Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_295

Experiment: With vitamins t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_293 and Pf6N2E2_294 are separated by 123 nucleotidesPf6N2E2_294 and Pf6N2E2_295 are separated by 57 nucleotidesPf6N2E2_295 and Pf6N2E2_296 are separated by 81 nucleotides Pf6N2E2_293 - Translation initiation factor 1, at 363,075 to 363,293 _293 Pf6N2E2_294 - Arginine-tRNA-protein transferase (EC 2.3.2.8), at 363,417 to 364,124 _294 Pf6N2E2_295 - Leucyl/phenylalanyl-tRNA--protein transferase (EC 2.3.2.6), at 364,182 to 364,862 _295 Pf6N2E2_296 - Thioredoxin reductase (EC 1.8.1.9), at 364,944 to 365,903 _296 Position (kb) 364 365Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 363.396 kb on - strandat 363.396 kb on - strandat 363.402 kb on - strandat 363.402 kb on - strandat 363.402 kb on - strandat 363.404 kb on - strandat 363.479 kb on - strandat 363.517 kb on + strand, within Pf6N2E2_294at 363.518 kb on - strand, within Pf6N2E2_294at 363.518 kb on - strand, within Pf6N2E2_294at 363.608 kb on - strand, within Pf6N2E2_294at 363.608 kb on - strand, within Pf6N2E2_294at 363.608 kb on - strand, within Pf6N2E2_294at 363.616 kb on + strand, within Pf6N2E2_294at 363.616 kb on + strand, within Pf6N2E2_294at 363.616 kb on + strand, within Pf6N2E2_294at 363.617 kb on - strand, within Pf6N2E2_294at 363.692 kb on - strand, within Pf6N2E2_294at 363.692 kb on - strand, within Pf6N2E2_294at 363.754 kb on + strand, within Pf6N2E2_294at 363.754 kb on + strand, within Pf6N2E2_294at 363.754 kb on + strand, within Pf6N2E2_294at 363.755 kb on - strand, within Pf6N2E2_294at 363.755 kb on - strand, within Pf6N2E2_294at 363.787 kb on + strand, within Pf6N2E2_294at 363.787 kb on + strand, within Pf6N2E2_294at 363.787 kb on + strand, within Pf6N2E2_294at 363.787 kb on + strand, within Pf6N2E2_294at 363.788 kb on - strand, within Pf6N2E2_294at 363.797 kb on - strand, within Pf6N2E2_294at 363.808 kb on + strand, within Pf6N2E2_294at 363.808 kb on + strand, within Pf6N2E2_294at 363.809 kb on - strand, within Pf6N2E2_294at 363.809 kb on - strand, within Pf6N2E2_294at 363.972 kb on - strand, within Pf6N2E2_294at 364.097 kb on - strandat 364.147 kb on + strandat 364.148 kb on - strandat 364.153 kb on + strandat 364.153 kb on + strandat 364.153 kb on + strandat 364.154 kb on - strandat 364.159 kb on + strandat 364.159 kb on + strandat 364.160 kb on - strandat 364.160 kb on - strandat 364.160 kb on - strandat 364.231 kb on + strandat 364.232 kb on - strandat 364.419 kb on + strand, within Pf6N2E2_295at 364.427 kb on - strand, within Pf6N2E2_295at 364.544 kb on - strand, within Pf6N2E2_295at 364.606 kb on + strand, within Pf6N2E2_295at 364.606 kb on + strand, within Pf6N2E2_295at 364.768 kb on - strand, within Pf6N2E2_295at 364.867 kb on - strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.878 kb on + strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.879 kb on - strandat 364.969 kb on + strandat 364.969 kb on + strandat 364.969 kb on + strandat 364.969 kb on + strandat 364.970 kb on - strandat 364.970 kb on - strandat 364.970 kb on - strandat 364.970 kb on - strandat 365.015 kb on + strandat 365.015 kb on + strandat 365.015 kb on + strandat 365.016 kb on - strandat 365.016 kb on - strandat 365.016 kb on - strandat 365.110 kb on - strand, within Pf6N2E2_296at 365.151 kb on + strand, within Pf6N2E2_296at 365.152 kb on - strand, within Pf6N2E2_296at 365.169 kb on - strand, within Pf6N2E2_296at 365.169 kb on - strand, within Pf6N2E2_296at 365.177 kb on + strand, within Pf6N2E2_296at 365.187 kb on + strand, within