Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2131 and DVU2132 are separated by 266 nucleotides DVU2132 and DVU2133 are separated by 54 nucleotides DVU2133 and DVU2134 are separated by 134 nucleotides DVU2134 and DVU2135 are separated by 456 nucleotides
DVU2131: DVU2131 - hypothetical protein (TIGR), at 2,227,823 to 2,228,302
DVU2131
DVU2132: DVU2132 - hypothetical protein (TIGR), at 2,228,569 to 2,228,826
DVU2132
DVU2133: DVU2133 - membrane protein, putative (TIGR), at 2,228,881 to 2,230,620
DVU2133
DVU2134: DVU2134 - hypothetical protein (TIGR), at 2,230,755 to 2,230,883
DVU2134
DVU2135: DVU2135 - hypothetical protein (TIGR), at 2,231,340 to 2,231,891
DVU2135
Position (kb)
2228
2229
2230
2231 Strain fitness (log2 ratio)
-2
-1
0
1
2
3
4 at 2227.944 kb on + strand, within DVU2131 at 2227.959 kb on + strand, within DVU2131 at 2227.961 kb on + strand, within DVU2131 at 2227.969 kb on - strand, within DVU2131 at 2227.983 kb on + strand, within DVU2131 at 2227.985 kb on + strand, within DVU2131 at 2227.998 kb on - strand, within DVU2131 at 2227.998 kb on - strand, within DVU2131 at 2228.084 kb on - strand, within DVU2131 at 2228.091 kb on - strand, within DVU2131 at 2228.091 kb on - strand, within DVU2131 at 2228.213 kb on + strand, within DVU2131 at 2228.239 kb on + strand, within DVU2131 at 2228.239 kb on + strand, within DVU2131 at 2228.247 kb on - strand, within DVU2131 at 2228.247 kb on - strand, within DVU2131 at 2228.274 kb on - strand at 2228.382 kb on - strand at 2228.459 kb on - strand at 2228.500 kb on + strand at 2228.645 kb on - strand, within DVU2132 at 2228.778 kb on + strand, within DVU2132 at 2228.786 kb on - strand, within DVU2132 at 2228.792 kb on - strand, within DVU2132 at 2228.810 kb on + strand at 2228.823 kb on - strand at 2228.917 kb on + strand at 2228.952 kb on + strand at 2228.973 kb on + strand at 2229.037 kb on + strand at 2229.102 kb on - strand, within DVU2133 at 2229.112 kb on - strand, within DVU2133 at 2229.210 kb on + strand, within DVU2133 at 2229.237 kb on + strand, within DVU2133 at 2229.240 kb on - strand, within DVU2133 at 2229.245 kb on - strand, within DVU2133 at 2229.276 kb on - strand, within DVU2133 at 2229.451 kb on - strand, within DVU2133 at 2229.566 kb on - strand, within DVU2133 at 2229.732 kb on + strand, within DVU2133 at 2229.755 kb on - strand, within DVU2133 at 2229.815 kb on + strand, within DVU2133 at 2229.853 kb on - strand, within DVU2133 at 2229.873 kb on + strand, within DVU2133 at 2229.873 kb on + strand, within DVU2133 at 2229.931 kb on + strand, within DVU2133 at 2229.937 kb on - strand, within DVU2133 at 2229.994 kb on - strand, within DVU2133 at 2230.021 kb on - strand, within DVU2133 at 2230.078 kb on - strand, within DVU2133 at 2230.144 kb on - strand, within DVU2133 at 2230.204 kb on - strand, within DVU2133 at 2230.210 kb on - strand, within DVU2133 at 2230.311 kb on - strand, within DVU2133 at 2230.351 kb on + strand, within DVU2133 at 2230.403 kb on + strand, within DVU2133 at 2230.404 kb on - strand, within DVU2133 at 2230.411 