Experiment: MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slt and DVUA0126 are separated by 373 nucleotides DVUA0126 and DORF2161 are separated by 59 nucleotides DORF2161 and cas3 are separated by 450 nucleotides
DVUA0125: slt - transglycosylase, SLT family (TIGR), at 163,070 to 164,422
slt
DVUA0126: DVUA0126 - conserved hypothetical protein (TIGR), at 164,796 to 165,263
DVUA0126
DORF2161: DORF2161 - SEED: FIG00606767: hypothetical protein, at 165,323 to 167,803
DORF2161
DVUA0129: cas3 - CRISPR-associated helicase Cas3 domain protein (TIGR), at 168,254 to 170,362
cas3
Position (kb)
165
166
167
168 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2
3 at 164.428 kb on + strand at 164.428 kb on + strand at 164.522 kb on + strand at 164.530 kb on - strand at 164.679 kb on - strand at 164.859 kb on + strand, within DVUA0126 at 164.919 kb on + strand, within DVUA0126 at 164.927 kb on - strand, within DVUA0126 at 165.000 kb on + strand, within DVUA0126 at 165.011 kb on + strand, within DVUA0126 at 165.127 kb on + strand, within DVUA0126 at 165.128 kb on + strand, within DVUA0126 at 165.139 kb on + strand, within DVUA0126 at 165.149 kb on - strand, within DVUA0126 at 165.184 kb on - strand, within DVUA0126 at 165.184 kb on - strand, within DVUA0126 at 165.204 kb on - strand, within DVUA0126 at 165.290 kb on + strand at 165.300 kb on - strand at 165.300 kb on - strand at 165.403 kb on - strand at 165.442 kb on + strand at 165.444 kb on + strand at 165.460 kb on - strand at 165.502 kb on + strand at 165.510 kb on - strand at 165.596 kb on + strand, within DORF2161 at 165.677 kb on - strand, within DORF2161 at 165.694 kb on + strand, within DORF2161 at 165.694 kb on + strand, within DORF2161 at 165.694 kb on + strand, within DORF2161 at 165.774 kb on - strand, within DORF2161 at 165.775 kb on - strand, within DORF2161 at 165.796 kb on + strand, within DORF2161 at 165.799 kb on - strand, within DORF2161 at 165.815 kb on + strand, within DORF2161 at 165.820 kb on + strand, within DORF2161 at 165.828 kb on - strand, within DORF2161 at 165.938 kb on - strand, within DORF2161 at 165.942 kb on + strand, within DORF2161 at 165.993 kb on - strand, within DORF2161 at 166.017 kb on - strand, within DORF2161 at 166.017 kb on - strand, within DORF2161 at 166.021 kb on + strand, within DORF2161 at 166.021 kb on - strand, within DORF2161 at 166.040 kb on - strand, within DORF2161 at 166.178 kb on - strand, within DORF2161 at 166.218 kb on - strand, within DORF2161 at 166.230 kb on + strand, within DORF2161 at 166.656 kb on - strand, within DORF2161 at 166.732 kb on + strand, within DORF2161 at 166.745 kb on + strand, within DORF2161 at 166.745 kb on + strand, within DORF2161 at 166.745 kb on + strand, within DORF2161 at 166.825 kb on + strand, within DORF2161 at 166.863 kb on - strand, within DORF2161 at 166.912 kb on - strand, within DORF2161 at 166.917 kb on - strand, within DORF2161 at 166.917 kb on - strand, within DORF2161 at 166.917 kb on - strand, within DORF2161 at 166.917 kb on - strand, within DORF2161 at 166.917 kb on - strand, within DORF2161 at 166.917 kb on - strand, within DORF2161 at 166.954 kb on - strand, within DORF2161 at 167.009 kb on - strand, within DORF2161 at 167.046 kb on + strand, within DORF2161 at 167.104 kb on - strand, within DORF2161 at 167.189 kb on - strand, within DORF2161 at 167.241 kb on + strand, within DORF2161 at 167.286 kb on + strand, within DORF2161 at 167.382 kb on - strand, within DORF2161 at 167.389 kb on + strand, within DORF2161 at 167.426 kb on + strand, within DORF2161 at 167.439 kb on - strand, within DORF2161 at 167.439 kb on - strand, within DORF2161 at 167.514 kb on + strand, within DORF2161 at 167.519 kb on + strand, within DORF2161 at 167.522 kb on - strand, within DORF2161 at 167.524 kb on + strand, within DORF2161 at 167.597 kb on + strand at 167.599 kb on - strand at 167.698 kb on - strand at 167.713 kb on + strand at 167.733 kb on - strand