Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2032 and DVU2033 are separated by 71 nucleotides DVU2033 and DVU2034 are separated by 2 nucleotides DVU2034 and DVU2035 overlap by 4 nucleotides DVU2035 and DVU2036 overlap by 11 nucleotides DVU2036 and DVU2037 are separated by 78 nucleotides
DVU2032: DVU2032 - ERF family protein (TIGR), at 2,113,963 to 2,114,676
DVU2032
DVU2033: DVU2033 - hypothetical protein (TIGR), at 2,114,748 to 2,116,019
DVU2033
DVU2034: DVU2034 - hypothetical protein (TIGR), at 2,116,022 to 2,116,387
DVU2034
DVU2035: DVU2035 - plasmid stabilization system family protein (TIGR), at 2,116,384 to 2,116,671
DVU2035
DVU2036: DVU2036 - helix-turn-helix protein, CopG family (TIGR), at 2,116,661 to 2,116,900
DVU2036
DVU2037: DVU2037 - cobS protein, putative (TIGR), at 2,116,979 to 2,117,953
DVU2037
Position (kb)
2114
2115
2116
2117 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 2113.781 kb on + strand at 2113.781 kb on + strand at 2113.789 kb on - strand at 2113.789 kb on - strand at 2113.801 kb on + strand at 2113.970 kb on + strand at 2113.980 kb on - strand at 2113.980 kb on - strand at 2113.985 kb on + strand at 2113.985 kb on + strand at 2113.993 kb on - strand at 2113.993 kb on - strand at 2114.055 kb on - strand, within DVU2032 at 2114.130 kb on + strand, within DVU2032 at 2114.150 kb on + strand, within DVU2032 at 2114.216 kb on - strand, within DVU2032 at 2114.249 kb on - strand, within DVU2032 at 2114.258 kb on + strand, within DVU2032 at 2114.261 kb on - strand, within DVU2032 at 2114.261 kb on - strand, within DVU2032 at 2114.261 kb on - strand, within DVU2032 at 2114.261 kb on - strand, within DVU2032 at 2114.261 kb on - strand, within DVU2032 at 2114.261 kb on - strand, within DVU2032 at 2114.298 kb on - strand, within DVU2032 at 2114.340 kb on + strand, within DVU2032 at 2114.403 kb on + strand, within DVU2032 at 2114.472 kb on - strand, within DVU2032 at 2114.499 kb on - strand, within DVU2032 at 2114.536 kb on + strand, within DVU2032 at 2114.539 kb on - strand, within DVU2032 at 2114.661 kb on + strand at 2114.669 kb on - strand at 2114.708 kb on + strand at 2114.818 kb on - strand at 2114.869 kb on + strand at 2114.877 kb on - strand, within DVU2033 at 2114.925 kb on + strand, within DVU2033 at 2114.925 kb on + strand, within DVU2033 at 2114.988 kb on - strand, within DVU2033 at 2115.048 kb on - strand, within DVU2033 at 2115.053 kb on - strand, within DVU2033 at 2115.074 kb on - strand, within DVU2033 at 2115.149 kb on - strand, within DVU2033 at 2115.184 kb on - strand, within DVU2033 at 2115.188 kb on + strand, within DVU2033 at 2115.189 kb on - strand, within DVU2033 at 2115.197 kb on + strand, within DVU2033 at 2115.197 kb on + strand, within DVU2033 at 2115.216 kb on - strand, within DVU2033 at 2115.227 kb on + strand, within DVU2033 at 2115.490 kb on + strand, within DVU2033 at 2115.541 kb on - strand, within DVU2033 at 2115.591 kb