Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt fliM and truA are separated by 599 nucleotides truA and DVU0912 are separated by 1 nucleotides DVU0912 and DVU0913 are separated by 76 nucleotides
DVU0910: fliM - flagellar motor switch protein FliM (TIGR), at 1,004,227 to 1,005,207
fliM
DVU0911: truA - tRNA pseudouridine synthase A (TIGR), at 1,005,807 to 1,006,700
truA
DVU0912: DVU0912 - conserved domain protein (TIGR), at 1,006,702 to 1,007,286
DVU0912
DVU0913: DVU0913 - conserved hypothetical protein (TIGR), at 1,007,363 to 1,007,800
DVU0913
Position (kb)
1005
1006
1007 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3
4 at 1004.903 kb on + strand, within fliM at 1004.945 kb on - strand, within fliM at 1004.979 kb on + strand, within fliM at 1005.058 kb on - strand, within fliM at 1005.065 kb on - strand, within fliM at 1005.083 kb on - strand, within fliM at 1005.121 kb on - strand at 1005.229 kb on - strand at 1005.379 kb on + strand at 1005.482 kb on + strand at 1005.533 kb on - strand at 1005.577 kb on - strand at 1005.680 kb on + strand at 1005.723 kb on - strand at 1005.807 kb on - strand at 1005.963 kb on - strand, within truA at 1006.088 kb on - strand, within truA at 1006.112 kb on - strand, within truA at 1006.112 kb on - strand, within truA at 1006.130 kb on - strand, within truA at 1006.263 kb on + strand, within truA at 1006.270 kb on + strand, within truA at 1006.271 kb on - strand, within truA at 1006.294 kb on + strand, within truA at 1006.307 kb on - strand, within truA at 1006.313 kb on + strand, within truA at 1006.346 kb on + strand, within truA at 1006.482 kb on - strand, within truA at 1006.617 kb on - strand at 1006.643 kb on - strand at 1006.651 kb on - strand at 1006.689 kb on + strand at 1006.693 kb on - strand at 1006.841 kb on + strand, within DVU0912 at 1006.841 kb on - strand, within DVU0912 at 1006.921 kb on + strand, within DVU0912 at 1006.931 kb on + strand, within DVU0912 at 1007.188 kb on + strand, within DVU0912 at 1007.222 kb on + strand, within DVU0912 at 1007.222 kb on + strand, within DVU0912 at 1007.285 kb on + strand at 1007.295 kb on + strand at 1007.303 kb on - strand at 1007.547 kb on + strand, within DVU0913 at 1007.555 kb on - strand, within DVU0913 at 1007.576 kb on + strand, within DVU0913 at 1007.584 kb on - strand, within DVU0913 at 1007.584 kb on - strand, within DVU0913 at 1007.690 kb on - strand, within DVU0913
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 1,004,903 + fliM DVU0910 0.69 -2.2 1,004,945 - fliM DVU0910 0.73 -1.3 1,004,979 + fliM DVU0910 0.77 -0.4 1,005,058 - fliM DVU0910 0.85 +1.3 1,005,065 - fliM DVU0910 0.85 -1.6 1,005,083 - fliM DVU0910 0.87 +1.0 1,005,121 - +2.4 1,005,229 - +0.0 1,005,379 + -0.6 1,005,482 + +0.7 1,005,533 - -3.1 1,005,577 - -1.0 1,005,680 + -1.4 1,005,723 - -1.3 1,005,807 - -0.6 1,005,963 - truA DVU0911 0.17 +3.7 1,006,088 - truA DVU0911 0.31 -1.5 1,006,112 - truA DVU0911 0.34 -0.0 1,006,112 - truA DVU0911 0.34 -0.3 1,006,130 - truA DVU0911 0.36 -0.2 1,006,263 + truA DVU0911 0.51 +3.1 1,006,270 + truA DVU0911 0.52 +4.7 1,006,271 - truA DVU0911 0.52 +0.6 1,006,294 + truA DVU0911 0.54 -0.6 1,006,307 - truA DVU0911 0.56 -0.3 1,006,313 + truA DVU0911 0.57 -0.7 1,006,346 + truA DVU0911 0.60 +3.1 1,006,482 - truA DVU0911 0.76 -1.9 1,006,617 - -1.1 1,006,643 - +0.2 1,006,651 - +1.3 1,006,689 + -1.0 1,006,693 - -1.5 1,006,841 + DVU0912 0.24 +1.0 1,006,841 - DVU0912 0.24 +3.1 1,006,921 + DVU0912 0.37 -1.9 1,006,931 + DVU0912 0.39 +0.5 1,007,188 + DVU0912 0.83 +2.4 1,007,222 + DVU0912 0.89 -1.5 1,007,222 + DVU0912 0.89 +1.0 1,007,285 + -0.4 1,007,295 + -0.2 1,007,303 - +0.8 1,007,547 + DVU0913 0.42 +2.1 1,007,555 - DVU0913 0.44 -0.6 1,007,576 + DVU0913 0.49 +0.3 1,007,584 - DVU0913 0.50 +0.3 1,007,584 - DVU0913 0.50 +0.3 1,007,690 - DVU0913 0.75 -1.3
Or see this region's nucleotide sequence