Experiment: MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05%
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ptsI and DVU0982 are separated by 37 nucleotides DVU0982 and DVU0983 are separated by 1 nucleotides DVU0983 and miaB overlap by 7 nucleotides
DVU0981: ptsI - multiphosphoryl transfer protein, putative (TIGR), at 1,076,460 to 1,079,024
ptsI
DVU0982: DVU0982 - PHP domain protein (TIGR), at 1,079,062 to 1,079,721
DVU0982
DVU0983: DVU0983 - conserved hypothetical protein (TIGR), at 1,079,723 to 1,080,268
DVU0983
DVU0984: miaB - tRNA-i(6)A37 modification enzyme MiaB (TIGR), at 1,080,262 to 1,081,611
miaB
Position (kb)
1079
1080
1081 Strain fitness (log2 ratio)
-1
0
1 at 1078.834 kb on - strand at 1078.840 kb on - strand at 1078.974 kb on + strand at 1079.042 kb on - strand at 1079.102 kb on - strand at 1079.124 kb on + strand at 1079.134 kb on + strand, within DVU0982 at 1079.282 kb on - strand, within DVU0982 at 1079.339 kb on - strand, within DVU0982 at 1079.374 kb on + strand, within DVU0982 at 1079.422 kb on + strand, within DVU0982 at 1079.431 kb on - strand, within DVU0982 at 1079.555 kb on - strand, within DVU0982 at 1079.656 kb on + strand at 1079.808 kb on + strand, within DVU0983 at 1079.813 kb on + strand, within DVU0983 at 1079.821 kb on - strand, within DVU0983 at 1079.935 kb on + strand, within DVU0983 at 1080.086 kb on - strand, within DVU0983 at 1080.272 kb on + strand at 1080.285 kb on - strand at 1080.310 kb on - strand at 1080.334 kb on - strand at 1080.345 kb on + strand at 1080.345 kb on + strand at 1080.353 kb on - strand at 1080.381 kb on - strand at 1080.502 kb on + strand, within miaB at 1080.512 kb on + strand, within miaB at 1080.512 kb on + strand, within miaB at 1080.521 kb on + strand, within miaB at 1080.536 kb on + strand, within miaB at 1080.582 kb on + strand, within miaB at 1080.590 kb on - strand, within miaB at 1080.673 kb on - strand, within miaB at 1080.721 kb on - strand, within miaB at 1080.799 kb on + strand, within miaB at 1080.868 kb on - strand, within miaB at 1080.892 kb on - strand, within miaB at 1080.924 kb on - strand, within miaB at 1081.012 kb on + strand, within miaB at 1081.047 kb on - strand, within miaB at 1081.047 kb on - strand, within miaB at 1081.047 kb on - strand, within miaB at 1081.086 kb on + strand, within miaB at 1081.094 kb on - strand, within miaB at 1081.124 kb on + strand, within miaB at 1081.151 kb on + strand, within miaB at 1081.194 kb on - strand, within miaB
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without Pantothenic with Casamino acids (vit. assay) 0.05% remove 1,078,834 - +0.3 1,078,840 - -1.3 1,078,974 + +0.1 1,079,042 - +0.0 1,079,102 - +0.1 1,079,124 + -0.3 1,079,134 + DVU0982 0.11 -1.1 1,079,282 - DVU0982 0.33 +0.8 1,079,339 - DVU0982 0.42 -0.2 1,079,374 + DVU0982 0.47 -0.6 1,079,422 + DVU0982 0.55 -0.6 1,079,431 - DVU0982 0.56 +0.3 1,079,555 - DVU0982 0.75 +0.2 1,079,656 + -0.0 1,079,808 + DVU0983 0.16 -1.7 1,079,813 + DVU0983 0.16 -0.3 1,079,821 - DVU0983 0.18 -0.4 1,079,935 + DVU0983 0.39 -1.1 1,080,086 - DVU0983 0.66 -1.0 1,080,272 + -1.2 1,080,285 - -0.4 1,080,310 - +0.2 1,080,334 - -0.1 1,080,345 + -1.4 1,080,345 + +0.6 1,080,353 - -1.4 1,080,381 - -0.1 1,080,502 + miaB DVU0984 0.18 -0.3 1,080,512 + miaB DVU0984 0.19 +0.1 1,080,512 + miaB DVU0984 0.19 -0.3 1,080,521 + miaB DVU0984 0.19 -0.3 1,080,536 + miaB DVU0984 0.20 +0.1 1,080,582 + miaB DVU0984 0.24 -0.5 1,080,590 - miaB DVU0984 0.24 +0.3 1,080,673 - miaB DVU0984 0.30 -1.0 1,080,721 - miaB DVU0984 0.34 -0.4 1,080,799 + miaB DVU0984 0.40 -0.2 1,080,868 - miaB DVU0984 0.45 +0.1 1,080,892 - miaB DVU0984 0.47 -0.5 1,080,924 - miaB DVU0984 0.49 -0.1 1,081,012 + miaB DVU0984 0.56 -0.3 1,081,047 - miaB DVU0984 0.58 +0.3 1,081,047 - miaB DVU0984 0.58 +0.2 1,081,047 - miaB DVU0984 0.58 -0.5 1,081,086 + miaB DVU0984 0.61 -0.2 1,081,094 - miaB DVU0984 0.62 -0.5 1,081,124 + miaB DVU0984 0.64 -0.3 1,081,151 + miaB DVU0984 0.66 +0.8 1,081,194 - miaB DVU0984 0.69 -0.2
Or see this region's nucleotide sequence