Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1542

Experiment: With vitamins t2

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1540 and Pf6N2E2_1541 overlap by 4 nucleotidesPf6N2E2_1541 and Pf6N2E2_1542 overlap by 14 nucleotidesPf6N2E2_1542 and Pf6N2E2_1543 overlap by 4 nucleotides Pf6N2E2_1540 - FIG00953528: hypothetical protein, at 1,799,130 to 1,799,774 _1540 Pf6N2E2_1541 - Short-chain dehydrogenase/reductase SDR, at 1,799,771 to 1,800,580 _1541 Pf6N2E2_1542 - long-chain acyl-CoA synthetase, at 1,800,567 to 1,801,241 _1542 Pf6N2E2_1543 - Long-chain-fatty-acid--CoA ligase (EC 6.2.1.3), at 1,801,238 to 1,802,719 _1543 Position (kb) 1800 1801 1802Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1799.657 kb on - strand, within Pf6N2E2_1540at 1799.663 kb on + strand, within Pf6N2E2_1540at 1799.663 kb on + strand, within Pf6N2E2_1540at 1799.907 kb on + strand, within Pf6N2E2_1541at 1799.908 kb on - strand, within Pf6N2E2_1541at 1799.908 kb on - strand, within Pf6N2E2_1541at 1799.908 kb on - strand, within Pf6N2E2_1541at 1800.047 kb on + strand, within Pf6N2E2_1541at 1800.048 kb on - strand, within Pf6N2E2_1541at 1800.051 kb on + strand, within Pf6N2E2_1541at 1800.051 kb on + strand, within Pf6N2E2_1541at 1800.051 kb on + strand, within Pf6N2E2_1541at 1800.051 kb on + strand, within Pf6N2E2_1541at 1800.051 kb on + strand, within Pf6N2E2_1541at 1800.051 kb on + strand, within Pf6N2E2_1541at 1800.052 kb on - strand, within Pf6N2E2_1541at 1800.052 kb on - strand, within Pf6N2E2_1541at 1800.052 kb on - strand, within Pf6N2E2_1541at 1800.052 kb on - strand, within Pf6N2E2_1541at 1800.052 kb on - strand, within Pf6N2E2_1541at 1800.132 kb on + strand, within Pf6N2E2_1541at 1800.165 kb on + strand, within Pf6N2E2_1541at 1800.166 kb on - strand, within Pf6N2E2_1541at 1800.166 kb on - strand, within Pf6N2E2_1541at 1800.375 kb on - strand, within Pf6N2E2_1541at 1800.706 kb on + strand, within Pf6N2E2_1542at 1800.716 kb on + strand, within Pf6N2E2_1542at 1800.716 kb on + strand, within Pf6N2E2_1542at 1800.717 kb on - strand, within Pf6N2E2_1542at 1800.717 kb on - strand, within Pf6N2E2_1542at 1800.751 kb on + strand, within Pf6N2E2_1542at 1800.889 kb on + strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1800.896 kb on - strand, within Pf6N2E2_1542at 1801.001 kb on - strand, within Pf6N2E2_1542at 1801.015 kb on + strand, within Pf6N2E2_1542at 1801.015 kb on + strand, within Pf6N2E2_1542at 1801.015 kb on + strand, within Pf6N2E2_1542at 1801.106 kb on - strand, within Pf6N2E2_1542at 1801.106 kb on - strand, within Pf6N2E2_1542at 1801.108 kb on + strand, within Pf6N2E2_1542at 1801.108 kb on + strand, within Pf6N2E2_1542at 1801.109 kb on - strand, within Pf6N2E2_1542at 1801.503 kb on + strand, within Pf6N2E2_1543at 1801.618 kb on - strand, within Pf6N2E2_1543at 1802.115 kb on + strand, within Pf6N2E2_1543at 1802.115 kb on + strand, within Pf6N2E2_1543at 1802.116 kb on - strand, within Pf6N2E2_1543at 1802.116 kb on - strand, within Pf6N2E2_1543at 1802.127 kb on + strand, within Pf6N2E2_1543at 1802.128 kb on - strand, within Pf6N2E2_1543at 1802.166 kb on - strand, within Pf6N2E2_1543

