Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU1529 and DVU1530 are separated by 136 nucleotides DVU1530 and DVU1531 are separated by 125 nucleotides DVU1531 and coaD overlap by 25 nucleotides
DVU1529: DVU1529 - decarboxylase family protein (TIGR), at 1,597,222 to 1,597,878
DVU1529
DVU1530: DVU1530 - metallo-beta-lactamase family protein (TIGR), at 1,598,015 to 1,599,625
DVU1530
DVU1531: DVU1531 - methyltransferase, putative (TIGR), at 1,599,751 to 1,600,323
DVU1531
DVU1532: coaD - pantetheine-phosphate adenylyltransferase (TIGR), at 1,600,299 to 1,600,859
coaD
Position (kb)
1598
1599
1600 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1597.070 kb on - strand at 1597.151 kb on - strand at 1597.166 kb on + strand at 1597.166 kb on + strand at 1597.174 kb on - strand at 1597.174 kb on - strand at 1597.174 kb on - strand at 1597.186 kb on + strand at 1597.187 kb on + strand at 1597.276 kb on - strand at 1597.291 kb on + strand, within DVU1529 at 1597.425 kb on - strand, within DVU1529 at 1597.425 kb on - strand, within DVU1529 at 1597.468 kb on - strand, within DVU1529 at 1597.474 kb on + strand, within DVU1529 at 1597.535 kb on + strand, within DVU1529 at 1597.543 kb on + strand, within DVU1529 at 1597.548 kb on + strand, within DVU1529 at 1597.556 kb on - strand, within DVU1529 at 1597.721 kb on - strand, within DVU1529 at 1597.773 kb on - strand, within DVU1529 at 1597.807 kb on - strand, within DVU1529 at 1597.819 kb on - strand at 1597.823 kb on + strand at 1598.007 kb on - strand at 1598.074 kb on + strand at 1598.074 kb on + strand at 1598.082 kb on - strand at 1598.119 kb on - strand at 1598.209 kb on - strand, within DVU1530 at 1598.255 kb on + strand, within DVU1530 at 1598.272 kb on + strand, within DVU1530 at 1598.380 kb on + strand, within DVU1530 at 1598.550 kb on + strand, within DVU1530 at 1598.717 kb on + strand, within DVU1530 at 1598.785 kb on + strand, within DVU1530 at 1598.785 kb on + strand, within DVU1530 at 1598.793 kb on + strand, within DVU1530 at 1598.860 kb on + strand, within DVU1530 at 1598.860 kb on + strand, within DVU1530 at 1599.136 kb on - strand, within DVU1530 at 1599.205 kb on + strand, within DVU1530 at 1599.306 kb on - strand, within DVU1530 at 1599.390 kb on + strand, within DVU1530 at 1599.390 kb on + strand, within DVU1530 at 1599.496 kb on + strand at 1599.504 kb on - strand at 1599.544 kb on + strand at 1599.552 kb on - strand at 1600.007 kb on + strand, within DVU1531 at 1600.059 kb on + strand, within DVU1531 at 1600.177 kb on - strand, within DVU1531 at 1600.189 kb on - strand, within DVU1531 at 1600.206 kb on + strand, within DVU1531 at 1600.250 kb on + strand, within DVU1531 at 1600.258 kb on - strand, within DVU1531
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 1,597,070 - -0.0 1,597,151 - +1.0 1,597,166 + +1.1 1,597,166 + -1.8 1,597,174 - +3.2 1,597,174 - -0.2 1,597,174 - +3.8 1,597,186 + -0.0 1,597,187 + -1.9 1,597,276 - +2.2 1,597,291 + DVU1529 0.11 -0.5 1,597,425 - DVU1529 0.31 -1.2 1,597,425 - DVU1529 0.31 -2.4 1,597,468 - DVU1529 0.37 -2.2 1,597,474 + DVU1529 0.38 -2.8 1,597,535 + DVU1529 0.48 -1.8 1,597,543 + DVU1529 0.49 -2.4 1,597,548 + DVU1529 0.50 +0.6 1,597,556 - DVU1529 0.51 +2.5 1,597,721 - DVU1529 0.76 +2.5 1,597,773 - DVU1529 0.84 -2.7 1,597,807 - DVU1529 0.89 +0.1 1,597,819 - -0.3 1,597,823 + -3.2 1,598,007 - +0.4 1,598,074 + -0.1 1,598,074 + -0.9 1,598,082 - -0.4 1,598,119 - -0.2 1,598,209 - DVU1530 0.12 -1.0 1,598,255 + DVU1530 0.15 -2.1 1,598,272 + DVU1530 0.16 -1.5 1,598,380 + DVU1530 0.23 +0.4 1,598,550 + DVU1530 0.33 +1.6 1,598,717 + DVU1530 0.44 -0.3 1,598,785 + DVU1530 0.48 -1.0 1,598,785 + DVU1530 0.48 +0.8 1,598,793 + DVU1530 0.48 -0.7 1,598,860 + DVU1530 0.52 -0.8 1,598,860 + DVU1530 0.52 -1.5 1,599,136 - DVU1530 0.70 -1.3 1,599,205 + DVU1530 0.74 +1.5 1,599,306 - DVU1530 0.80 -0.2 1,599,390 + DVU1530 0.85 -2.7 1,599,390 + DVU1530 0.85 +1.4 1,599,496 + +1.8 1,599,504 - +1.7 1,599,544 + -1.2 1,599,552 - -2.3 1,600,007 + DVU1531 0.45 -0.5 1,600,059 + DVU1531 0.54 -0.9 1,600,177 - DVU1531 0.74 +0.0 1,600,189 - DVU1531 0.76 -0.4 1,600,206 + DVU1531 0.79 -0.3 1,600,250 + DVU1531 0.87 -1.7 1,600,258 - DVU1531 0.88 +0.1
Or see this region's nucleotide sequence