993 + DVU0027 0.50 +3.1 31,993 + DVU0027 0.50 -1.7 31,998 + DVU0027 0.51 -0.1 32,044 - DVU0027 0.54 +1.8
Or see this region's nucleotide sequence