Experiment: With vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_2298 and Pf6N2E2_2299 overlap by 4 nucleotides Pf6N2E2_2299 and Pf6N2E2_2300 are separated by 187 nucleotides Pf6N2E2_2300 and Pf6N2E2_2301 are separated by 172 nucleotides
Pf6N2E2_2298 - Multi antimicrobial extrusion protein (Na(+)/drug antiporter), MATE family of MDR efflux pumps, at 2,721,089 to 2,722,423
_2298
Pf6N2E2_2299 - tRNA 5-methylaminomethyl-2-thiouridine synthase TusA, at 2,722,420 to 2,722,671
_2299
Pf6N2E2_2300 - LSU rRNA 2'-O-methyl-C2498 methyltransferase RlmM, at 2,722,859 to 2,723,932
_2300
Pf6N2E2_2301 - Aconitate hydratase (EC 4.2.1.3), at 2,724,105 to 2,726,846
_2301
Position (kb)
2722
2723
2724 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2721.920 kb on + strand, within Pf6N2E2_2298 at 2722.029 kb on - strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.085 kb on + strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.086 kb on - strand, within Pf6N2E2_2298 at 2722.134 kb on - strand, within Pf6N2E2_2298 at 2722.677 kb on - strand at 2722.677 kb on - strand at 2722.926 kb on + strand at 2722.927 kb on - strand at 2722.927 kb on - strand at 2723.794 kb on + strand, within Pf6N2E2_2300 at 2723.794 kb on + strand, within Pf6N2E2_2300 at 2723.795 kb on - strand, within Pf6N2E2_2300 at 2724.005 kb on + strand at 2724.025 kb on + strand at 2724.025 kb on + strand at 2724.025 kb on + strand at 2724.025 kb on + strand at 2724.026 kb on - strand at 2724.026 kb on - strand at 2724.042 kb on + strand at 2724.042 kb on + strand at 2724.043 kb on - strand at 2724.089 kb on + strand at 2724.089 kb on + strand at 2724.089 kb on + strand at 2724.089 kb on + strand at 2724.090 kb on - strand at 2724.090 kb on - strand at 2724.162 kb on + strand at 2724.162 kb on + strand at 2724.162 kb on + strand at 2724.163 kb on - strand at 2724.163 kb on - strand at 2724.163 kb on - strand at 2724.168 kb on + strand at 2724.168 kb on + strand at 2724.168 kb on + strand at 2724.168 kb on + strand at 2724.168 kb on + strand at 2724.169 kb on - strand at 2724.169 kb on - strand at 2724.345 kb on + strand at 2724.574 kb on - strand, within Pf6N2E2_2301 at 2724.663 kb on + strand, within Pf6N2E2_2301 at 2724.877 kb on + strand, within Pf6N2E2_2301
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t1 remove 2,721,920 + Pf6N2E2_2298 0.62 +0.9 2,722,029 - Pf6N2E2_2298 0.70 +0.4 2,722,085 + Pf6N2E2_2298 0.75 -1.7 2,722,085 + Pf6N2E2_2298 0.75 -1.6 2,722,085 + Pf6N2E2_2298 0.75 +0.4 2,722,086 - Pf6N2E2_2298 0.75 +0.4 2,722,086 - Pf6N2E2_2298 0.75 -0.1 2,722,086 - Pf6N2E2_2298 0.75 -0.1 2,722,086 - Pf6N2E2_2298 0.75 -0.1 2,722,086 - Pf6N2E2_2298 0.75 -1.3 2,722,134 - Pf6N2E2_2298 0.78 +1.9 2,722,677 - -0.8 2,722,677 - +1.4 2,722,926 + -1.1 2,722,927 - -0.2 2,722,927 - +0.3 2,723,794 + Pf6N2E2_2300 0.87 -0.1 2,723,794 + Pf6N2E2_2300 0.87 -2.5 2,723,795 - Pf6N2E2_2300 0.87 -2.1 2,724,005 + +0.9 2,724,025 + -1.1 2,724,025 + -0.1 2,724,025 + +0.9 2,724,025 + +0.0 2,724,026 - -0.1 2,724,026 - -1.0 2,724,042 + +0.5 2,724,042 + +0.2 2,724,043 - -1.3 2,724,089 + +1.3 2,724,089 + -0.1 2,724,089 + +1.2 2,724,089 + -1.1 2,724,090 - +0.6 2,724,090 - +0.7 2,724,162 + -0.1 2,724,162 + +1.1 2,724,162 + +1.2 2,724,163 - -0.1 2,724,163 - +2.3 2,724,163 - +0.9 2,724,168 + -1.1 2,724,168 + -0.1 2,724,168 + +1.7 2,724,168 + -0.1 2,724,168 + +0.9 2,724,169 - -0.5 2,724,169 - +1.2 2,724,345 + +0.9 2,724,574 - Pf6N2E2_2301 0.17 +0.6 2,724,663 + Pf6N2E2_2301 0.20 +0.4 2,724,877 + Pf6N2E2_2301 0.28 -0.1
Or see this region's nucleotide sequence