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Per-strain Table

Position Strand Gene LocusTag Fraction With vitamins t2
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1,799,657 - Pf6N2E2_1540 0.82 +1.0
1,799,663 + Pf6N2E2_1540 0.83 +0.7
1,799,663 + Pf6N2E2_1540 0.83 +0.7
1,799,907 + Pf6N2E2_1541 0.17 +0.4
1,799,908 - Pf6N2E2_1541 0.17 -1.3
1,799,908 - Pf6N2E2_1541 0.17 +1.0
1,799,908 - Pf6N2E2_1541 0.17 -1.6
1,800,047 + Pf6N2E2_1541 0.34 -1.6
1,800,048 - Pf6N2E2_1541 0.34 -0.1
1,800,051 + Pf6N2E2_1541 0.35 +1.4
1,800,051 + Pf6N2E2_1541 0.35 +0.8
1,800,051 + Pf6N2E2_1541 0.35 -0.2
1,800,051 + Pf6N2E2_1541 0.35 +1.2
1,800,051 + Pf6N2E2_1541 0.35 -0.2
1,800,051 + Pf6N2E2_1541 0.35 +1.0
1,800,052 - Pf6N2E2_1541 0.35 +3.1
1,800,052 - Pf6N2E2_1541 0.35 -0.9
1,800,052 - Pf6N2E2_1541 0.35 +2.4
1,800,052 - Pf6N2E2_1541 0.35 -0.6
1,800,052 - Pf6N2E2_1541 0.35 +0.2
1,800,132 + Pf6N2E2_1541 0.45 -0.6
1,800,165 + Pf6N2E2_1541 0.49 +1.4
1,800,166 - Pf6N2E2_1541 0.49 +0.2
1,800,166 - Pf6N2E2_1541 0.49 +1.0
1,800,375 - Pf6N2E2_1541 0.75 +0.0
1,800,706 + Pf6N2E2_1542 0.21 -2.1
1,800,716 + Pf6N2E2_1542 0.22 +1.4
1,800,716 + Pf6N2E2_1542 0.22 +0.4
1,800,717 - Pf6N2E2_1542 0.22 +1.1
1,800,717 - Pf6N2E2_1542 0.22 +2.2
1,800,751 + Pf6N2E2_1542 0.27 +1.0
1,800,889 + Pf6N2E2_1542 0.48 -1.6
1,800,896 - Pf6N2E2_1542 0.49 +0.3
1,800,896 - Pf6N2E2_1542 0.49 -0.6
1,800,896 - Pf6N2E2_1542 0.49 -0.6
1,800,896 - Pf6N2E2_1542 0.49 +0.2
1,800,896 - Pf6N2E2_1542 0.49 -0.6
1,800,896 - Pf6N2E2_1542 0.49 -0.6
1,801,001 - Pf6N2E2_1542 0.64 +2.1
1,801,015 + Pf6N2E2_1542 0.66 +0.7
1,801,015 + Pf6N2E2_1542 0.66 -1.6
1,801,015 + Pf6N2E2_1542 0.66 +0.0
1,801,106 - Pf6N2E2_1542 0.80 +1.8
1,801,106 - Pf6N2E2_1542 0.80 +0.4
1,801,108 + Pf6N2E2_1542 0.80 +0.8
1,801,108 + Pf6N2E2_1542 0.80 -0.6
1,801,109 - Pf6N2E2_1542 0.80 +1.0
1,801,503 + Pf6N2E2_1543 0.18 -0.6
1,801,618 - Pf6N2E2_1543 0.26 +1.0
1,802,115 + Pf6N2E2_1543 0.59 +0.4
1,802,115 + Pf6N2E2_1543 0.59 -2.2
1,802,116 - Pf6N2E2_1543 0.59 -0.9
1,802,116 - Pf6N2E2_1543 0.59 +1.1
1,802,127 + Pf6N2E2_1543 0.60 -0.6
1,802,128 - Pf6N2E2_1543 0.60 +0.8
1,802,166 - Pf6N2E2_1543 0.63 +0.2

Or see this region's nucleotide sequence