Experiment: No vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2363 and Pf6N2E2_2364 are separated by 4 nucleotides Pf6N2E2_2364 and Pf6N2E2_2365 are separated by 8 nucleotides Pf6N2E2_2365 and Pf6N2E2_2366 overlap by 8 nucleotides Pf6N2E2_2366 and Pf6N2E2_2367 are separated by 541 nucleotides
Pf6N2E2_2363 - Type III secretion inner membrane protein (YscT,HrcT,SpaR,EscT,EpaR1,homologous to flagellar export components), at 2,798,350 to 2,799,153
_2363
Pf6N2E2_2364 - Type III secretion inner membrane protein (YscS,homologous to flagellar export components), at 2,799,158 to 2,799,412
_2364
Pf6N2E2_2365 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 2,799,421 to 2,800,080
_2365
Pf6N2E2_2366 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components), at 2,800,073 to 2,800,453
_2366
Pf6N2E2_2367 - hypothetical protein, at 2,800,995 to 2,801,870
_2367
Position (kb)
2800
2801 Strain fitness (log2 ratio)
-2
-1
0
1 at 2799.125 kb on + strand at 2799.126 kb on - strand at 2799.159 kb on + strand at 2799.440 kb on + strand at 2799.599 kb on + strand, within Pf6N2E2_2365 at 2799.599 kb on + strand, within Pf6N2E2_2365 at 2799.599 kb on + strand, within Pf6N2E2_2365 at 2799.605 kb on + strand, within Pf6N2E2_2365 at 2799.605 kb on + strand, within Pf6N2E2_2365 at 2799.605 kb on + strand, within Pf6N2E2_2365 at 2799.606 kb on - strand, within Pf6N2E2_2365 at 2799.768 kb on - strand, within Pf6N2E2_2365 at 2799.779 kb on + strand, within Pf6N2E2_2365 at 2799.788 kb on + strand, within Pf6N2E2_2365 at 2799.789 kb on - strand, within Pf6N2E2_2365 at 2799.789 kb on - strand, within Pf6N2E2_2365 at 2799.838 kb on - strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.860 kb on + strand, within Pf6N2E2_2365 at 2799.861 kb on - strand, within Pf6N2E2_2365 at 2799.861 kb on - strand, within Pf6N2E2_2365 at 2799.861 kb on - strand, within Pf6N2E2_2365 at 2799.864 kb on - strand, within Pf6N2E2_2365 at 2799.864 kb on - strand, within Pf6N2E2_2365 at 2800.004 kb on + strand, within Pf6N2E2_2365 at 2800.005 kb on - strand, within Pf6N2E2_2365 at 2800.370 kb on + strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.371 kb on - strand, within Pf6N2E2_2366 at 2800.384 kb on + strand, within Pf6N2E2_2366 at 2800.385 kb on - strand, within Pf6N2E2_2366 at 2800.878 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction No vitamins t1 remove 2,799,125 + -1.1 2,799,126 - -0.3 2,799,159 + -0.1 2,799,440 + +1.1 2,799,599 + Pf6N2E2_2365 0.27 -0.1 2,799,599 + Pf6N2E2_2365 0.27 -2.6 2,799,599 + Pf6N2E2_2365 0.27 -0.5 2,799,605 + Pf6N2E2_2365 0.28 +0.2 2,799,605 + Pf6N2E2_2365 0.28 -1.0 2,799,605 + Pf6N2E2_2365 0.28 +0.2 2,799,606 - Pf6N2E2_2365 0.28 +0.2 2,799,768 - Pf6N2E2_2365 0.53 +1.0 2,799,779 + Pf6N2E2_2365 0.54 +0.6 2,799,788 + Pf6N2E2_2365 0.56 -2.5 2,799,789 - Pf6N2E2_2365 0.56 -1.0 2,799,789 - Pf6N2E2_2365 0.56 +0.3 2,799,838 - Pf6N2E2_2365 0.63 -0.1 2,799,860 + Pf6N2E2_2365 0.67 -2.0 2,799,860 + Pf6N2E2_2365 0.67 -0.3 2,799,860 + Pf6N2E2_2365 0.67 -0.1 2,799,860 + Pf6N2E2_2365 0.67 -2.0 2,799,860 + Pf6N2E2_2365 0.67 -0.1 2,799,861 - Pf6N2E2_2365 0.67 +0.0 2,799,861 - Pf6N2E2_2365 0.67 -0.1 2,799,861 - Pf6N2E2_2365 0.67 -0.1 2,799,864 - Pf6N2E2_2365 0.67 -0.1 2,799,864 - Pf6N2E2_2365 0.67 -2.7 2,800,004 + Pf6N2E2_2365 0.88 -0.3 2,800,005 - Pf6N2E2_2365 0.88 -0.7 2,800,370 + Pf6N2E2_2366 0.78 +0.4 2,800,371 - Pf6N2E2_2366 0.78 -0.1 2,800,371 - Pf6N2E2_2366 0.78 +0.6 2,800,371 - Pf6N2E2_2366 0.78 +0.9 2,800,371 - Pf6N2E2_2366 0.78 -0.1 2,800,384 + Pf6N2E2_2366 0.82 -0.1 2,800,385 - Pf6N2E2_2366 0.82 +0.7 2,800,878 - -0.5
Or see this region's nucleotide sequence