Experiment: No vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_887 and Pf6N2E2_888 are separated by 365 nucleotides
Pf6N2E2_887 - hypothetical protein, at 1,040,365 to 1,042,548
_887
Pf6N2E2_888 - Enoyl-CoA hydratase (EC 4.2.1.17), at 1,042,914 to 1,043,459
_888
Position (kb)
1040
1041
1042
1043 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1039.366 kb on + strand at 1039.366 kb on + strand at 1039.367 kb on - strand at 1039.552 kb on - strand at 1039.552 kb on - strand at 1039.556 kb on - strand at 1039.628 kb on + strand at 1039.628 kb on + strand at 1039.659 kb on + strand at 1039.659 kb on + strand at 1039.660 kb on - strand at 1039.705 kb on + strand at 1040.511 kb on + strand at 1040.576 kb on + strand at 1040.577 kb on - strand at 1040.751 kb on - strand, within Pf6N2E2_887 at 1040.875 kb on + strand, within Pf6N2E2_887 at 1040.875 kb on + strand, within Pf6N2E2_887 at 1040.875 kb on + strand, within Pf6N2E2_887 at 1040.875 kb on + strand, within Pf6N2E2_887 at 1040.876 kb on - strand, within Pf6N2E2_887 at 1040.877 kb on + strand, within Pf6N2E2_887 at 1040.957 kb on - strand, within Pf6N2E2_887 at 1040.991 kb on + strand, within Pf6N2E2_887 at 1040.991 kb on + strand, within Pf6N2E2_887 at 1040.991 kb on + strand, within Pf6N2E2_887 at 1040.991 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.995 kb on + strand, within Pf6N2E2_887 at 1040.996 kb on - strand, within Pf6N2E2_887 at 1040.996 kb on - strand, within Pf6N2E2_887 at 1040.996 kb on - strand, within Pf6N2E2_887 at 1041.016 kb on + strand, within Pf6N2E2_887 at 1041.016 kb on + strand, within Pf6N2E2_887 at 1041.016 kb on + strand, within Pf6N2E2_887 at 1041.117 kb on - strand, within Pf6N2E2_887 at 1041.137 kb on + strand, within Pf6N2E2_887 at 1041.276 kb on + strand, within Pf6N2E2_887 at 1041.276 kb on + strand, within Pf6N2E2_887 at 1041.286 kb on + strand, within Pf6N2E2_887 at 1041.378 kb on + strand, within Pf6N2E2_887 at 1041.392 kb on + strand, within Pf6N2E2_887 at 1041.392 kb on + strand, within Pf6N2E2_887 at 1041.392 kb on + strand, within Pf6N2E2_887 at 1041.392 kb on + strand, within Pf6N2E2_887 at 1041.392 kb on + strand, within Pf6N2E2_887 at 1041.393 kb on - strand, within Pf6N2E2_887 at 1041.393 kb on - strand, within Pf6N2E2_887 at 1041.425 kb on + strand, within Pf6N2E2_887 at 1041.467 kb on + strand, within Pf6N2E2_887 at 1041.467 kb on + strand, within Pf6N2E2_887 at 1041.467 kb on + strand, within Pf6N2E2_887 at 1041.468 kb on - strand, within Pf6N2E2_887 at 1041.486 