Experiment: MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ppiB-1 and hisH are separated by 187 nucleotides hisH and hisF overlap by 11 nucleotides hisF and DORF26924 are separated by 82 nucleotides
DVU0284: ppiB-1 - peptidyl-prolyl cis-trans isomerase B (TIGR), at 330,771 to 331,277
ppiB-1
DVU0285: hisH - IGP synthase, amidotransferase subunit (EC 4.3.2.10) <small>(from data)</small>, at 331,465 to 332,106
hisH
DVU0286: hisF - imidazoleglycerol phosphate synthase, cyclase subunit (TIGR), at 332,096 to 332,875
hisF
DORF26924: DORF26924 - no description, at 332,958 to 333,728
DORF26924
Position (kb) 332
333 Strain fitness (log2 ratio) -1
0
1
2
3 at 331.109 kb on - strand, within ppiB-1 at 331.110 kb on - strand, within ppiB-1 at 331.125 kb on + strand, within ppiB-1 at 331.131 kb on + strand, within ppiB-1 at 331.133 kb on - strand, within ppiB-1 at 331.133 kb on - strand, within ppiB-1 at 331.196 kb on + strand, within ppiB-1 at 331.229 kb on - strand at 331.239 kb on - strand at 331.257 kb on + strand at 331.277 kb on + strand at 331.277 kb on + strand at 331.284 kb on + strand at 331.284 kb on + strand at 331.353 kb on + strand at 331.417 kb on + strand at 331.495 kb on + strand at 331.592 kb on + strand, within hisH at 331.640 kb on - strand, within hisH at 331.655 kb on - strand, within hisH at 331.724 kb on + strand, within hisH at 331.852 kb on + strand, within hisH at 331.852 kb on + strand, within hisH at 331.852 kb on + strand, within hisH at 331.910 kb on - strand, within hisH at 331.910 kb on - strand, within hisH at 331.933 kb on - strand, within hisH at 331.980 kb on - strand, within hisH at 331.985 kb on - strand, within hisH at 331.995 kb on - strand, within hisH at 331.997 kb on + strand, within hisH at 332.004 kb on + strand, within hisH at 332.019 kb on + strand, within hisH at 332.056 kb on - strand at 332.121 kb on + strand at 332.129 kb on - strand at 332.129 kb on - strand at 332.179 kb on - strand, within hisF at 332.219 kb on - strand, within hisF at 332.271 kb on + strand, within hisF at 332.387 kb on + strand, within hisF at 332.458 kb on - strand, within hisF at 332.495 kb on + strand, within hisF at 332.554 kb on + strand, within hisF at 332.609 kb on + strand, within hisF at 332.615 kb on - strand, within hisF at 332.740 kb on + strand, within hisF at 332.755 kb on + strand, within hisF at 333.775 kb on + strand at 333.862 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05% remove 331,109 - ppiB-1 DVU0284 0.67 +0.2 331,110 - ppiB-1 DVU0284 0.67 -0.1 331,125 + ppiB-1 DVU0284 0.70 -0.7 331,131 + ppiB-1 DVU0284 0.71 -0.5 331,133 - ppiB-1 DVU0284 0.71 -0.5 331,133 - ppiB-1 DVU0284 0.71 -0.7 331,196 + ppiB-1 DVU0284 0.84 +0.0 331,229 - +0.6 331,239 - +0.2 331,257 + +2.0 331,277 + +0.3 331,277 + -0.0 331,284 + -0.4 331,284 + -0.1 331,353 + -1.1 331,417 + -0.4 331,495 + +2.8 331,592 + hisH DVU0285 0.20 -0.1 331,640 - hisH DVU0285 0.27 -0.9 331,655 - hisH DVU0285 0.30 -0.8 331,724 + hisH DVU0285 0.40 -1.1 331,852 + hisH DVU0285 0.60 +0.8 331,852 + hisH DVU0285 0.60 +0.1 331,852 + hisH DVU0285 0.60 -0.9 331,910 - hisH DVU0285 0.69 +1.2 331,910 - hisH DVU0285 0.69 +0.6 331,933 - hisH DVU0285 0.73 +2.0 331,980 - hisH DVU0285 0.80 -0.0 331,985 - hisH DVU0285 0.81 -0.5 331,995 - hisH DVU0285 0.83 -0.9 331,997 + hisH DVU0285 0.83 -1.1 332,004 + hisH DVU0285 0.84 -0.1 332,019 + hisH DVU0285 0.86 -0.5 332,056 - -0.4 332,121 + -1.5 332,129 - +0.6 332,129 - -0.5 332,179 - hisF DVU0286 0.11 -0.6 332,219 - hisF DVU0286 0.16 -0.9 332,271 + hisF DVU0286 0.22 -0.9 332,387 + hisF DVU0286 0.37 -0.4 332,458 - hisF DVU0286 0.46 -1.4 332,495 + hisF DVU0286 0.51 -1.2 332,554 + hisF DVU0286 0.59 -1.1 332,609 + hisF DVU0286 0.66 -0.4 332,615 - hisF DVU0286 0.67 +0.6 332,740 + hisF DVU0286 0.83 +0.1 332,755 + hisF DVU0286 0.84 +0.4 333,775 + +0.2 333,862 + -0.1
Or see this region's nucleotide sequence