Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thrS and tRNA-Val are separated by 85 nucleotides tRNA-Val and DVU2539 are separated by 24 nucleotides DVU2539 and DVU2540 are separated by 5 nucleotides
DVU2538: thrS - threonyl-tRNA synthetase (TIGR), at 2,649,245 to 2,651,179
thrS
DVU_tRNA-Val-2: tRNA-Val - tRNA with anticodon GAC for Val (VIMSS-AUTO), at 2,651,265 to 2,651,339
tRNA-Val
DVU2539: DVU2539 - hypothetical protein (TIGR), at 2,651,364 to 2,651,486
DVU2539
DVU2540: DVU2540 - lyase, putative (TIGR), at 2,651,492 to 2,652,634
DVU2540
Position (kb)
2651
2652 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2651.462 kb on + strand, within DVU2539 at 2651.479 kb on + strand at 2651.529 kb on + strand at 2651.551 kb on + strand at 2651.559 kb on - strand at 2651.595 kb on - strand at 2651.611 kb on + strand, within DVU2540 at 2651.617 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.634 kb on - strand, within DVU2540 at 2651.720 kb on - strand, within DVU2540 at 2651.732 kb on - strand, within DVU2540 at 2651.742 kb on + strand, within DVU2540 at 2651.778 kb on + strand, within DVU2540 at 2651.811 kb on - strand, within DVU2540 at 2651.829 kb on - strand, within DVU2540 at 2651.843 kb on + strand, within DVU2540 at 2651.870 kb on - strand, within DVU2540 at 2651.920 kb on - strand, within DVU2540 at 2651.946 kb on - strand, within DVU2540 at 2651.948 kb on - strand, within DVU2540 at 2651.957 kb on + strand, within DVU2540 at 2652.009 kb on - strand, within DVU2540 at 2652.091 kb on - strand, within DVU2540 at 2652.139 kb on - strand, within DVU2540 at 2652.165 kb on - strand, within DVU2540 at 2652.186 kb on - strand, within DVU2540 at 2652.202 kb on + strand, within DVU2540 at 2652.225 kb on + strand, within DVU2540 at 2652.225 kb on + strand, within DVU2540 at 2652.241 kb on + strand, within DVU2540 at 2652.283 kb on - strand, within DVU2540
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,651,462 + DVU2539 0.80 -1.9 2,651,479 + -0.8 2,651,529 + -1.3 2,651,551 + -2.0 2,651,559 - -0.8 2,651,595 - -0.1 2,651,611 + DVU2540 0.10 -1.5 2,651,617 - DVU2540 0.11 +0.9 2,651,634 - DVU2540 0.12 -1.5 2,651,634 - DVU2540 0.12 +1.5 2,651,720 - DVU2540 0.20 -1.8 2,651,732 - DVU2540 0.21 +4.7 2,651,742 + DVU2540 0.22 -1.8 2,651,778 + DVU2540 0.25 +1.9 2,651,811 - DVU2540 0.28 -0.2 2,651,829 - DVU2540 0.29 -1.8 2,651,843 + DVU2540 0.31 +1.1 2,651,870 - DVU2540 0.33 -0.8 2,651,920 - DVU2540 0.37 -1.5 2,651,946 - DVU2540 0.40 +2.1 2,651,948 - DVU2540 0.40 -0.2 2,651,957 + DVU2540 0.41 +1.9 2,652,009 - DVU2540 0.45 -1.7 2,652,091 - DVU2540 0.52 -0.9 2,652,139 - DVU2540 0.57 +1.5 2,652,165 - DVU2540 0.59 +3.7 2,652,186 - DVU2540 0.61 +0.1 2,652,202 + DVU2540 0.62 -1.3 2,652,225 + DVU2540 0.64 +0.7 2,652,225 + DVU2540 0.64 -2.1 2,652,241 + DVU2540 0.66 +1.4 2,652,283 - DVU2540 0.69 +0.4
Or see this region's nucleotide sequence