Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fabK and xerC are separated by 313 nucleotides xerC and DVU2067 overlap by 8 nucleotides
DVU2064: fabK - oxidoreductase, 2-nitropropane dioxygenase family (TIGR), at 2,144,757 to 2,145,878
fabK
DVU2066: xerC - site-specific recombinase, phage integrase family (TIGR), at 2,146,192 to 2,147,616
xerC
DVU2067: DVU2067 - GGDEF domain protein (TIGR), at 2,147,609 to 2,148,730
DVU2067
Position (kb)
2146
2147
2148 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 2145.960 kb on - strand at 2146.168 kb on - strand at 2146.310 kb on + strand at 2146.310 kb on + strand at 2146.352 kb on + strand, within xerC at 2146.414 kb on - strand, within xerC at 2146.455 kb on - strand, within xerC at 2146.457 kb on + strand, within xerC at 2146.457 kb on + strand, within xerC at 2146.511 kb on - strand, within xerC at 2146.512 kb on - strand, within xerC at 2146.536 kb on + strand, within xerC at 2146.544 kb on - strand, within xerC at 2146.605 kb on + strand, within xerC at 2146.605 kb on + strand, within xerC at 2146.607 kb on + strand, within xerC at 2146.692 kb on - strand, within xerC at 2146.718 kb on + strand, within xerC at 2146.805 kb on - strand, within xerC at 2147.065 kb on - strand, within xerC at 2147.131 kb on - strand, within xerC at 2147.373 kb on + strand, within xerC at 2147.530 kb on - strand at 2147.607 kb on + strand at 2147.709 kb on - strand at 2148.012 kb on - strand, within DVU2067 at 2148.125 kb on - strand, within DVU2067 at 2148.125 kb on - strand, within DVU2067 at 2148.125 kb on - strand, within DVU2067 at 2148.198 kb on - strand, within DVU2067 at 2148.198 kb on - strand, within DVU2067 at 2148.221 kb on + strand, within DVU2067 at 2148.603 kb on - strand, within DVU2067
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,145,960 - -1.7 2,146,168 - -1.7 2,146,310 + -0.2 2,146,310 + -2.6 2,146,352 + xerC DVU2066 0.11 +2.5 2,146,414 - xerC DVU2066 0.16 +2.2 2,146,455 - xerC DVU2066 0.18 -0.1 2,146,457 + xerC DVU2066 0.19 -2.3 2,146,457 + xerC DVU2066 0.19 -2.2 2,146,511 - xerC DVU2066 0.22 +2.5 2,146,512 - xerC DVU2066 0.22 +3.2 2,146,536 + xerC DVU2066 0.24 +2.8 2,146,544 - xerC DVU2066 0.25 +1.6 2,146,605 + xerC DVU2066 0.29 -1.3 2,146,605 + xerC DVU2066 0.29 +0.9 2,146,607 + xerC DVU2066 0.29 -0.8 2,146,692 - xerC DVU2066 0.35 +1.3 2,146,718 + xerC DVU2066 0.37 -2.0 2,146,805 - xerC DVU2066 0.43 +1.3 2,147,065 - xerC DVU2066 0.61 +1.8 2,147,131 - xerC DVU2066 0.66 +3.2 2,147,373 + xerC DVU2066 0.83 -0.6 2,147,530 - -0.5 2,147,607 + +1.1 2,147,709 - -0.7 2,148,012 - DVU2067 0.36 +0.6 2,148,125 - DVU2067 0.46 +2.2 2,148,125 - DVU2067 0.46 +1.0 2,148,125 - DVU2067 0.46 -1.1 2,148,198 - DVU2067 0.52 +0.4 2,148,198 - DVU2067 0.52 -0.1 2,148,221 + DVU2067 0.55 -1.0 2,148,603 - DVU2067 0.89 +1.8
Or see this region's nucleotide sequence