Pf6N2E2_296at 365.306 kb on + strand, within Pf6N2E2_296at 365.307 kb on - strand, within Pf6N2E2_296at 365.413 kb on + strand, within Pf6N2E2_296at 365.413 kb on + strand, within Pf6N2E2_296at 365.413 kb on + strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.414 kb on - strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.593 kb on + strand, within Pf6N2E2_296at 365.594 kb on - strand, within Pf6N2E2_296at 365.652 kb on - strand, within Pf6N2E2_296at 365.652 kb on - strand, within Pf6N2E2_296at 365.712 kb on + strand, within Pf6N2E2_296at 365.833 kb on + strandat 365.833 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction With vitamins t1
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363,396 - -0.6
363,396 - -0.3
363,402 - +0.7
363,402 - -0.3
363,402 - -1.3
363,404 - +0.4
363,479 - -1.6
363,517 + Pf6N2E2_294 0.14 -0.7
363,518 - Pf6N2E2_294 0.14 +0.1
363,518 - Pf6N2E2_294 0.14 +0.1
363,608 - Pf6N2E2_294 0.27 -0.3
363,608 - Pf6N2E2_294 0.27 +0.4
363,608 - Pf6N2E2_294 0.27 -0.3
363,616 + Pf6N2E2_294 0.28 -1.9
363,616 + Pf6N2E2_294 0.28 -0.9
363,616 + Pf6N2E2_294 0.28 -0.3
363,617 - Pf6N2E2_294 0.28 -0.3
363,692 - Pf6N2E2_294 0.39 +0.9
363,692 - Pf6N2E2_294 0.39 -0.8
363,754 + Pf6N2E2_294 0.48 -0.3
363,754 + Pf6N2E2_294 0.48 -1.9
363,754 + Pf6N2E2_294 0.48 -0.1
363,755 - Pf6N2E2_294 0.48 -0.3
363,755 - Pf6N2E2_294 0.48 -0.3
363,787 + Pf6N2E2_294 0.52 -0.5
363,787 + Pf6N2E2_294 0.52 -0.5
363,787 + Pf6N2E2_294 0.52 -0.3
363,787 + Pf6N2E2_294 0.52 -1.3
363,788 - Pf6N2E2_294 0.52 -1.9
363,797 - Pf6N2E2_294 0.54 +0.7
363,808 + Pf6N2E2_294 0.55 -1.1
363,808 + Pf6N2E2_294 0.55 -0.3
363,809 - Pf6N2E2_294 0.55 +0.4
363,809 - Pf6N2E2_294 0.55 -0.3
363,972 - Pf6N2E2_294 0.78 -0.3
364,097 - +0.9
364,147 + +0.7
364,148 - -0.3
364,153 + -1.9
364,153 + -1.9
364,153 + -1.9
364,154 - -1.3
364,159 + -0.7
364,159 + -0.0
364,160 - -1.9
364,160 - -0.3
364,160 - -0.3
364,231 + +0.7
364,232 - -0.3
364,419 + Pf6N2E2_295 0.35 -1.9
364,427 - Pf6N2E2_295 0.36 -1.9
364,544 - Pf6N2E2_295 0.53 -0.6
364,606 + Pf6N2E2_295 0.62 +0.7
364,606 + Pf6N2E2_295 0.62 -0.3
364,768 - Pf6N2E2_295 0.86 -2.5
364,867 - +0.2
364,878 + -0.3
364,878 + +1.1
364,878 + +0.5
364,878 + +0.6
364,878 + -0.3
364,879 - -0.3
364,879 - +0.0
364,879 - -0.8
364,879 - -0.3
364,879 - -0.1
364,879 - -3.1
364,879 - +0.7
364,969 + -2.6
364,969 + -0.3
364,969 + +1.3
364,969 + +0.1
364,970 - -0.3
364,970 - -1.5
364,970 - -0.3
364,970 - -0.1
365,015 + +0.0
365,015 + -0.8
365,015 + -0.3
365,016 - +1.7
365,016 - +0.0
365,016 - -0.3
365,110 - Pf6N2E2_296 0.17 -0.1
365,151 + Pf6N2E2_296 0.22 -1.9
365,152 - Pf6N2E2_296 0.22 +2.3
365,169 - Pf6N2E2_296 0.23 +0.7
365,169 - Pf6N2E2_296 0.23 +1.0
365,177 + Pf6N2E2_296 0.24 -0.3
365,187 + Pf6N2E2_296 0.25 +0.4
365,306 + Pf6N2E2_296 0.38 +0.4
365,307 - Pf6N2E2_296 0.38 -0.5
365,413 + Pf6N2E2_296 0.49 -0.1
365,413 + Pf6N2E2_296 0.49 -0.3
365,413 + Pf6N2E2_296 0.49 +0.3
365,414 - Pf6N2E2_296 0.49 -1.3
365,414 - Pf6N2E2_296 0.49 +0.3
365,414 - Pf6N2E2_296 0.49 +1.3
365,414 - Pf6N2E2_296 0.49 -0.3
365,414 - Pf6N2E2_296 0.49 +1.7
365,414 - Pf6N2E2_296 0.49 -0.3
365,414 - Pf6N2E2_296 0.49 +0.1
365,414 - Pf6N2E2_296 0.49 +0.4
365,414 - Pf6N2E2_296 0.49 +0.3
365,593 + Pf6N2E2_296 0.68 +1.3
365,593 + Pf6N2E2_296 0.68 +0.4
365,593 + Pf6N2E2_296 0.68 -1.3
365,593 + Pf6N2E2_296 0.68 -0.3
365,593 + Pf6N2E2_296 0.68 -0.3
365,593 + Pf6N2E2_296 0.68 -1.0
365,594 - Pf6N2E2_296 0.68 -0.9
365,652 - Pf6N2E2_296 0.74 -0.9
365,652 - Pf6N2E2_296 0.74 -0.3
365,712 + Pf6N2E2_296 0.80 -1.1
365,833 + +1.3
365,833 + -0.3

Or see this region's nucleotide sequence