Experiment: With vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1956 and Pf6N2E2_1957 are separated by 3 nucleotides Pf6N2E2_1957 and Pf6N2E2_1958 are separated by 170 nucleotides Pf6N2E2_1958 and Pf6N2E2_1959 are separated by 188 nucleotides
Pf6N2E2_1956 - Outer membrane protein romA, at 2,320,609 to 2,321,745
_1956
Pf6N2E2_1957 - putative inner membrane protein, at 2,321,749 to 2,323,065
_1957
Pf6N2E2_1958 - Predicted dinucleotide-binding enzymes, at 2,323,236 to 2,323,778
_1958
Pf6N2E2_1959 - D-sorbitol dehydrogenase (EC 1.1.1.14) (from data) , at 2,323,967 to 2,324,740
_1959
Position (kb)
2321
2322
2323
2324 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 2320.891 kb on - strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.899 kb on + strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.900 kb on - strand, within Pf6N2E2_1956 at 2320.901 kb on + strand, within Pf6N2E2_1956 at 2320.902 kb on - strand, within Pf6N2E2_1956 at 2320.928 kb on - strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.981 kb on + strand, within Pf6N2E2_1956 at 2320.982 kb on - strand, within Pf6N2E2_1956 at 2321.049 kb on - strand, within Pf6N2E2_1956 at 2321.087 kb on - strand, within Pf6N2E2_1956 at 2321.102 kb on - strand, within Pf6N2E2_1956 at 2321.159 kb on + strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.160 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.276 kb on - strand, within Pf6N2E2_1956 at 2321.419 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.443 kb on + strand, within Pf6N2E2_1956 at 2321.651 kb on - strand at 2321.743 kb on + strand at 2321.743 kb on + strand at 2321.744 kb on - strand at 2321.830 kb on + strand at 2321.830 kb on + strand at 2321.831 kb on - strand at 2321.858 kb on - strand at 2321.858 kb on - strand at 2321.932 kb on - strand, within Pf6N2E2_1957 at 2322.169 kb on - strand, within Pf6N2E2_1957 at 2322.206 kb on + strand, within Pf6N2E2_1957 at 2322.344 kb on + strand, within Pf6N2E2_1957 at 2322.451 kb on + strand, within Pf6N2E2_1957 at 2322.484 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.508 kb on + strand, within Pf6N2E2_1957 at 2322.611 kb on + strand, within Pf6N2E2_1957 at 2322.807 kb on + strand, within Pf6N2E2_1957 at 2322.966 kb on + strand at 2322.966 kb on + strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2322.967 kb on - strand at 2323.029 kb on + strand at 2323.071 kb on - strand at 2323.201 kb on + strand at 2323.201 kb on + strand at 2323.202 kb on - strand at 2323.202 kb on - strand at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.459 kb on + strand, within Pf6N2E2_1958 at 2323.462 kb on - strand, within Pf6N2E2_1958 at 2323.507 kb on - strand, within Pf6N2E2_1958 at 2323.777 kb on - strand at 2323.777 kb on - strand at 2323.800 kb on + strand at 2323.804 kb on + strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.805 kb on - strand at 2323.824 kb on + strand at 2323.999 kb on - strand at 2324.016 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t1 remove 2,320,891 - Pf6N2E2_1956 0.25 +0.5 2,320,899 + Pf6N2E2_1956 0.26 -0.3 2,320,899 + Pf6N2E2_1956 0.26 +1.0 2,320,900 - Pf6N2E2_1956 0.26 -0.9 2,320,900 - Pf6N2E2_1956 0.26 -0.3 2,320,901 + Pf6N2E2_1956 0.26 -0.3 2,320,902 - Pf6N2E2_1956 0.26 -1.3 2,320,928 - Pf6N2E2_1956 0.28 -0.3 2,320,981 + Pf6N2E2_1956 0.33 +0.4 2,320,981 + Pf6N2E2_1956 0.33 -0.1 2,320,982 - Pf6N2E2_1956 0.33 +0.5 2,321,049 - Pf6N2E2_1956 0.39 -0.7 2,321,087 - Pf6N2E2_1956 0.42 +0.4 2,321,102 - Pf6N2E2_1956 0.43 -0.6 2,321,159 + Pf6N2E2_1956 0.48 -0.3 2,321,160 - Pf6N2E2_1956 0.48 +0.9 2,321,160 - Pf6N2E2_1956 0.48 +1.2 2,321,276 - Pf6N2E2_1956 0.59 +2.7 2,321,276 - Pf6N2E2_1956 0.59 -0.9 2,321,419 + Pf6N2E2_1956 0.71 -0.9 2,321,443 + Pf6N2E2_1956 0.73 -0.3 2,321,443 + Pf6N2E2_1956 0.73 -0.6 2,321,443 + Pf6N2E2_1956 0.73 -1.8 2,321,651 - -0.1 2,321,743 + +0.4 2,321,743 + -0.1 2,321,744 - +1.2 2,321,830 + -1.6 2,321,830 + -0.9 2,321,831 - -0.3 2,321,858 - +0.4 2,321,858 - +2.0 2,321,932 - Pf6N2E2_1957 0.14 +0.1 2,322,169 - Pf6N2E2_1957 0.32 -1.3 2,322,206 + Pf6N2E2_1957 0.35 -0.3 2,322,344 + Pf6N2E2_1957 0.45 -1.9 2,322,451 + Pf6N2E2_1957 0.53 -1.3 2,322,484 + Pf6N2E2_1957 0.56 +0.5 2,322,508 + Pf6N2E2_1957 0.58 -2.3 2,322,508 + Pf6N2E2_1957 0.58 -1.3 2,322,611 + Pf6N2E2_1957 0.65 -1.6 2,322,807 + Pf6N2E2_1957 0.80 +0.7 2,322,966 + +0.7 2,322,966 + -0.6 2,322,967 - -0.2 2,322,967 - +0.2 2,322,967 - -0.2 2,323,029 + +0.2 2,323,071 - -2.7 2,323,201 + +0.4 2,323,201 + +1.2 2,323,202 - +0.2 2,323,202 - +1.1 2,323,459 + Pf6N2E2_1958 0.41 -1.0 2,323,459 + Pf6N2E2_1958 0.41 +0.7 2,323,459 + Pf6N2E2_1958 0.41 +1.0 2,323,459 + Pf6N2E2_1958 0.41 -0.6 2,323,462 - Pf6N2E2_1958 0.42 -0.9 2,323,507 - Pf6N2E2_1958 0.50 +0.8 2,323,777 - +0.4 2,323,777 - -1.3 2,323,800 + +0.7 2,323,804 + +0.7 2,323,805 - +0.1 2,323,805 - +1.2 2,323,805 - -0.3 2,323,824 + +0.7 2,323,999 - -0.9 2,324,016 + -0.3
Or see this region's nucleotide sequence