kb on + strand, within Pf6N2E2_887 at 1041.487 kb on - strand, within Pf6N2E2_887 at 1041.549 kb on + strand, within Pf6N2E2_887 at 1041.596 kb on + strand, within Pf6N2E2_887 at 1041.615 kb on + strand, within Pf6N2E2_887 at 1041.618 kb on + strand, within Pf6N2E2_887 at 1041.715 kb on + strand, within Pf6N2E2_887 at 1041.815 kb on + strand, within Pf6N2E2_887 at 1041.884 kb on + strand, within Pf6N2E2_887 at 1041.884 kb on + strand, within Pf6N2E2_887 at 1041.888 kb on + strand, within Pf6N2E2_887 at 1041.889 kb on - strand, within Pf6N2E2_887 at 1041.890 kb on + strand, within Pf6N2E2_887 at 1041.938 kb on - strand, within Pf6N2E2_887 at 1042.112 kb on + strand, within Pf6N2E2_887 at 1042.112 kb on + strand, within Pf6N2E2_887 at 1042.113 kb on - strand, within Pf6N2E2_887 at 1042.113 kb on - strand, within Pf6N2E2_887 at 1042.160 kb on - strand, within Pf6N2E2_887 at 1042.371 kb on - strand at 1042.416 kb on + strand at 1042.416 kb on + strand at 1042.416 kb on + strand at 1042.417 kb on - strand at 1042.417 kb on - strand at 1042.417 kb on - strand at 1042.417 kb on - strand at 1042.447 kb on + strand at 1042.447 kb on + strand at 1042.447 kb on + strand at 1042.448 kb on - strand at 1042.448 kb on - strand at 1042.448 kb on - strand at 1042.450 kb on + strand at 1042.538 kb on + strand at 1042.539 kb on - strand at 1042.546 kb on + strand at 1042.547 kb on - strand at 1042.673 kb on + strand at 1042.733 kb on + strand at 1042.733 kb on + strand at 1042.734 kb on - strand at 1042.734 kb on - strand at 1043.279 kb on - strand, within Pf6N2E2_888 at 1043.301 kb on + strand, within Pf6N2E2_888 at 1043.302 kb on - strand, within Pf6N2E2_888 at 1043.355 kb on + strand, within Pf6N2E2_888 at 1043.355 kb on + strand, within Pf6N2E2_888 at 1043.355 kb on + strand, within Pf6N2E2_888 at 1043.407 kb on - strand at 1043.462 kb on - strand at 1043.462 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t2 remove 1,039,366 + -0.7 1,039,366 + +0.2 1,039,367 - +1.2 1,039,552 - -2.0 1,039,552 - -1.1 1,039,556 - +0.6 1,039,628 + -0.4 1,039,628 + -0.1 1,039,659 + -0.4 1,039,659 + +1.2 1,039,660 - +1.6 1,039,705 + -1.4 1,040,511 + +0.3 1,040,576 + -1.4 1,040,577 - -0.4 1,040,751 - Pf6N2E2_887 0.18 -1.4 1,040,875 + Pf6N2E2_887 0.23 -0.4 1,040,875 + Pf6N2E2_887 0.23 -0.4 1,040,875 + Pf6N2E2_887 0.23 -1.4 1,040,875 + Pf6N2E2_887 0.23 -0.1 1,040,876 - Pf6N2E2_887 0.23 +0.2 1,040,877 + Pf6N2E2_887 0.23 -3.2 1,040,957 - Pf6N2E2_887 0.27 -0.4 1,040,991 + Pf6N2E2_887 0.29 +0.6 1,040,991 + Pf6N2E2_887 