Experiment: With vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1600 and Pf6N2E2_1601 are separated by 5 nucleotides Pf6N2E2_1601 and Pf6N2E2_1602 are separated by 70 nucleotides
Pf6N2E2_1600 - diguanylate cyclase/phosphodiesterase (GGDEF & EAL domains) with PAS/PAC sensor(s), at 1,872,220 to 1,873,872
_1600
Pf6N2E2_1601 - DNA-binding response regulator, LuxR family, at 1,873,878 to 1,874,459
_1601
Pf6N2E2_1602 - hypothetical protein, at 1,874,530 to 1,875,312
_1602
Position (kb)
1873
1874
1875 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1872.982 kb on + strand, within Pf6N2E2_1600 at 1873.103 kb on + strand, within Pf6N2E2_1600 at 1873.103 kb on + strand, within Pf6N2E2_1600 at 1873.104 kb on - strand, within Pf6N2E2_1600 at 1873.229 kb on + strand, within Pf6N2E2_1600 at 1873.397 kb on - strand, within Pf6N2E2_1600 at 1873.514 kb on + strand, within Pf6N2E2_1600 at 1873.595 kb on + strand, within Pf6N2E2_1600 at 1873.595 kb on + strand, within Pf6N2E2_1600 at 1873.596 kb on - strand, within Pf6N2E2_1600 at 1873.889 kb on + strand at 1873.890 kb on - strand at 1874.285 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.340 kb on + strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.341 kb on - strand, within Pf6N2E2_1601 at 1874.343 kb on - strand, within Pf6N2E2_1601 at 1874.391 kb on + strand, within Pf6N2E2_1601 at 1874.391 kb on + strand, within Pf6N2E2_1601 at 1874.392 kb on - strand, within Pf6N2E2_1601 at 1874.508 kb on - strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.543 kb on + strand at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.766 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.768 kb on + strand, within Pf6N2E2_1602 at 1874.831 kb on + strand, within Pf6N2E2_1602 at 1874.936 kb on + strand, within Pf6N2E2_1602 at 1875.005 kb on + strand, within Pf6N2E2_1602 at 1875.154 kb on - strand, within Pf6N2E2_1602 at 1875.402 kb on - strand at 1875.402 kb on - strand at 1875.402 kb on - strand at 1875.404 kb on - strand at 1875.418 kb on - strand at 1875.419 kb on + strand at 1875.444 kb on + strand at 1875.445 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t2 remove 1,872,982 + Pf6N2E2_1600 0.46 -1.3 1,873,103 + Pf6N2E2_1600 0.53 +0.0 1,873,103 + Pf6N2E2_1600 0.53 +0.1 1,873,104 - Pf6N2E2_1600 0.53 -1.3 1,873,229 + Pf6N2E2_1600 0.61 -0.5 1,873,397 - Pf6N2E2_1600 0.71 -1.5 1,873,514 + Pf6N2E2_1600 0.78 -1.5 1,873,595 + Pf6N2E2_1600 0.83 +0.3 1,873,595 + Pf6N2E2_1600 0.83 +0.5 1,873,596 - Pf6N2E2_1600 0.83 +0.5 1,873,889 + -0.5 1,873,890 - +0.9 1,874,285 + Pf6N2E2_1601 0.70 -0.5 1,874,340 + Pf6N2E2_1601 0.79 +1.2 1,874,340 + Pf6N2E2_1601 0.79 +0.8 1,874,340 + Pf6N2E2_1601 0.79 +2.0 1,874,341 - Pf6N2E2_1601 0.80 +2.0 1,874,341 - Pf6N2E2_1601 0.80 +1.0 1,874,341 - Pf6N2E2_1601 0.80 -0.5 1,874,341 - Pf6N2E2_1601 0.80 -0.5 1,874,341 - Pf6N2E2_1601 0.80 +1.5 1,874,341 - Pf6N2E2_1601 0.80 +0.9 1,874,341 - Pf6N2E2_1601 0.80 -0.5 1,874,341 - Pf6N2E2_1601 0.80 +0.0 1,874,341 - Pf6N2E2_1601 0.80 +0.7 1,874,343 - Pf6N2E2_1601 0.80 +1.3 1,874,391 + Pf6N2E2_1601 0.88 -0.5 1,874,391 + Pf6N2E2_1601 0.88 +0.5 1,874,392 - Pf6N2E2_1601 0.88 -0.0 1,874,508 - +0.6 1,874,543 + -1.1 1,874,543 + +0.7 1,874,543 + +0.5 1,874,766 + Pf6N2E2_1602 0.30 -0.5 1,874,766 + Pf6N2E2_1602 0.30 +0.5 1,874,768 + Pf6N2E2_1602 0.30 -0.1 1,874,768 + Pf6N2E2_1602 0.30 +1.2 1,874,831 + Pf6N2E2_1602 0.38 -1.5 1,874,936 + Pf6N2E2_1602 0.52 +0.7 1,875,005 + Pf6N2E2_1602 0.61 +1.5 1,875,154 - Pf6N2E2_1602 0.80 -1.3 1,875,402 - +0.9 1,875,402 - -1.2 1,875,402 - -2.3 1,875,404 - +0.5 1,875,418 - +1.5 1,875,419 + -1.5 1,875,444 + +0.2 1,875,445 - +1.3
Or see this region's nucleotide sequence