Experiment: No vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_58 and Pf6N2E2_59 are separated by 120 nucleotides Pf6N2E2_59 and Pf6N2E2_60 are separated by 193 nucleotides Pf6N2E2_60 and Pf6N2E2_61 are separated by 69 nucleotides Pf6N2E2_61 and Pf6N2E2_62 overlap by 31 nucleotides
Pf6N2E2_58 - tRNA-dihydrouridine synthase C (EC 1.-.-.-), at 96,886 to 97,845
_58
Pf6N2E2_59 - 16 kDa heat shock protein A, at 97,966 to 98,418
_59
Pf6N2E2_60 - Sensory box/GGDEF family protein, at 98,612 to 101,890
_60
Pf6N2E2_61 - Cys regulon transcriptional activator CysB, at 101,960 to 102,238
_61
Pf6N2E2_62 - Cys regulon transcriptional activator CysB, at 102,208 to 102,852
_62
Position (kb) 98
99
100
101
102 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.889 kb on + strand at 97.894 kb on - strand at 97.894 kb on - strand at 97.971 kb on + strand at 98.072 kb on - strand, within Pf6N2E2_59 at 98.072 kb on - strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.080 kb on + strand, within Pf6N2E2_59 at 98.081 kb on - strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.227 kb on + strand, within Pf6N2E2_59 at 98.228 kb on - strand, within Pf6N2E2_59 at 98.462 kb on + strand at 98.468 kb on + strand at 98.468 kb on + strand at 98.469 kb on - strand at 98.469 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.531 kb on - strand at 98.546 kb on + strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.547 kb on - strand at 98.614 kb on - strand at 98.664 kb on + strand at 98.664 kb on + strand at 98.665 kb on - strand at 98.673 kb on + strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.674 kb on - strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.675 kb on + strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 98.676 kb on - strand at 99.034 kb on + strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.035 kb on - strand, within Pf6N2E2_60 at 99.450 kb on + strand, within Pf6N2E2_60 at 99.516 kb on - strand, within Pf6N2E2_60 at 100.141 kb on - strand, within Pf6N2E2_60 at 100.414 kb on + strand, within Pf6N2E2_60 at 100.414 kb on + strand, within Pf6N2E2_60 at 100.414 kb on + strand, within Pf6N2E2_60 at 100.503 kb on - strand, within Pf6N2E2_60 at 100.776 kb on + strand, within Pf6N2E2_60 at 100.809 kb on + strand, within Pf6N2E2_60 at 100.809 kb on + strand, within Pf6N2E2_60 at 100.821 kb on + strand, within Pf6N2E2_60 at 100.821 kb on + strand, within Pf6N2E2_60 at 100.822 kb on - strand, within Pf6N2E2_60 at 100.822 kb on - strand, within Pf6N2E2_60 at 101.101 kb on + strand, within Pf6N2E2_60 at 101.101 kb on + strand, within Pf6N2E2_60 at 101.241 kb on - strand, within Pf6N2E2_60 at 101.331 kb on + strand, within Pf6N2E2_60 at 101.649 kb on - strand at 101.661 kb on + strand at 101.662 kb on - strand at 101.662 kb on - strand at 101.891 kb on + strand at 101.921 kb on + strand at 101.922 kb on - strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.961 kb on + strand at 101.962 kb on - strand at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.052 kb on + strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.053 kb on - strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.054 kb on + strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.055 kb on - strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.059 kb on + strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.060 kb on - strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.077 kb on + strand, within Pf6N2E2_61 at 102.078 kb on - strand, within Pf6N2E2_61 at 102.168 kb on - strand, within Pf6N2E2_61 at 102.180 kb on + strand, within Pf6N2E2_61 at 102.250 kb on + strand at 102.393 kb on - strand at 102.455 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.550 