Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cpaC and DVU2120 are separated by 9 nucleotides DVU2120 and DVU2121 are separated by 11 nucleotides DVU2121 and cpaF are separated by 396 nucleotides
DVU2119: cpaC - type II/III secretion system protein (TIGR), at 2,213,886 to 2,215,340
cpaC
DVU2120: DVU2120 - hypothetical protein (TIGR), at 2,215,350 to 2,215,754
DVU2120
DVU2121: DVU2121 - response regulator (TIGR), at 2,215,766 to 2,216,980
DVU2121
DVU2122: cpaF - type II/IV secretion system protein (TIGR), at 2,217,377 to 2,218,810
cpaF
Position (kb)
2215
2216
2217 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4
5 at 2214.831 kb on + strand, within cpaC at 2214.853 kb on + strand, within cpaC at 2214.861 kb on - strand, within cpaC at 2214.865 kb on + strand, within cpaC at 2214.887 kb on - strand, within cpaC at 2214.892 kb on - strand, within cpaC at 2214.900 kb on + strand, within cpaC at 2214.906 kb on + strand, within cpaC at 2214.906 kb on + strand, within cpaC at 2214.911 kb on + strand, within cpaC at 2215.033 kb on + strand, within cpaC at 2215.038 kb on + strand, within cpaC at 2215.156 kb on + strand, within cpaC at 2215.227 kb on - strand at 2215.237 kb on - strand at 2215.436 kb on + strand, within DVU2120 at 2215.441 kb on + strand, within DVU2120 at 2215.444 kb on - strand, within DVU2120 at 2215.447 kb on - strand, within DVU2120 at 2215.449 kb on - strand, within DVU2120 at 2215.736 kb on + strand at 2216.154 kb on - strand, within DVU2121 at 2216.255 kb on + strand, within DVU2121 at 2216.257 kb on + strand, within DVU2121 at 2216.300 kb on + strand, within DVU2121 at 2216.412 kb on - strand, within DVU2121 at 2216.462 kb on + strand, within DVU2121 at 2216.500 kb on + strand, within DVU2121 at 2216.582 kb on + strand, within DVU2121 at 2216.653 kb on - strand, within DVU2121 at 2216.666 kb on + strand, within DVU2121 at 2216.685 kb on - strand, within DVU2121 at 2216.737 kb on + strand, within DVU2121 at 2216.737 kb on + strand, within DVU2121 at 2216.800 kb on + strand, within DVU2121 at 2216.808 kb on - strand, within DVU2121 at 2216.930 kb on + strand at 2216.974 kb on + strand at 2217.112 kb on + strand at 2217.256 kb on - strand at 2217.335 kb on + strand at 2217.424 kb on - strand at 2217.485 kb on + strand at 2217.495 kb on + strand at 2217.524 kb on - strand, within cpaF at 2217.539 kb on - strand, within cpaF at 2217.692 kb on - strand, within cpaF at 2217.764 kb on - strand, within cpaF at 2217.787 kb on - strand, within cpaF at 2217.859 kb on + strand, within cpaF at 2217.882 kb on + strand, within cpaF at 2217.900 kb on - strand, within cpaF at 2217.922 kb on - strand, within cpaF at 2217.960 kb on + strand, within cpaF at 2217.963 kb on - strand, within cpaF
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,214,831 + cpaC DVU2119 0.65 -0.5 2,214,853 + cpaC DVU2119 0.66 -1.2 2,214,861 - cpaC DVU2119 0.67 +4.1 2,214,865 + cpaC DVU2119 0.67 -0.9 2,214,887 - cpaC DVU2119 0.69 -1.8 2,214,892 - cpaC DVU2119 0.69 -1.1 2,214,900 + cpaC DVU2119 0.70 -0.1 2,214,906 + cpaC DVU2119 0.70 -0.2 2,214,906 + cpaC DVU2119 0.70 -0.9 2,214,911 + cpaC DVU2119 0.70 -1.4 2,215,033 + cpaC DVU2119 0.79 -0.9 2,215,038 + cpaC DVU2119 0.79 -0.1 2,215,156 + cpaC DVU2119 0.87 +0.5 2,215,227 - -3.2 2,215,237 - +2.5 2,215,436 + DVU2120 0.21 +0.6 2,215,441 + DVU2120 0.22 -2.1 2,215,444 - DVU2120 0.23 +2.2 2,215,447 - DVU2120 0.24 -0.3 2,215,449 - DVU2120 0.24 +2.8 2,215,736 + -0.8 2,216,154 - DVU2121 0.32 -2.7 2,216,255 + DVU2121 0.40 -3.0 2,216,257 + DVU2121 0.40 -1.5 2,216,300 + DVU2121 0.44 +2.8 2,216,412 - DVU2121 0.53 +1.8 2,216,462 + DVU2121 0.57 +0.0 2,216,500 + DVU2121 0.60 -3.0 2,216,582 + DVU2121 0.67 -0.6 2,216,653 - DVU2121 0.73 -1.7 2,216,666 + DVU2121 0.74 -1.8 2,216,685 - DVU2121 0.76 -0.6 2,216,737 + DVU2121 0.80 +1.5 2,216,737 + DVU2121 0.80 +0.3 2,216,800 + DVU2121 0.85 -2.3 2,216,808 - DVU2121 0.86 -0.7 2,216,930 + +1.5 2,216,974 + -1.7 2,217,112 + -1.7 2,217,256 - +3.8 2,217,335 + -1.5 2,217,424 - -1.0 2,217,485 + +0.8 2,217,495 + -1.8 2,217,524 - cpaF DVU2122 0.10 +1.8 2,217,539 - cpaF DVU2122 0.11 +1.8 2,217,692 - cpaF DVU2122 0.22 -0.8 2,217,764 - cpaF DVU2122 0.27 -1.1 2,217,787 - cpaF DVU2122 0.29 +2.2 2,217,859 + cpaF DVU2122 0.34 +1.0 2,217,882 + cpaF DVU2122 0.35 -1.6 2,217,900 - cpaF DVU2122 0.36 -1.4 2,217,922 - cpaF DVU2122 0.38 -0.8 2,217,960 + cpaF DVU2122 0.41 +4.8 2,217,963 - cpaF DVU2122 0.41 -1.5
Or see this region's nucleotide sequence