at 167.823 kb on + strand at 167.823 kb on + strand at 167.831 kb on - strand at 167.831 kb on - strand at 167.831 kb on - strand at 167.958 kb on - strand at 167.993 kb on + strand at 168.001 kb on - strand at 168.019 kb on + strand at 168.038 kb on + strand at 168.170 kb on - strand at 168.178 kb on - strand at 168.192 kb on - strand at 168.196 kb on + strand at 168.196 kb on + strand at 168.196 kb on + strand at 168.225 kb on + strand at 168.443 kb on - strand at 168.453 kb on - strand at 168.579 kb on - strand, within cas3 at 168.596 kb on + strand, within cas3 at 168.596 kb on + strand, within cas3 at 168.665 kb on - strand, within cas3 at 168.740 kb on + strand, within cas3 at 168.740 kb on + strand, within cas3 at 168.740 kb on + strand, within cas3 at 168.748 kb on - strand, within cas3 at 168.748 kb on - strand, within cas3 at 168.767 kb on + strand, within cas3 at 168.775 kb on - strand, within cas3 at 168.789 kb on - strand, within cas3
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin with Casaminos (vit. assay) 0.05% remove 164,428 + -1.6 164,428 + +1.9 164,522 + +0.1 164,530 - -1.9 164,679 - -1.4 164,859 + DVUA0126 0.13 +0.9 164,919 + DVUA0126 0.26 -1.0 164,927 - DVUA0126 0.28 -2.1 165,000 + DVUA0126 0.44 -0.1 165,011 + DVUA0126 0.46 -0.1 165,127 + DVUA0126 0.71 +1.2 165,128 + DVUA0126 0.71 -1.5 165,139 + DVUA0126 0.73 -2.9 165,149 - DVUA0126 0.75 -0.3 165,184 - DVUA0126 0.83 -1.6 165,184 - DVUA0126 0.83 +1.9 165,204 - DVUA0126 0.87 -0.3 165,290 + -1.6 165,300 - -1.1 165,300 - -0.3 165,403 - +2.1 165,442 + -1.7 165,444 + +1.3 165,460 - -1.7 165,502 + -1.0 165,510 - +0.7 165,596 + DORF2161 0.11 -2.8 165,677 - DORF2161 0.14 -1.4 165,694 + DORF2161 0.15 -0.0 165,694 + DORF2161 0.15 -2.3 165,694 + DORF2161 0.15 +1.0 165,774 - DORF2161 0.18 -1.2 165,775 - DORF2161 0.18 +0.3 165,796 + DORF2161 0.19 -1.5 165,799 - DORF2161 0.19 -0.0 165,815 + DORF2161 0.20 -0.2 165,820 + DORF2161 0.20 +2.7 165,828 - DORF2161 0.20 -1.4 165,938 - DORF2161 0.25 -1.2 165,942 + DORF2161 0.25 -1.0 165,993 - DORF2161 0.27 -2.0 166,017 - DORF2161 0.28 +0.1 166,017 - DORF2161 0.28 +1.3 166,021 + DORF2161 0.28 +0.7 166,021 - DORF2161 0.28 -1.9 166,040 - DORF2161 0.29 -0.7 166,178 - DORF2161 0.34 -2.3 166,218 - DORF2161 0.36 -1.9 166,230 + DORF2161 0.37 +0.0 166,656 - DORF2161 0.54 +2.7 166,732 + DORF2161 0.57 +0.3 166,745 + DORF2161 0.57 -0.9 166,745 + DORF2161 0.57 -1.0 166,745 + DORF2161 0.57 +1.2 166,825 + DORF2161 0.61 +2.7 166,863 - DORF2161 0.62 +1.5 166,912 - DORF2161 0.64 +1.0 166,917 - DORF2161 0.64 -0.1 166,917 - DORF2161 0.64 +2.9 166,917 - DORF2161 0.64 +1.1 166,917 - DORF2161 0.64 +0.2 166,917 - DORF2161 0.64 -0.2 166,917 - DORF2161 0.64 -1.6 166,954 - DORF2161 0.66 -1.0 167,009 - DORF2161 0.68 +1.1 167,046 + DORF2161 0.69 +0.6 167,104 - DORF2161 0.72 -2.0 167,189 - DORF2161 0.75 -1.9 167,241 + DORF2161 0.77 +1.7 167,286 + DORF2161 0.79 -1.1 167,382 - DORF2161 0.83 -2.4 167,389 + DORF2161 0.83 -1.9 167,426 + DORF2161 0.85 -0.4 167,439 - DORF2161 0.85 +3.7 167,439 - DORF2161 0.85 +0.1 167,514 + DORF2161 0.88 -1.3 167,519 + DORF2161 0.89 -0.8 167,522 - DORF2161 0.89 +2.3 167,524 + DORF2161 0.89 +1.3 167,597 + -2.0 167,599 - +0.7 167,698 - +1.3 167,713 + +1.9 167,733 - -2.0 167,823 + -1.1 167,823 + +2.1 167,831 - -1.5 167,831 - +0.9 167,831 - -1.9 167,958 - -2.0 167,993 + +1.5 168,001 - -1.2 168,019 + -1.2 168,038 + -1.4 168,170 - -0.1 168,178 - +1.1 168,192 - +1.0 168,196 + -1.8 168,196 + -1.3 168,196 + +2.1 168,225 + +0.5 168,443 - +0.4 168,453 - -1.2 168,579 - cas3 DVUA0129 0.15 +1.9 168,596 + cas3 DVUA0129 0.16 +0.6 168,596 + cas3 DVUA0129 0.16 -1.6 168,665 - cas3 DVUA0129 0.19 -0.5 168,740 + cas3 DVUA0129 0.23 -0.8 168,740 + cas3 DVUA0129 0.23 -1.8 168,740 + cas3 DVUA0129 0.23 -2.4 168,748 - cas3 DVUA0129 0.23 +0.6 168,748 - cas3 DVUA0129 0.23 -1.7 168,767 + cas3 DVUA0129 0.24 +1.7 168,775 - cas3 DVUA0129 0.25 +1.1 168,789 - cas3 DVUA0129 0.25 -0.6
Or see this region's nucleotide sequence