Experiment: MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisI and hisG overlap by 4 nucleotides hisG and aroE are separated by 99 nucleotides
DVU0113: hisI - phosphoribosyl-AMP cyclohydrolase (TIGR), at 147,117 to 147,497
hisI
DVU0114: hisG - ATP phosphoribosyltransferase (EC 2.4.2.17) (from data) , at 147,494 to 148,375
hisG
DVU0115: aroE - shikimate 5-dehydrogenase (TIGR), at 148,475 to 149,380
aroE
Position (kb)
147
148
149 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 146.506 kb on + strand at 146.542 kb on - strand at 146.547 kb on - strand at 146.563 kb on - strand at 146.575 kb on - strand at 146.612 kb on + strand at 146.627 kb on + strand at 146.627 kb on + strand at 146.652 kb on + strand at 146.660 kb on - strand at 146.704 kb on - strand at 146.704 kb on - strand at 146.708 kb on + strand at 146.739 kb on - strand at 146.796 kb on - strand at 146.881 kb on - strand at 146.882 kb on + strand at 146.883 kb on + strand at 146.912 kb on + strand at 146.912 kb on + strand at 146.912 kb on + strand at 146.920 kb on - strand at 146.939 kb on + strand at 146.939 kb on + strand at 146.947 kb on - strand at 146.948 kb on + strand at 146.956 kb on - strand at 146.971 kb on + strand at 146.971 kb on + strand at 147.098 kb on - strand at 147.215 kb on - strand, within hisI at 147.298 kb on - strand, within hisI at 147.304 kb on + strand, within hisI at 147.468 kb on - strand at 147.483 kb on - strand at 147.517 kb on - strand at 147.527 kb on + strand at 147.535 kb on - strand at 147.560 kb on - strand at 147.649 kb on - strand, within hisG at 147.680 kb on - strand, within hisG at 147.760 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.821 kb on + strand, within hisG at 147.828 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.829 kb on - strand, within hisG at 147.877 kb on - strand, within hisG at 147.894 kb on - strand, within hisG at 147.897 kb on - strand, within hisG at 147.946 kb on + strand, within hisG at 148.020 kb on - strand, within hisG at 148.071 kb on + strand, within hisG at 148.124 kb on - strand, within hisG at 148.172 kb on - strand, within hisG at 148.221 kb on + strand, within hisG at 148.280 kb on + strand, within hisG at 148.311 kb on + strand at 148.327 kb on + strand at 148.341 kb on - strand at 148.355 kb on + strand at 148.355 kb on + strand at 148.385 kb on + strand at 148.471 kb on + strand at 148.495 kb on - strand at 148.577 kb on + strand, within aroE at 149.321 kb on + strand at 149.321 kb on + strand at 149.321 kb on + strand at 149.322 kb on + strand at 149.324 kb on - strand at 149.329 kb on - strand at 149.374 kb on - strand at 149.375 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05% remove 146,506 + +0.2 146,542 - +0.4 146,547 - +1.0 146,563 - +1.4 146,575 - -0.3 146,612 + -0.2 146,627 + -0.0 146,627 + -0.4 146,652 + -0.4 146,660 - +1.6 146,704 - -0.2 146,704 - -0.4 146,708 + -0.8 146,739 - -0.8 146,796 - +0.0 146,881 - -0.5 146,882 + -0.0 146,883 + +0.8 146,912 + -0.0 146,912 + -0.0 146,912 + -0.1 146,920 - -0.0 146,939 + +0.2 146,939 + +1.2 146,947 - -2.3 146,948 + -0.4 146,956 - +0.2 146,971 + -0.6 146,971 + +0.6 147,098 - -0.2 147,215 - hisI DVU0113 0.26 -0.3 147,298 - hisI DVU0113 0.48 -1.0 147,304 + hisI DVU0113 0.49 +1.2 147,468 - -0.1 147,483 - -0.8 147,517 - +1.9 147,527 + -2.3 147,535 - +2.2 147,560 - -1.0 147,649 - hisG DVU0114 0.18 -1.1 147,680 - hisG DVU0114 0.21 -1.4 147,760 + hisG DVU0114 0.30 +1.6 147,821 + hisG DVU0114 0.37 +2.2 147,821 + hisG DVU0114 0.37 -0.9 147,828 - hisG DVU0114 0.38 -1.3 147,829 - hisG DVU0114 0.38 -1.9 147,829 - hisG DVU0114 0.38 +1.2 147,877 - hisG DVU0114 0.43 +0.6 147,894 - hisG DVU0114 0.45 -0.5 147,897 - hisG DVU0114 0.46 +0.2 147,946 + hisG DVU0114 0.51 -1.6 148,020 - hisG DVU0114 0.60 -0.4 148,071 + hisG DVU0114 0.65 +1.3 148,124 - hisG DVU0114 0.71 +0.6 148,172 - hisG DVU0114 0.77 +0.6 148,221 + hisG DVU0114 0.82 -0.1 148,280 + hisG DVU0114 0.89 +1.6 148,311 + +0.8 148,327 + +1.6 148,341 - -0.6 148,355 + -1.0 148,355 + +0.2 148,385 + +0.3 148,471 + -0.8 148,495 - -0.9 148,577 + aroE DVU0115 0.11 -1.1 149,321 + -2.9 149,321 + -0.9 149,321 + -3.8 149,322 + -1.6 149,324 - -2.8 149,329 - -1.0 149,374 - -0.6 149,375 - -0.1
Or see this region's nucleotide sequence