kb on + strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.551 kb on - strand, within Pf6N2E2_62 at 102.823 kb on + strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.824 kb on - strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.825 kb on + strand at 102.826 kb on - strand at 102.826 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t1 remove 97,889 + -0.5 97,889 + +0.6 97,889 + +0.8 97,889 + +0.2 97,889 + -0.6 97,889 + -0.6 97,889 + -0.2 97,894 - -1.2 97,894 - -0.2 97,971 + -0.9 98,072 - Pf6N2E2_59 0.23 +1.4 98,072 - Pf6N2E2_59 0.23 -0.6 98,080 + Pf6N2E2_59 0.25 -0.2 98,080 + Pf6N2E2_59 0.25 +0.5 98,080 + Pf6N2E2_59 0.25 -0.5 98,080 + Pf6N2E2_59 0.25 +1.0 98,081 - Pf6N2E2_59 0.25 +1.2 98,227 + Pf6N2E2_59 0.58 -0.2 98,227 + Pf6N2E2_59 0.58 -0.6 98,228 - Pf6N2E2_59 0.58 +0.6 98,462 + -0.2 98,468 + -0.9 98,468 + -0.2 98,469 - -0.2 98,469 - -0.9 98,531 - -0.5 98,531 - +0.4 98,531 - +1.3 98,531 - -0.8 98,546 + +1.1 98,547 - -0.9 98,547 - -0.7 98,547 - -0.2 98,614 - -0.2 98,664 + +0.3 98,664 + -1.6 98,665 - +0.5 98,673 + +0.8 98,674 - -0.2 98,674 - -1.2 98,674 - +0.5 98,675 + +1.4 98,675 + -0.2 98,675 + -0.6 98,675 + -0.5 98,675 + +0.8 98,675 + +0.3 98,675 + -1.2 98,675 + -0.9 98,675 + +0.3 98,675 + -0.6 98,675 + +0.2 98,676 - -0.9 98,676 - -0.4 98,676 - +0.1 98,676 - +0.1 98,676 - -0.6 98,676 - -0.8 98,676 - -0.2 98,676 - -2.5 98,676 - +1.8 98,676 - +0.8 98,676 - -1.8 98,676 - -0.6 98,676 - -1.5 98,676 - +0.4 99,034 + Pf6N2E2_60 0.13 -0.2 99,035 - Pf6N2E2_60 0.13 -0.3 99,035 - Pf6N2E2_60 0.13 -1.8 99,035 - Pf6N2E2_60 0.13 -1.5 99,450 + Pf6N2E2_60 0.26 -0.2 99,516 - Pf6N2E2_60 0.28 +1.8 100,141 - Pf6N2E2_60 0.47 -2.8 100,414 + Pf6N2E2_60 0.55 +0.4 100,414 + Pf6N2E2_60 0.55 -1.8 100,414 + Pf6N2E2_60 0.55 +0.0 100,503 - Pf6N2E2_60 0.58 -0.2 100,776 + Pf6N2E2_60 0.66 -0.7 100,809 + Pf6N2E2_60 0.67 -0.2 100,809 + Pf6N2E2_60 0.67 +0.4 100,821 + Pf6N2E2_60 0.67 -0.2 100,821 + Pf6N2E2_60 0.67 -3.1 100,822 - Pf6N2E2_60 0.67 -0.5 100,822 - Pf6N2E2_60 0.67 -1.1 101,101 + Pf6N2E2_60 0.76 -0.2 101,101 + Pf6N2E2_60 0.76 -1.2 101,241 - Pf6N2E2_60 0.80 -0.2 101,331 + Pf6N2E2_60 0.83 -0.7 101,649 - +0.3 101,661 + -0.2 101,662 - -0.5 101,662 - -0.3 101,891 + -0.1 101,921 + -0.4 101,922 - -1.1 101,961 + +1.3 101,961 + -0.4 101,961 + -0.2 101,961 + -0.4 101,961 + +0.8 101,962 - -3.0 102,052 + Pf6N2E2_61 0.33 -0.5 102,052 + Pf6N2E2_61 0.33 -0.1 102,052 + Pf6N2E2_61 0.33 -0.2 102,052 + Pf6N2E2_61 0.33 +0.4 102,052 + Pf6N2E2_61 0.33 -1.6 102,052 + Pf6N2E2_61 0.33 -0.2 102,052 + Pf6N2E2_61 0.33 +2.1 102,052 + Pf6N2E2_61 0.33 +0.1 102,053 - Pf6N2E2_61 0.33 -0.2 102,053 - Pf6N2E2_61 0.33 +0.2 102,053 - Pf6N2E2_61 0.33 -0.2 102,053 - Pf6N2E2_61 0.33 +0.6 102,053 - Pf6N2E2_61 0.33 +0.8 102,054 + Pf6N2E2_61 0.34 +1.4 102,054 + Pf6N2E2_61 0.34 -1.2 102,054 + Pf6N2E2_61 0.34 +1.3 102,055 - Pf6N2E2_61 0.34 -1.2 102,055 - Pf6N2E2_61 0.34 -1.2 102,055 - Pf6N2E2_61 0.34 -1.2 102,055 - Pf6N2E2_61 0.34 -1.8 102,059 + Pf6N2E2_61 0.35 -0.5 102,059 + Pf6N2E2_61 0.35 -0.2 102,060 - Pf6N2E2_61 0.36 +0.4 102,060 - Pf6N2E2_61 0.36 -0.8 102,077 + Pf6N2E2_61 0.42 +0.8 102,077 + Pf6N2E2_61 0.42 -1.6 102,077 + Pf6N2E2_61 0.42 -0.2 102,077 + Pf6N2E2_61 0.42 -1.2 102,078 - Pf6N2E2_61 0.42 -1.8 102,168 - Pf6N2E2_61 0.75 -1.6 102,180 + Pf6N2E2_61 0.79 +0.8 102,250 + -0.2 102,393 - +1.4 102,455 + Pf6N2E2_62 0.38 -0.4 102,550 + Pf6N2E2_62 0.53 +0.5 102,550 + Pf6N2E2_62 0.53 +0.1 102,551 - Pf6N2E2_62 0.53 -1.8 102,551 - Pf6N2E2_62 0.53 +1.2 102,551 - Pf6N2E2_62 0.53 +2.0 102,551 - Pf6N2E2_62 0.53 -0.4 102,823 + -0.2 102,824 - -1.8 102,824 - -1.5 102,824 - -1.2 102,824 - -0.4 102,825 + -0.2 102,825 + +0.5 102,825 + -1.8 102,825 + +0.8 102,826 - -1.8 102,826 - -0.2
Or see this region's nucleotide sequence