on - strand, within DVU2033 at 2115.640 kb on - strand, within DVU2033 at 2115.640 kb on - strand, within DVU2033 at 2115.669 kb on - strand, within DVU2033 at 2115.752 kb on + strand, within DVU2033 at 2115.770 kb on - strand, within DVU2033 at 2115.793 kb on + strand, within DVU2033 at 2115.820 kb on + strand, within DVU2033 at 2115.820 kb on + strand, within DVU2033 at 2115.828 kb on - strand, within DVU2033 at 2115.828 kb on - strand, within DVU2033 at 2115.868 kb on + strand, within DVU2033 at 2115.876 kb on - strand, within DVU2033 at 2116.006 kb on + strand at 2116.020 kb on - strand at 2116.043 kb on + strand at 2116.157 kb on + strand, within DVU2034 at 2116.328 kb on + strand, within DVU2034 at 2116.345 kb on + strand, within DVU2034 at 2116.390 kb on + strand at 2116.611 kb on + strand, within DVU2035 at 2116.981 kb on + strand at 2116.981 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 2,113,781 + +1.6 2,113,781 + +0.1 2,113,789 - +0.8 2,113,789 - -2.9 2,113,801 + -1.8 2,113,970 + +1.1 2,113,980 - +0.9 2,113,980 - +1.0 2,113,985 + +2.8 2,113,985 + +0.9 2,113,993 - -0.4 2,113,993 - +1.6 2,114,055 - DVU2032 0.13 -1.4 2,114,130 + DVU2032 0.23 -1.8 2,114,150 + DVU2032 0.26 -0.9 2,114,216 - DVU2032 0.35 +2.2 2,114,249 - DVU2032 0.40 +1.6 2,114,258 + DVU2032 0.41 +2.2 2,114,261 - DVU2032 0.42 +1.5 2,114,261 - DVU2032 0.42 -0.4 2,114,261 - DVU2032 0.42 -2.3 2,114,261 - DVU2032 0.42 -1.5 2,114,261 - DVU2032 0.42 -2.3 2,114,261 - DVU2032 0.42 -2.5 2,114,298 - DVU2032 0.47 -1.1 2,114,340 + DVU2032 0.53 +0.7 2,114,403 + DVU2032 0.62 +1.1 2,114,472 - DVU2032 0.71 +1.2 2,114,499 - DVU2032 0.75 -2.1 2,114,536 + DVU2032 0.80 -1.5 2,114,539 - DVU2032 0.81 +2.5 2,114,661 + -2.4 2,114,669 - -2.0 2,114,708 + -0.1 2,114,818 - -1.7 2,114,869 + -2.1 2,114,877 - DVU2033 0.10 -0.8 2,114,925 + DVU2033 0.14 +0.6 2,114,925 + DVU2033 0.14 -1.7 2,114,988 - DVU2033 0.19 -2.4 2,115,048 - DVU2033 0.24 -1.5 2,115,053 - DVU2033 0.24 -0.1 2,115,074 - DVU2033 0.26 -2.0 2,115,149 - DVU2033 0.32 -1.3 2,115,184 - DVU2033 0.34 +3.2 2,115,188 + DVU2033 0.35 -0.0 2,115,189 - DVU2033 0.35 +0.1 2,115,197 + DVU2033 0.35 +1.6 2,115,197 + DVU2033 0.35 -0.9 2,115,216 - DVU2033 0.37 -1.3 2,115,227 + DVU2033 0.38 -1.9 2,115,490 + DVU2033 0.58 +3.2 2,115,541 - DVU2033 0.62 -1.8 2,115,591 - DVU2033 0.66 +1.0 2,115,640 - DVU2033 0.70 +0.1 2,115,640 - DVU2033 0.70 +2.4 2,115,669 - DVU2033 0.72 -0.7 2,115,752 + DVU2033 0.79 +0.7 2,115,770 - DVU2033 0.80 -1.6 2,115,793 + DVU2033 0.82 -1.2 2,115,820 + DVU2033 0.84 -1.8 2,115,820 + DVU2033 0.84 +0.2 2,115,828 - DVU2033 0.85 -2.1 2,115,828 - DVU2033 0.85 -0.7 2,115,868 + DVU2033 0.88 -2.7 2,115,876 - DVU2033 0.89 +0.2 2,116,006 + -1.6 2,116,020 - +1.8 2,116,043 + -2.3 2,116,157 + DVU2034 0.37 -1.2 2,116,328 + DVU2034 0.84 -1.7 2,116,345 + DVU2034 0.88 -1.8 2,116,390 + +1.1 2,116,611 + DVU2035 0.79 +2.0 2,116,981 + -2.0 2,116,981 + -1.3
Or see this region's nucleotide sequence