Experiment: With vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_6004 and Pf6N2E2_6005 are separated by 193 nucleotides Pf6N2E2_6005 and Pf6N2E2_6170 overlap by 5 nucleotides Pf6N2E2_6170 and Pf6N2E2_6006 are separated by 325 nucleotides Pf6N2E2_6006 and Pf6N2E2_6007 are separated by 428 nucleotides
Pf6N2E2_6004 - Enoyl-CoA hydratase/isomerase family protein, at 6,821,888 to 6,822,577
_6004
Pf6N2E2_6005 - hypothetical protein, at 6,822,771 to 6,822,890
_6005
Pf6N2E2_6170 - tRNA-Ser-GGA, at 6,822,886 to 6,822,972
_6170
Pf6N2E2_6006 - L-rhamnose operon transcriptional activator RhaR, at 6,823,298 to 6,823,867
_6006
Pf6N2E2_6007 - Glutathione S-transferase (EC 2.5.1.18), at 6,824,296 to 6,824,850
_6007
Position (kb)
6823
6824 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 6822.559 kb on - strand at 6822.584 kb on - strand at 6822.592 kb on + strand at 6822.593 kb on - strand at 6822.593 kb on - strand at 6822.593 kb on - strand at 6822.593 kb on - strand at 6822.593 kb on - strand at 6822.593 kb on - strand at 6822.634 kb on + strand at 6822.635 kb on - strand at 6822.635 kb on - strand at 6822.713 kb on + strand at 6822.714 kb on - strand at 6822.714 kb on - strand at 6822.714 kb on - strand at 6822.714 kb on - strand at 6822.715 kb on + strand at 6822.715 kb on + strand at 6822.716 kb on - strand at 6823.114 kb on - strand at 6823.122 kb on + strand at 6823.156 kb on + strand at 6823.156 kb on + strand at 6823.156 kb on + strand at 6823.156 kb on + strand at 6823.157 kb on - strand at 6823.157 kb on - strand at 6823.157 kb on - strand at 6823.287 kb on + strand at 6823.288 kb on - strand at 6823.291 kb on + strand at 6823.291 kb on + strand at 6823.291 kb on + strand at 6823.465 kb on - strand, within Pf6N2E2_6006 at 6823.465 kb on - strand, within Pf6N2E2_6006 at 6823.465 kb on - strand, within Pf6N2E2_6006 at 6823.556 kb on + strand, within Pf6N2E2_6006 at 6823.556 kb on + strand, within Pf6N2E2_6006 at 6823.556 kb on + strand, within Pf6N2E2_6006 at 6823.557 kb on - strand, within Pf6N2E2_6006 at 6823.557 kb on - strand, within Pf6N2E2_6006 at 6823.684 kb on + strand, within Pf6N2E2_6006 at 6823.684 kb on + strand, within Pf6N2E2_6006 at 6823.684 kb on + strand, within Pf6N2E2_6006 at 6823.792 kb on + strand, within Pf6N2E2_6006 at 6823.793 kb on - strand, within Pf6N2E2_6006 at 6823.852 kb on - strand at 6823.863 kb on + strand at 6823.863 kb on + strand at 6823.947 kb on + strand at 6823.948 kb on - strand at 6823.990 kb on + strand at 6824.055 kb on - strand at 6824.165 kb on + strand at 6824.170 kb on - strand at 6824.193 kb on - strand at 6824.199 kb on + strand at 6824.232 kb on + strand at 6824.446 kb on + strand, within Pf6N2E2_6007 at 6824.446 kb on + strand, within Pf6N2E2_6007 at 6824.446 kb on + strand, within Pf6N2E2_6007 at 6824.446 kb on + strand, within Pf6N2E2_6007 at 6824.447 kb on - strand, within Pf6N2E2_6007 at 6824.447 kb on - strand, within Pf6N2E2_6007 at 6824.644 kb on - strand, within Pf6N2E2_6007 at 6824.783 kb on - strand, within Pf6N2E2_6007
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t2 remove 6,822,559 - -0.1 6,822,584 - -1.7 6,822,592 + -0.2 6,822,593 - +1.2 6,822,593 - -1.7 6,822,593 - +0.5 6,822,593 - -0.3 6,822,593 - +1.9 6,822,593 - +0.8 6,822,634 + +0.7 6,822,635 - -2.1 6,822,635 - +1.3 6,822,713 + +0.8 6,822,714 - -0.1 6,822,714 - -0.1 6,822,714 - -1.7 6,822,714 - -2.1 6,822,715 + -1.1 6,822,715 + -1.4 6,822,716 - -0.1 6,823,114 - -0.9 6,823,122 + -0.1 6,823,156 + +0.3 6,823,156 + +0.1 6,823,156 + -0.1 6,823,156 + +0.3 6,823,157 - -0.7 6,823,157 - +0.1 6,823,157 - -1.5 6,823,287 + -1.3 6,823,288 - -0.1 6,823,291 + -0.8 6,823,291 + -0.1 6,823,291 + -0.1 6,823,465 - Pf6N2E2_6006 0.29 -0.8 6,823,465 - Pf6N2E2_6006 0.29 +0.9 6,823,465 - Pf6N2E2_6006 0.29 -0.1 6,823,556 + Pf6N2E2_6006 0.45 -0.1 6,823,556 + Pf6N2E2_6006 0.45 -0.1 6,823,556 + Pf6N2E2_6006 0.45 -0.6 6,823,557 - Pf6N2E2_6006 0.45 -0.1 6,823,557 - Pf6N2E2_6006 0.45 -1.4 6,823,684 + Pf6N2E2_6006 0.68 -1.7 6,823,684 + Pf6N2E2_6006 0.68 -0.1 6,823,684 + Pf6N2E2_6006 0.68 -1.1 6,823,792 + Pf6N2E2_6006 0.87 -0.7 6,823,793 - Pf6N2E2_6006 0.87 -1.1 6,823,852 - -0.1 6,823,863 + +0.6 6,823,863 + -0.1 6,823,947 + -1.1 6,823,948 - -1.1 6,823,990 + -0.1 6,824,055 - +2.2 6,824,165 + -0.1 6,824,170 - -0.1 6,824,193 - -0.1 6,824,199 + -1.7 6,824,232 + -0.1 6,824,446 + Pf6N2E2_6007 0.27 -0.7 6,824,446 + Pf6N2E2_6007 0.27 -0.1 6,824,446 + Pf6N2E2_6007 0.27 -0.1 6,824,446 + Pf6N2E2_6007 0.27 +0.3 6,824,447 - Pf6N2E2_6007 0.27 -0.3 6,824,447 - Pf6N2E2_6007 0.27 -0.1 6,824,644 - Pf6N2E2_6007 0.63 -0.1 6,824,783 - Pf6N2E2_6007 0.88 -2.4
Or see this region's nucleotide sequence