Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cysD and DVU1567 are separated by 9 nucleotides DVU1567 and ftn are separated by 163 nucleotides ftn and porA are separated by 72 nucleotides porA and porB overlap by 4 nucleotides
DVU1566: cysD - phosphoadenosine phosphosulfate reductase, putative (TIGR), at 1,649,064 to 1,649,738
cysD
DVU1567: DVU1567 - hypothetical protein (TIGR), at 1,649,748 to 1,649,960
DVU1567
DVU1568: ftn - ferritin (TIGR), at 1,650,124 to 1,650,633
ftn
DVU1569: porA - pyruvate ferredoxin oxidoreductase, alpha subunit (TIGR), at 1,650,706 to 1,652,442
porA
DVU1570: porB - pyruvate ferredoxin oxidoreductase, beta subunit (TIGR), at 1,652,439 to 1,653,293
porB
Position (kb)
1650
1651
1652
1653 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2
3
4 at 1649.745 kb on + strand at 1649.745 kb on + strand at 1649.747 kb on + strand at 1649.753 kb on - strand at 1649.769 kb on - strand at 1649.883 kb on + strand, within DVU1567 at 1649.978 kb on - strand at 1649.978 kb on - strand at 1649.994 kb on + strand at 1650.002 kb on - strand at 1650.026 kb on - strand at 1650.060 kb on + strand at 1650.060 kb on + strand at 1650.068 kb on - strand at 1650.170 kb on - strand at 1650.328 kb on + strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.383 kb on - strand, within ftn at 1650.386 kb on + strand, within ftn at 1650.408 kb on + strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.517 kb on - strand, within ftn at 1650.578 kb on - strand, within ftn at 1650.595 kb on - strand at 1650.685 kb on + strand at 1650.685 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.788 kb on + strand at 1650.796 kb on - strand at 1650.796 kb on - strand at 1650.833 kb on - strand at 1650.852 kb on + strand at 1650.858 kb on + strand at 1650.898 kb on + strand, within porA at 1650.926 kb on + strand, within porA at 1650.991 kb on - strand, within porA at 1651.011 kb on + strand, within porA at 1651.186 kb on - strand, within porA at 1651.194 kb on - strand, within porA at 1651.291 kb on - strand, within porA at 1651.292 kb on - strand, within porA at 1651.298 kb on - strand, within porA at 1651.392 kb on + strand, within porA at 1651.400 kb on - strand, within porA at 1651.400 kb on - strand, within porA at 1651.490 kb on - strand, within porA at 1651.621 kb on + strand, within porA at 1651.725 kb on - strand, within porA at 1651.737 kb on + strand, within porA at 1652.199 kb on + strand, within porA at 1652.207 kb on - strand, within porA at 1652.207 kb on - strand, within porA at 1652.208 kb on + strand, within porA at 1652.249 kb on + strand, within porA at 1652.253 kb on - strand, within porA at 1652.276 kb on + strand at 1652.483 kb on - strand at 1652.484 kb on - strand at 1652.665 kb on + strand, within porB at 1652.665 kb on + strand, within porB at 1652.671 kb on - strand, within porB at 1652.825 kb on + strand, within porB at 1653.107 kb on - strand, within porB at 1653.164 kb on - strand, within porB at 1653.208 kb on - strand at 1653.433 kb on - strand at 1653.433 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 1,649,745 + -3.0 1,649,745 + -1.5 1,649,747 + -1.1 1,649,753 - -2.6 1,649,769 - -1.5 1,649,883 + DVU1567 0.63 -1.3 1,649,978 - -2.3 1,649,978 - +2.5 1,649,994 + +0.4 1,650,002 - +0.1 1,650,026 - -1.3 1,650,060 + -2.4 1,650,060 + -0.5 1,650,068 - -1.2 1,650,170 - -1.9 1,650,328 + ftn DVU1568 0.40 -0.9 1,650,338 - ftn DVU1568 0.42 -1.0 1,650,338 - ftn DVU1568 0.42 -1.7 1,650,383 - ftn DVU1568 0.51 -2.0 1,650,386 + ftn DVU1568 0.51 -1.4 1,650,408 + ftn DVU1568 0.56 -1.3 1,650,512 - ftn DVU1568 0.76 -0.6 1,650,512 - ftn DVU1568 0.76 -1.1 1,650,517 - ftn DVU1568 0.77 -1.5 1,650,578 - ftn DVU1568 0.89 -1.8 1,650,595 - -0.9 1,650,685 + +0.7 1,650,685 + -2.2 1,650,788 + -2.1 1,650,788 + +1.5 1,650,788 + -1.9 1,650,788 + -0.7 1,650,796 - +2.0 1,650,796 - -1.2 1,650,833 - -0.6 1,650,852 + -0.2 1,650,858 + +1.5 1,650,898 + porA DVU1569 0.11 -3.8 1,650,926 + porA DVU1569 0.13 +2.5 1,650,991 - porA DVU1569 0.16 -2.6 1,651,011 + porA DVU1569 0.18 +1.4 1,651,186 - porA DVU1569 0.28 -2.4 1,651,194 - porA DVU1569 0.28 +1.5 1,651,291 - porA DVU1569 0.34 -2.1 1,651,292 - porA DVU1569 0.34 +3.8 1,651,298 - porA DVU1569 0.34 +0.4 1,651,392 + porA DVU1569 0.39 +0.5 1,651,400 - porA DVU1569 0.40 -2.0 1,651,400 - porA DVU1569 0.40 -1.7 1,651,490 - porA DVU1569 0.45 -2.1 1,651,621 + porA DVU1569 0.53 -1.9 1,651,725 - porA DVU1569 0.59 -2.2 1,651,737 + porA DVU1569 0.59 -2.0 1,652,199 + porA DVU1569 0.86 -0.7 1,652,207 - porA DVU1569 0.86 -1.2 1,652,207 - porA DVU1569 0.86 -2.0 1,652,208 + porA DVU1569 0.86 +0.4 1,652,249 + porA DVU1569 0.89 +2.5 1,652,253 - porA DVU1569 0.89 -0.6 1,652,276 + -1.8 1,652,483 - +2.2 1,652,484 - +2.0 1,652,665 + porB DVU1570 0.26 -1.0 1,652,665 + porB DVU1570 0.26 -1.8 1,652,671 - porB DVU1570 0.27 +1.4 1,652,825 + porB DVU1570 0.45 -2.7 1,653,107 - porB DVU1570 0.78 -0.3 1,653,164 - porB DVU1570 0.85 +0.5 1,653,208 - -2.2 1,653,433 - -2.6 1,653,433 - -1.4
Or see this region's nucleotide sequence