Experiment: MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt thiH and aspA overlap by 14 nucleotides aspA and DVU1767 overlap by 4 nucleotides DVU1767 and DVU1768 are separated by 4 nucleotides
DVU1765: thiH - thiH protein, putative (TIGR), at 1,827,335 to 1,828,729
thiH
DVU1766: aspA - aspartate ammonia-lyase, putative (TIGR), at 1,828,716 to 1,830,128
aspA
DVU1767: DVU1767 - radical SAM domain protein (TIGR), at 1,830,125 to 1,831,093
DVU1767
DVU1768: DVU1768 - GTP-binding protein (TIGR), at 1,831,098 to 1,832,330
DVU1768
Position (kb)
1828
1829
1830
1831 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1827.769 kb on - strand, within thiH at 1827.876 kb on + strand, within thiH at 1827.958 kb on - strand, within thiH at 1827.992 kb on + strand, within thiH at 1828.000 kb on - strand, within thiH at 1828.100 kb on - strand, within thiH at 1828.101 kb on + strand, within thiH at 1828.109 kb on - strand, within thiH at 1828.119 kb on - strand, within thiH at 1828.127 kb on - strand, within thiH at 1828.241 kb on - strand, within thiH at 1828.342 kb on - strand, within thiH at 1828.468 kb on - strand, within thiH at 1828.499 kb on + strand, within thiH at 1828.506 kb on + strand, within thiH at 1828.507 kb on - strand, within thiH at 1828.615 kb on - strand at 1828.728 kb on + strand at 1828.788 kb on + strand at 1828.788 kb on + strand at 1828.796 kb on - strand at 1828.796 kb on - strand at 1828.796 kb on - strand at 1828.796 kb on - strand at 1828.820 kb on + strand at 1828.820 kb on + strand at 1828.820 kb on + strand at 1828.828 kb on - strand at 1828.828 kb on - strand at 1829.010 kb on + strand, within aspA at 1829.010 kb on + strand, within aspA at 1829.162 kb on + strand, within aspA at 1829.170 kb on - strand, within aspA at 1829.172 kb on - strand, within aspA at 1829.463 kb on + strand, within aspA at 1829.463 kb on + strand, within aspA at 1829.463 kb on + strand, within aspA at 1829.471 kb on - strand, within aspA at 1829.471 kb on - strand, within aspA at 1829.498 kb on - strand, within aspA at 1829.636 kb on - strand, within aspA at 1829.712 kb on - strand, within aspA at 1829.738 kb on + strand, within aspA at 1829.772 kb on - strand, within aspA at 1829.897 kb on + strand, within aspA at 1830.108 kb on + strand at 1830.113 kb on + strand at 1830.137 kb on - strand at 1830.160 kb on + strand at 1830.168 kb on - strand at 1830.176 kb on + strand at 1830.179 kb on - strand at 1830.179 kb on - strand at 1830.196 kb on + strand at 1830.204 kb on - strand at 1830.204 kb on - strand at 1830.204 kb on - strand at 1830.214 kb on - strand at 1830.224 kb on - strand, within DVU1767 at 1830.325 kb on + strand, within DVU1767 at 1830.338 kb on - strand, within DVU1767 at 1830.428 kb on + strand, within DVU1767 at 1830.516 kb on + strand, within DVU1767 at 1830.521 kb on + strand, within DVU1767 at 1830.529 kb on - strand, within DVU1767 at 1830.543 kb on - strand, within DVU1767 at 1830.646 kb on + strand, within DVU1767 at 1830.809 kb on + strand, within DVU1767 at 1830.861 kb on - strand, within DVU1767 at 1831.127 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05% remove 1,827,769 - thiH DVU1765 0.31 +0.3 1,827,876 + thiH DVU1765 0.39 -1.8 1,827,958 - thiH DVU1765 0.45 +0.6 1,827,992 + thiH DVU1765 0.47 -0.2 1,828,000 - thiH DVU1765 0.48 -0.4 1,828,100 - thiH DVU1765 0.55 -0.5 1,828,101 + thiH DVU1765 0.55 +0.8 1,828,109 - thiH DVU1765 0.55 +0.6 1,828,119 - thiH DVU1765 0.56 +1.0 1,828,127 - thiH DVU1765 0.57 -0.9 1,828,241 - thiH DVU1765 0.65 +0.0 1,828,342 - thiH DVU1765 0.72 -0.3 1,828,468 - thiH DVU1765 0.81 -0.1 1,828,499 + thiH DVU1765 0.83 -1.5 1,828,506 + thiH DVU1765 0.84 -2.0 1,828,507 - thiH DVU1765 0.84 +0.4 1,828,615 - -0.0 1,828,728 + -0.2 1,828,788 + +0.7 1,828,788 + +0.0 1,828,796 - +0.7 1,828,796 - +0.5 1,828,796 - +0.1 1,828,796 - -1.0 1,828,820 + +0.4 1,828,820 + -0.6 1,828,820 + +0.6 1,828,828 - +0.1 1,828,828 - +0.8 1,829,010 + aspA DVU1766 0.21 +2.4 1,829,010 + aspA DVU1766 0.21 -0.4 1,829,162 + aspA DVU1766 0.32 -1.0 1,829,170 - aspA DVU1766 0.32 -1.1 1,829,172 - aspA DVU1766 0.32 -0.8 1,829,463 + aspA DVU1766 0.53 -1.7 1,829,463 + aspA DVU1766 0.53 -0.5 1,829,463 + aspA DVU1766 0.53 -0.2 1,829,471 - aspA DVU1766 0.53 -0.1 1,829,471 - aspA DVU1766 0.53 -0.8 1,829,498 - aspA DVU1766 0.55 -2.6 1,829,636 - aspA DVU1766 0.65 -1.0 1,829,712 - aspA DVU1766 0.70 -0.1 1,829,738 + aspA DVU1766 0.72 -0.9 1,829,772 - aspA DVU1766 0.75 -0.1 1,829,897 + aspA DVU1766 0.84 -0.1 1,830,108 + +0.4 1,830,113 + -0.0 1,830,137 - -0.1 1,830,160 + -0.3 1,830,168 - -3.0 1,830,176 + -0.5 1,830,179 - +0.8 1,830,179 - +0.3 1,830,196 + +0.7 1,830,204 - -0.1 1,830,204 - -0.1 1,830,204 - +0.8 1,830,214 - -0.5 1,830,224 - DVU1767 0.10 -0.5 1,830,325 + DVU1767 0.21 -1.3 1,830,338 - DVU1767 0.22 -0.5 1,830,428 + DVU1767 0.31 -1.1 1,830,516 + DVU1767 0.40 -1.9 1,830,521 + DVU1767 0.41 -0.6 1,830,529 - DVU1767 0.42 -1.0 1,830,543 - DVU1767 0.43 +0.0 1,830,646 + DVU1767 0.54 -0.3 1,830,809 + DVU1767 0.71 -0.5 1,830,861 - DVU1767 0.76 -0.4 1,831,127 + -0.8
Or see this region's nucleotide sequence