Experiment: MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt DVU2136 and sucCD are separated by 220 nucleotides sucCD and DVU2138 are separated by 36 nucleotides DVU2138 and aphA are separated by 52 nucleotides
DVU2136: DVU2136 - hypothetical protein (TIGR), at 2,231,969 to 2,233,150
DVU2136
DVU2137: sucCD - succinyl-CoA synthetase, alpha/beta subunit (sucCD) (Chris Hemme), at 2,233,371 to 2,235,500
sucCD
DVU2138: DVU2138 - conserved hypothetical protein (TIGR), at 2,235,537 to 2,236,190
DVU2138
DVU2139: aphA - histone deacetylase family protein (TIGR), at 2,236,243 to 2,237,562
aphA
Position (kb)
2233
2234
2235
2236 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2232.411 kb on + strand, within DVU2136 at 2232.411 kb on + strand, within DVU2136 at 2232.419 kb on - strand, within DVU2136 at 2232.446 kb on - strand, within DVU2136 at 2232.564 kb on + strand, within DVU2136 at 2232.572 kb on - strand, within DVU2136 at 2232.572 kb on - strand, within DVU2136 at 2232.572 kb on - strand, within DVU2136 at 2232.579 kb on + strand, within DVU2136 at 2232.587 kb on - strand, within DVU2136 at 2232.642 kb on + strand, within DVU2136 at 2232.650 kb on + strand, within DVU2136 at 2232.668 kb on - strand, within DVU2136 at 2232.730 kb on - strand, within DVU2136 at 2232.744 kb on - strand, within DVU2136 at 2232.746 kb on + strand, within DVU2136 at 2232.770 kb on + strand, within DVU2136 at 2232.841 kb on - strand, within DVU2136 at 2232.866 kb on + strand, within DVU2136 at 2232.881 kb on - strand, within DVU2136 at 2232.889 kb on + strand, within DVU2136 at 2232.895 kb on - strand, within DVU2136 at 2232.897 kb on - strand, within DVU2136 at 2232.929 kb on + strand, within DVU2136 at 2232.935 kb on + strand, within DVU2136 at 2233.035 kb on + strand at 2233.053 kb on - strand at 2233.092 kb on - strand at 2233.118 kb on + strand at 2233.222 kb on + strand at 2233.237 kb on - strand at 2233.327 kb on + strand at 2233.382 kb on - strand at 2233.502 kb on + strand at 2233.510 kb on - strand at 2233.663 kb on + strand, within sucCD at 2233.739 kb on - strand, within sucCD at 2233.935 kb on + strand, within sucCD at 2233.936 kb on - strand, within sucCD at 2233.954 kb on + strand, within sucCD at 2234.087 kb on - strand, within sucCD at 2234.087 kb on - strand, within sucCD at 2234.113 kb on + strand, within sucCD at 2234.121 kb on - strand, within sucCD at 2234.199 kb on + strand, within sucCD at 2234.214 kb on + strand, within sucCD at 2234.263 kb on - strand, within sucCD at 2234.675 kb on - strand, within sucCD at 2234.708 kb on + strand, within sucCD at 2235.017 kb on - strand, within sucCD at 2235.073 kb on - strand, within sucCD at 2235.143 kb on + strand, within sucCD at 2235.167 kb on - strand, within sucCD at 2235.236 kb on - strand, within sucCD at 2235.538 kb on + strand at 2235.702 kb on - strand, within DVU2138 at 2235.724 kb on - strand, within DVU2138 at 2235.754 kb on + strand, within DVU2138 at 2235.941 kb on + strand, within DVU2138 at 2235.993 kb on - strand, within DVU2138 at 2236.043 kb on + strand, within DVU2138 at 2236.170 kb on - strand at 2236.170 kb on - strand at 2236.171 kb on + strand at 2236.206 kb on + strand at 2236.214 kb on - strand at 2236.422 kb on - strand, within aphA at 2236.452 kb on + strand, within aphA at 2236.474 kb on - strand, within aphA
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05% remove 2,232,411 + DVU2136 0.37 -0.3 2,232,411 + DVU2136 0.37 +0.3 2,232,419 - DVU2136 0.38 +0.1 2,232,446 - DVU2136 0.40 -1.1 2,232,564 + DVU2136 0.50 -1.5 2,232,572 - DVU2136 0.51 +0.0 2,232,572 - DVU2136 0.51 -0.2 2,232,572 - DVU2136 0.51 -0.4 2,232,579 + DVU2136 0.52 -0.9 2,232,587 - DVU2136 0.52 -0.0 2,232,642 + DVU2136 0.57 +1.4 2,232,650 + DVU2136 0.58 +0.2 2,232,668 - DVU2136 0.59 -0.4 2,232,730 - DVU2136 0.64 -0.1 2,232,744 - DVU2136 0.66 -0.3 2,232,746 + DVU2136 0.66 -0.7 2,232,770 + DVU2136 0.68 -1.8 2,232,841 - DVU2136 0.74 -0.3 2,232,866 + DVU2136 0.76 -0.3 2,232,881 - DVU2136 0.77 -0.5 2,232,889 + DVU2136 0.78 -0.3 2,232,895 - DVU2136 0.78 +0.9 2,232,897 - DVU2136 0.79 -0.6 2,232,929 + DVU2136 0.81 +0.1 2,232,935 + DVU2136 0.82 -0.2 2,233,035 + -0.3 2,233,053 - -0.5 2,233,092 - -0.7 2,233,118 + -1.5 2,233,222 + +0.3 2,233,237 - -0.1 2,233,327 + -0.9 2,233,382 - -0.3 2,233,502 + -0.0 2,233,510 - -0.3 2,233,663 + sucCD DVU2137 0.14 +1.9 2,233,739 - sucCD DVU2137 0.17 +0.7 2,233,935 + sucCD DVU2137 0.26 +0.4 2,233,936 - sucCD DVU2137 0.27 +1.2 2,233,954 + sucCD DVU2137 0.27 +1.8 2,234,087 - sucCD DVU2137 0.34 -0.9 2,234,087 - sucCD DVU2137 0.34 -1.8 2,234,113 + sucCD DVU2137 0.35 -0.6 2,234,121 - sucCD DVU2137 0.35 +1.4 2,234,199 + sucCD DVU2137 0.39 -0.4 2,234,214 + sucCD DVU2137 0.40 -1.1 2,234,263 - sucCD DVU2137 0.42 -0.5 2,234,675 - sucCD DVU2137 0.61 -0.2 2,234,708 + sucCD DVU2137 0.63 -0.6 2,235,017 - sucCD DVU2137 0.77 -0.2 2,235,073 - sucCD DVU2137 0.80 -0.1 2,235,143 + sucCD DVU2137 0.83 -0.1 2,235,167 - sucCD DVU2137 0.84 -1.0 2,235,236 - sucCD DVU2137 0.88 -0.6 2,235,538 + +2.4 2,235,702 - DVU2138 0.25 -0.4 2,235,724 - DVU2138 0.29 -0.7 2,235,754 + DVU2138 0.33 +0.5 2,235,941 + DVU2138 0.62 -0.5 2,235,993 - DVU2138 0.70 +2.2 2,236,043 + DVU2138 0.77 +0.5 2,236,170 - -0.2 2,236,170 - -0.1 2,236,171 + -0.7 2,236,206 + -0.2 2,236,214 - +0.6 2,236,422 - aphA DVU2139 0.14 -0.0 2,236,452 + aphA DVU2139 0.16 -0.0 2,236,474 - aphA DVU2139 0.17 +0.6
Or see this region's nucleotide sequence