Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DORF35977 and tRNA-Arg are separated by 114 nucleotides tRNA-Arg and tRNA-Arg are separated by 19 nucleotides tRNA-Arg and tRNA-Arg are separated by 32 nucleotides tRNA-Arg and DORF11999 are separated by 47 nucleotides DORF11999 and cysD are separated by 76 nucleotides cysD and DVU1567 are separated by 9 nucleotides DVU1567 and ftn are separated by 163 nucleotides ftn and porA are separated by 72 nucleotides
DORF35977: DORF35977 - no description, at 1,647,759 to 1,648,106
DORF35977
DVU_tRNA-Arg-1: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,221 to 1,648,297
tRNA-Arg
DVU_tRNA-Arg-2: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,317 to 1,648,393
tRNA-Arg
DVU_tRNA-Arg-3: tRNA-Arg - tRNA with anticodon ACG for Arg (VIMSS-AUTO), at 1,648,426 to 1,648,502
tRNA-Arg
DORF11999: DORF11999 - SEED: FIG00606676: hypothetical protein, at 1,648,550 to 1,648,987
DORF11999
DVU1566: cysD - phosphoadenosine phosphosulfate reductase, putative (TIGR), at 1,649,064 to 1,649,738
cysD
DVU1567: DVU1567 - hypothetical protein (TIGR), at 1,649,748 to 1,649,960
DVU1567
DVU1568: ftn - ferritin (TIGR), at 1,650,124 to 1,650,633
ftn
DVU1569: porA - pyruvate ferredoxin oxidoreductase, alpha subunit (TIGR), at 1,650,706 to 1,652,442
porA
Position (kb) 1649
1650 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1648.258 kb on - strand, within tRNA-Arg at 1648.258 kb on - strand, within tRNA-Arg at 1648.758 kb on - strand, within DORF11999 at 1648.853 kb on - strand, within DORF11999 at 1648.853 kb on - strand, within DORF11999 at 1648.855 kb on + strand, within DORF11999 at 1648.871 kb on + strand, within DORF11999 at 1648.893 kb on + strand, within DORF11999 at 1649.111 kb on - strand at 1649.150 kb on + strand, within cysD at 1649.196 kb on - strand, within cysD at 1649.196 kb on - strand, within cysD at 1649.298 kb on + strand, within cysD at 1649.306 kb on - strand, within cysD at 1649.344 kb on + strand, within cysD at 1649.356 kb on - strand, within cysD at 1649.361 kb on - strand, within cysD at 1649.471 kb on + strand, within cysD at 1649.471 kb on + strand, within cysD at 1649.615 kb on + strand, within cysD at 1649.632 kb on + strand, within cysD at 1649.639 kb on + strand, within cysD at 1649.639 kb on + strand, within cysD at 1649.640 kb on + strand, within cysD at 1649.650 kb on + strand, within cysD at 1649.745 kb on + strand at 1649.745 kb on + strand at 1649.747 kb on + strand at 1649.753 kb on - strand at 1649.769 kb on - strand at 1649.883 kb on + strand, within DVU1567 at 1649.978 kb on - strand at 1649.978 kb on - strand at 1649.994 kb on + strand at 1650.002 kb on - strand at 1650.026 kb on - strand at 1650.060 kb on + strand at 1650.060 kb on + strand at 1650.068 kb on - strand at 1650.170 kb on - strand at 1650.328 kb on + strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.338 kb on - strand, within ftn at 1650.383 kb on - strand, within ftn at 1650.386 kb on + strand, within ftn at 1650.408 kb on + strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.512 kb on - strand, within ftn at 1650.517 kb on - strand, within ftn at 1650.578 kb on - strand, within ftn at 1650.595 kb on - strand at 1650.685 kb on + strand at 1650.685 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 +1.1 1,648,258 - tRNA-Arg DVU_tRNA-Arg-1 0.48 -0.8 1,648,758 - DORF11999 0.47 -1.9 1,648,853 - DORF11999 0.69 -1.2 1,648,853 - DORF11999 0.69 -1.3 1,648,855 + DORF11999 0.70 -2.5 1,648,871 + DORF11999 0.73 -1.1 1,648,893 + DORF11999 0.78 +1.8 1,649,111 - -0.4 1,649,150 + cysD DVU1566 0.13 +2.0 1,649,196 - cysD DVU1566 0.20 -2.8 1,649,196 - cysD DVU1566 0.20 -2.1 1,649,298 + cysD DVU1566 0.35 +1.6 1,649,306 - cysD DVU1566 0.36 -0.4 1,649,344 + cysD DVU1566 0.41 +0.9 1,649,356 - cysD DVU1566 0.43 -1.1 1,649,361 - cysD DVU1566 0.44 -1.6 1,649,471 + cysD DVU1566 0.60 -2.4 1,649,471 + cysD DVU1566 0.60 +0.1 1,649,615 + cysD DVU1566 0.82 -1.0 1,649,632 + cysD DVU1566 0.84 -1.3 1,649,639 + cysD DVU1566 0.85 +0.6 1,649,639 + cysD DVU1566 0.85 +0.6 1,649,640 + cysD DVU1566 0.85 -1.7 1,649,650 + cysD DVU1566 0.87 +1.4 1,649,745 + -3.0 1,649,745 + -1.5 1,649,747 + -1.1 1,649,753 - -2.6 1,649,769 - -1.5 1,649,883 + DVU1567 0.63 -1.3 1,649,978 - -2.3 1,649,978 - +2.5 1,649,994 + +0.4 1,650,002 - +0.1 1,650,026 - -1.3 1,650,060 + -2.4 1,650,060 + -0.5 1,650,068 - -1.2 1,650,170 - -1.9 1,650,328 + ftn DVU1568 0.40 -0.9 1,650,338 - ftn DVU1568 0.42 -1.0 1,650,338 - ftn DVU1568 0.42 -1.7 1,650,383 - ftn DVU1568 0.51 -2.0 1,650,386 + ftn DVU1568 0.51 -1.4 1,650,408 + ftn DVU1568 0.56 -1.3 1,650,512 - ftn DVU1568 0.76 -0.6 1,650,512 - ftn DVU1568 0.76 -1.1 1,650,517 - ftn DVU1568 0.77 -1.5 1,650,578 - ftn DVU1568 0.89 -1.8 1,650,595 - -0.9 1,650,685 + +0.7 1,650,685 + -2.2
Or see this region's nucleotide sequence