Experiment: MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiE-1 and moeB are separated by 268 nucleotides moeB and thiH overlap by 16 nucleotides thiH and thiG are separated by 2 nucleotides thiG and thiS overlap by 1 nucleotides
DVU2091: thiE-1 - thiamine-phosphate pyrophosphorylase (TIGR), at 2,180,539 to 2,181,219
thiE-1
DVU2092: moeB - thiF family protein (TIGR), at 2,181,488 to 2,182,129
moeB
DVU2093: thiH - thiH protein (TIGR), at 2,182,114 to 2,183,448
thiH
DVU2094: thiG - thiG protein (TIGR), at 2,183,451 to 2,184,248
thiG
DVU2095: thiS - thiamine biosynthesis protein ThiS (TIGR), at 2,184,248 to 2,184,448
thiS
Position (kb)
2182
2183
2184 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 2181.192 kb on + strand at 2181.236 kb on - strand at 2181.406 kb on + strand at 2181.432 kb on - strand at 2181.583 kb on + strand, within moeB at 2181.647 kb on - strand, within moeB at 2181.762 kb on + strand, within moeB at 2181.762 kb on + strand, within moeB at 2181.875 kb on - strand, within moeB at 2181.934 kb on - strand, within moeB at 2182.010 kb on - strand, within moeB at 2182.083 kb on - strand at 2182.139 kb on - strand at 2182.204 kb on - strand at 2182.219 kb on - strand at 2182.258 kb on - strand, within thiH at 2182.258 kb on - strand, within thiH at 2182.278 kb on + strand, within thiH at 2182.393 kb on + strand, within thiH at 2182.401 kb on - strand, within thiH at 2182.474 kb on + strand, within thiH at 2182.853 kb on + strand, within thiH at 2182.878 kb on - strand, within thiH at 2182.887 kb on + strand, within thiH at 2183.038 kb on - strand, within thiH at 2183.038 kb on - strand, within thiH at 2183.055 kb on + strand, within thiH at 2183.063 kb on + strand, within thiH at 2183.120 kb on - strand, within thiH at 2183.125 kb on - strand, within thiH at 2183.159 kb on - strand, within thiH at 2183.193 kb on - strand, within thiH at 2183.195 kb on + strand, within thiH at 2183.220 kb on - strand, within thiH at 2183.222 kb on + strand, within thiH at 2183.229 kb on + strand, within thiH at 2183.229 kb on + strand, within thiH at 2183.237 kb on - strand, within thiH at 2183.284 kb on + strand, within thiH at 2183.344 kb on - strand at 2183.430 kb on + strand at 2183.502 kb on + strand at 2183.524 kb on - strand at 2183.525 kb on - strand at 2183.563 kb on + strand, within thiG at 2183.569 kb on - strand, within thiG at 2183.613 kb on + strand, within thiG at 2183.647 kb on + strand, within thiG at 2183.746 kb on + strand, within thiG at 2183.836 kb on + strand, within thiG at 2183.952 kb on + strand, within thiG at 2183.983 kb on + strand, within thiG at 2184.212 kb on + strand at 2184.252 kb on - strand at 2184.282 kb on + strand, within thiS at 2184.290 kb on - strand, within thiS at 2184.290 kb on - strand, within thiS at 2184.336 kb on + strand, within thiS at 2184.380 kb on + strand, within thiS at 2184.397 kb on + strand, within thiS at 2184.405 kb on - strand, within thiS
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05% remove 2,181,192 + -0.8 2,181,236 - -0.3 2,181,406 + -1.1 2,181,432 - -1.1 2,181,583 + moeB DVU2092 0.15 -0.3 2,181,647 - moeB DVU2092 0.25 +0.8 2,181,762 + moeB DVU2092 0.43 -1.2 2,181,762 + moeB DVU2092 0.43 -1.3 2,181,875 - moeB DVU2092 0.60 -2.4 2,181,934 - moeB DVU2092 0.69 -1.8 2,182,010 - moeB DVU2092 0.81 -1.5 2,182,083 - -1.3 2,182,139 - -0.4 2,182,204 - +0.1 2,182,219 - -0.1 2,182,258 - thiH DVU2093 0.11 -3.2 2,182,258 - thiH DVU2093 0.11 -1.5 2,182,278 + thiH DVU2093 0.12 -2.0 2,182,393 + thiH DVU2093 0.21 -2.1 2,182,401 - thiH DVU2093 0.21 -0.7 2,182,474 + thiH DVU2093 0.27 -2.1 2,182,853 + thiH DVU2093 0.55 -0.7 2,182,878 - thiH DVU2093 0.57 -1.6 2,182,887 + thiH DVU2093 0.58 -2.6 2,183,038 - thiH DVU2093 0.69 +0.3 2,183,038 - thiH DVU2093 0.69 -1.1 2,183,055 + thiH DVU2093 0.70 -2.0 2,183,063 + thiH DVU2093 0.71 -1.5 2,183,120 - thiH DVU2093 0.75 -1.6 2,183,125 - thiH DVU2093 0.76 +0.0 2,183,159 - thiH DVU2093 0.78 -2.5 2,183,193 - thiH DVU2093 0.81 -1.5 2,183,195 + thiH DVU2093 0.81 -1.4 2,183,220 - thiH DVU2093 0.83 -1.3 2,183,222 + thiH DVU2093 0.83 -1.5 2,183,229 + thiH DVU2093 0.84 -1.9 2,183,229 + thiH DVU2093 0.84 -3.2 2,183,237 - thiH DVU2093 0.84 -1.4 2,183,284 + thiH DVU2093 0.88 -1.4 2,183,344 - -1.2 2,183,430 + -1.0 2,183,502 + -1.2 2,183,524 - -0.9 2,183,525 - -1.4 2,183,563 + thiG DVU2094 0.14 -1.3 2,183,569 - thiG DVU2094 0.15 -2.0 2,183,613 + thiG DVU2094 0.20 -2.9 2,183,647 + thiG DVU2094 0.25 -1.8 2,183,746 + thiG DVU2094 0.37 -0.5 2,183,836 + thiG DVU2094 0.48 -3.1 2,183,952 + thiG DVU2094 0.63 -1.8 2,183,983 + thiG DVU2094 0.67 -1.5 2,184,212 + -0.5 2,184,252 - -1.2 2,184,282 + thiS DVU2095 0.17 -1.1 2,184,290 - thiS DVU2095 0.21 -1.4 2,184,290 - thiS DVU2095 0.21 -0.5 2,184,336 + thiS DVU2095 0.44 -1.6 2,184,380 + thiS DVU2095 0.66 -1.7 2,184,397 + thiS DVU2095 0.74 -1.6 2,184,405 - thiS DVU2095 0.78 -1.1
Or see this region's nucleotide sequence