0.29 -0.4 1,040,991 + Pf6N2E2_887 0.29 -1.4 1,040,991 + Pf6N2E2_887 0.29 -2.7 1,040,995 + Pf6N2E2_887 0.29 -1.4 1,040,995 + Pf6N2E2_887 0.29 -0.4 1,040,995 + Pf6N2E2_887 0.29 +1.2 1,040,995 + Pf6N2E2_887 0.29 -0.1 1,040,995 + Pf6N2E2_887 0.29 -1.7 1,040,995 + Pf6N2E2_887 0.29 +0.2 1,040,995 + Pf6N2E2_887 0.29 -1.1 1,040,995 + Pf6N2E2_887 0.29 -2.0 1,040,996 - Pf6N2E2_887 0.29 -0.4 1,040,996 - Pf6N2E2_887 0.29 +0.9 1,040,996 - Pf6N2E2_887 0.29 -0.4 1,041,016 + Pf6N2E2_887 0.30 -1.0 1,041,016 + Pf6N2E2_887 0.30 -1.7 1,041,016 + Pf6N2E2_887 0.30 +0.8 1,041,117 - Pf6N2E2_887 0.34 -0.6 1,041,137 + Pf6N2E2_887 0.35 +1.2 1,041,276 + Pf6N2E2_887 0.42 -0.4 1,041,276 + Pf6N2E2_887 0.42 -0.4 1,041,286 + Pf6N2E2_887 0.42 -0.4 1,041,378 + Pf6N2E2_887 0.46 -0.8 1,041,392 + Pf6N2E2_887 0.47 -1.4 1,041,392 + Pf6N2E2_887 0.47 -0.4 1,041,392 + Pf6N2E2_887 0.47 -1.7 1,041,392 + Pf6N2E2_887 0.47 +0.6 1,041,392 + Pf6N2E2_887 0.47 +1.2 1,041,393 - Pf6N2E2_887 0.47 -0.4 1,041,393 - Pf6N2E2_887 0.47 +0.2 1,041,425 + Pf6N2E2_887 0.49 -0.4 1,041,467 + Pf6N2E2_887 0.50 +0.2 1,041,467 + Pf6N2E2_887 0.50 -0.4 1,041,467 + Pf6N2E2_887 0.50 -1.4 1,041,468 - Pf6N2E2_887 0.51 -0.4 1,041,486 + Pf6N2E2_887 0.51 -0.4 1,041,487 - Pf6N2E2_887 0.51 +1.2 1,041,549 + Pf6N2E2_887 0.54 -0.4 1,041,596 + Pf6N2E2_887 0.56 -1.2 1,041,615 + Pf6N2E2_887 0.57 -1.4 1,041,618 + Pf6N2E2_887 0.57 -2.4 1,041,715 + Pf6N2E2_887 0.62 +1.2 1,041,815 + Pf6N2E2_887 0.66 -0.4 1,041,884 + Pf6N2E2_887 0.70 -3.2 1,041,884 + Pf6N2E2_887 0.70 -0.4 1,041,888 + Pf6N2E2_887 0.70 -0.4 1,041,889 - Pf6N2E2_887 0.70 +0.6 1,041,890 + Pf6N2E2_887 0.70 +0.6 1,041,938 - Pf6N2E2_887 0.72 -3.2 1,042,112 + Pf6N2E2_887 0.80 -2.0 1,042,112 + Pf6N2E2_887 0.80 -2.4 1,042,113 - Pf6N2E2_887 0.80 -0.4 1,042,113 - Pf6N2E2_887 0.80 -1.4 1,042,160 - Pf6N2E2_887 0.82 -0.4 1,042,371 - -0.7 1,042,416 + -0.4 1,042,416 + -0.4 1,042,416 + -1.4 1,042,417 - -0.4 1,042,417 - +0.0 1,042,417 - +0.2 1,042,417 - -0.1 1,042,447 + -1.0 1,042,447 + -0.4 1,042,447 + -0.9 1,042,448 - -0.2 1,042,448 - -0.4 1,042,448 - -0.4 1,042,450 + +0.6 1,042,538 + -0.4 1,042,539 - -1.7 1,042,546 + +1.2 1,042,547 - -0.4 1,042,673 + -0.1 1,042,733 + -1.0 1,042,733 + +0.0 1,042,734 - -3.0 1,042,734 - -0.4 1,043,279 - Pf6N2E2_888 0.67 -1.4 1,043,301 + Pf6N2E2_888 0.71 -0.4 1,043,302 - Pf6N2E2_888 0.71 +0.6 1,043,355 + Pf6N2E2_888 0.81 +0.2 1,043,355 + Pf6N2E2_888 0.81 -0.4 1,043,355 + Pf6N2E2_888 0.81 -0.8 1,043,407 - +0.2 1,043,462 - -0.4 1,043,462 - +0.3
Or see this region's nucleotide sequence