Experiment: With vitamins t2
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1202 and Pf6N2E2_1203 are separated by 139 nucleotides Pf6N2E2_1203 and Pf6N2E2_1204 are separated by 12 nucleotides Pf6N2E2_1204 and Pf6N2E2_1205 are separated by 46 nucleotides
Pf6N2E2_1202 - hypothetical protein, at 1,404,775 to 1,408,689
_1202
Pf6N2E2_1203 - Isoquinoline 1-oxidoreductase alpha subunit (EC 1.3.99.16), at 1,408,829 to 1,409,302
_1203
Pf6N2E2_1204 - Isoquinoline 1-oxidoreductase beta subunit (EC 1.3.99.16), at 1,409,315 to 1,411,552
_1204
Pf6N2E2_1205 - Transcriptional regulator, AraC family, at 1,411,599 to 1,412,507
_1205
Position (kb)
1409
1410
1411
1412 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1408.436 kb on - strand at 1408.436 kb on - strand at 1408.441 kb on + strand at 1408.442 kb on - strand at 1408.703 kb on + strand at 1408.780 kb on + strand at 1408.780 kb on + strand at 1408.788 kb on + strand at 1408.789 kb on - strand at 1408.789 kb on - strand at 1408.790 kb on + strand at 1408.829 kb on - strand at 1409.252 kb on + strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.253 kb on - strand, within Pf6N2E2_1203 at 1409.687 kb on + strand, within Pf6N2E2_1204 at 1409.748 kb on - strand, within Pf6N2E2_1204 at 1409.748 kb on - strand, within Pf6N2E2_1204 at 1410.139 kb on - strand, within Pf6N2E2_1204 at 1410.203 kb on + strand, within Pf6N2E2_1204 at 1410.204 kb on - strand, within Pf6N2E2_1204 at 1410.516 kb on + strand, within Pf6N2E2_1204 at 1410.704 kb on + strand, within Pf6N2E2_1204 at 1410.705 kb on - strand, within Pf6N2E2_1204 at 1410.762 kb on + strand, within Pf6N2E2_1204 at 1410.763 kb on - strand, within Pf6N2E2_1204 at 1410.869 kb on - strand, within Pf6N2E2_1204 at 1411.100 kb on + strand, within Pf6N2E2_1204 at 1411.176 kb on - strand, within Pf6N2E2_1204 at 1411.516 kb on + strand at 1411.533 kb on + strand at 1411.534 kb on - strand at 1411.654 kb on + strand at 1411.655 kb on - strand at 1411.753 kb on - strand, within Pf6N2E2_1205 at 1411.753 kb on - strand, within Pf6N2E2_1205 at 1411.771 kb on + strand, within Pf6N2E2_1205 at 1411.860 kb on - strand, within Pf6N2E2_1205 at 1411.860 kb on - strand, within Pf6N2E2_1205 at 1412.091 kb on + strand, within Pf6N2E2_1205 at 1412.091 kb on + strand, within Pf6N2E2_1205 at 1412.279 kb on - strand, within Pf6N2E2_1205 at 1412.286 kb on + strand, within Pf6N2E2_1205 at 1412.347 kb on + strand, within Pf6N2E2_1205 at 1412.347 kb on + strand, within Pf6N2E2_1205 at 1412.348 kb on - strand, within Pf6N2E2_1205 at 1412.348 kb on - strand, within Pf6N2E2_1205
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t2 remove 1,408,436 - +2.2 1,408,436 - -0.6 1,408,441 + -2.6 1,408,442 - -0.6 1,408,703 + +1.7 1,408,780 + +0.4 1,408,780 + -1.6 1,408,788 + +0.6 1,408,789 - -0.6 1,408,789 - -1.6 1,408,790 + +0.7 1,408,829 - +0.0 1,409,252 + Pf6N2E2_1203 0.89 +0.4 1,409,253 - Pf6N2E2_1203 0.89 -0.6 1,409,253 - Pf6N2E2_1203 0.89 -0.8 1,409,253 - Pf6N2E2_1203 0.89 -0.6 1,409,253 - Pf6N2E2_1203 0.89 +0.2 1,409,253 - Pf6N2E2_1203 0.89 +0.0 1,409,687 + Pf6N2E2_1204 0.17 -0.6 1,409,748 - Pf6N2E2_1204 0.19 +0.4 1,409,748 - Pf6N2E2_1204 0.19 +1.0 1,410,139 - Pf6N2E2_1204 0.37 -0.0 1,410,203 + Pf6N2E2_1204 0.40 -0.9 1,410,204 - Pf6N2E2_1204 0.40 +2.9 1,410,516 + Pf6N2E2_1204 0.54 +1.7 1,410,704 + Pf6N2E2_1204 0.62 -0.6 1,410,705 - Pf6N2E2_1204 0.62 -0.6 1,410,762 + Pf6N2E2_1204 0.65 +0.1 1,410,763 - Pf6N2E2_1204 0.65 +0.2 1,410,869 - Pf6N2E2_1204 0.69 +0.4 1,411,100 + Pf6N2E2_1204 0.80 +0.6 1,411,176 - Pf6N2E2_1204 0.83 -0.6 1,411,516 + +0.7 1,411,533 + +0.8 1,411,534 - -1.6 1,411,654 + -0.3 1,411,655 - +0.4 1,411,753 - Pf6N2E2_1205 0.17 -0.9 1,411,753 - Pf6N2E2_1205 0.17 -0.0 1,411,771 + Pf6N2E2_1205 0.19 -0.6 1,411,860 - Pf6N2E2_1205 0.29 -0.6 1,411,860 - Pf6N2E2_1205 0.29 -0.6 1,412,091 + Pf6N2E2_1205 0.54 -0.2 1,412,091 + Pf6N2E2_1205 0.54 +2.0 1,412,279 - Pf6N2E2_1205 0.75 -0.0 1,412,286 + Pf6N2E2_1205 0.76 +2.4 1,412,347 + Pf6N2E2_1205 0.82 +0.1 1,412,347 + Pf6N2E2_1205 0.82 -1.0 1,412,348 - Pf6N2E2_1205 0.82 -0.1 1,412,348 - Pf6N2E2_1205 0.82 +0.7
Or see this region's nucleotide sequence