kb on - strand, within DVU2133 at 2230.413 kb on - strand, within DVU2133 at 2230.450 kb on + strand at 2230.450 kb on - strand at 2230.450 kb on - strand at 2230.450 kb on - strand at 2230.567 kb on + strand at 2230.633 kb on + strand at 2231.007 kb on + strand at 2231.032 kb on + strand at 2231.032 kb on + strand at 2231.064 kb on - strand at 2231.072 kb on - strand at 2231.194 kb on - strand at 2231.243 kb on + strand at 2231.324 kb on + strand at 2231.383 kb on - strand at 2231.437 kb on + strand, within DVU2135 at 2231.444 kb on + strand, within DVU2135 at 2231.444 kb on + strand, within DVU2135 at 2231.452 kb on - strand, within DVU2135 at 2231.556 kb on + strand, within DVU2135 at 2231.583 kb on - strand, within DVU2135 at 2231.599 kb on - strand, within DVU2135 at 2231.605 kb on - strand, within DVU2135
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,227,944 + DVU2131 0.25 -2.6 2,227,959 + DVU2131 0.28 +2.5 2,227,961 + DVU2131 0.29 -0.4 2,227,969 - DVU2131 0.30 +3.8 2,227,983 + DVU2131 0.33 -0.8 2,227,985 + DVU2131 0.34 -1.3 2,227,998 - DVU2131 0.36 -0.6 2,227,998 - DVU2131 0.36 +1.0 2,228,084 - DVU2131 0.54 -1.3 2,228,091 - DVU2131 0.56 -0.7 2,228,091 - DVU2131 0.56 -0.7 2,228,213 + DVU2131 0.81 -1.6 2,228,239 + DVU2131 0.87 -1.9 2,228,239 + DVU2131 0.87 -0.3 2,228,247 - DVU2131 0.88 -1.3 2,228,247 - DVU2131 0.88 +0.6 2,228,274 - +1.2 2,228,382 - +0.8 2,228,459 - +0.5 2,228,500 + -0.2 2,228,645 - DVU2132 0.29 -1.9 2,228,778 + DVU2132 0.81 +1.2 2,228,786 - DVU2132 0.84 -1.4 2,228,792 - DVU2132 0.86 +2.2 2,228,810 + -1.9 2,228,823 - -0.9 2,228,917 + -1.2 2,228,952 + -1.1 2,228,973 + -1.9 2,229,037 + +3.8 2,229,102 - DVU2133 0.13 +2.1 2,229,112 - DVU2133 0.13 -0.6 2,229,210 + DVU2133 0.19 -1.1 2,229,237 + DVU2133 0.20 -0.7 2,229,240 - DVU2133 0.21 -1.2 2,229,245 - DVU2133 0.21 -1.4 2,229,276 - DVU2133 0.23 +1.0 2,229,451 - DVU2133 0.33 -2.3 2,229,566 - DVU2133 0.39 +1.8 2,229,732 + DVU2133 0.49 -1.3 2,229,755 - DVU2133 0.50 +2.8 2,229,815 + DVU2133 0.54 -2.1 2,229,853 - DVU2133 0.56 +1.6 2,229,873 + DVU2133 0.57 -1.1 2,229,873 + DVU2133 0.57 +1.2 2,229,931 + DVU2133 0.60 +0.9 2,229,937 - DVU2133 0.61 +2.5 2,229,994 - DVU2133 0.64 +2.8 2,230,021 - DVU2133 0.66 -0.5 2,230,078 - DVU2133 0.69 -1.4 2,230,144 - DVU2133 0.73 +0.1 2,230,204 - DVU2133 0.76 +2.0 2,230,210 - DVU2133 0.76 +0.6 2,230,311 - DVU2133 0.82 -0.2 2,230,351 + DVU2133 0.84 -0.6 2,230,403 + DVU2133 0.87 -0.1 2,230,404 - DVU2133 0.88 -1.8 2,230,411 - DVU2133 0.88 +0.8 2,230,413 - DVU2133 0.88 +0.9 2,230,450 + -0.3 2,230,450 - +0.3 2,230,450 - +1.5 2,230,450 - -0.5 2,230,567 + -1.6 2,230,633 + -1.5 2,231,007 + -2.0 2,231,032 + -0.6 2,231,032 + +0.9 2,231,064 - +2.5 2,231,072 - +0.3 2,231,194 - +0.8 2,231,243 + -0.9 2,231,324 + -0.4 2,231,383 - -1.3 2,231,437 + DVU2135 0.18 +2.6 2,231,444 + DVU2135 0.19 -1.7 2,231,444 + DVU2135 0.19 +0.3 2,231,452 - DVU2135 0.20 -1.7 2,231,556 + DVU2135 0.39 -0.9 2,231,583 - DVU2135 0.44 +1.5 2,231,599 - DVU2135 0.47 -1.0 2,231,605 - DVU2135 0.48 -1.6
Or see this region's nucleotide sequence