Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1141

Experiment: With vitamins t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1139 and Pf6N2E2_1140 are separated by 33 nucleotidesPf6N2E2_1140 and Pf6N2E2_1141 are separated by 1 nucleotidesPf6N2E2_1141 and Pf6N2E2_1142 are separated by 49 nucleotides Pf6N2E2_1139 - dTDP-glucose 4,6-dehydratase (EC 4.2.1.46), at 1,335,759 to 1,336,730 _1139 Pf6N2E2_1140 - Polymyxin resistance protein ArnC, glycosyl transferase (EC 2.4.-.-), at 1,336,764 to 1,337,507 _1140 Pf6N2E2_1141 - Lipid-A-disaccharide synthase (EC 2.4.1.182), at 1,337,509 to 1,337,802 _1141 Pf6N2E2_1142 - Polymyxin resistance protein ArnT, undecaprenyl phosphate-alpha-L-Ara4N transferase; Melittin resistance protein PqaB, at 1,337,852 to 1,339,327 _1142 Position (kb) 1337 1338Strain fitness (log2 ratio) -2 -1 0 1 2at 1336.707 kb on + strandat 1336.716 kb on + strandat 1336.719 kb on + strandat 1336.720 kb on - strandat 1336.779 kb on + strandat 1336.779 kb on + strandat 1336.780 kb on - strandat 1336.780 kb on - strandat 1336.780 kb on - strandat 1336.783 kb on + strandat 1336.783 kb on + strandat 1336.784 kb on - strandat 1336.817 kb on + strandat 1336.817 kb on + strandat 1336.921 kb on + strand, within Pf6N2E2_1140at 1336.922 kb on - strand, within Pf6N2E2_1140at 1336.922 kb on - strand, within Pf6N2E2_1140at 1337.001 kb on + strand, within Pf6N2E2_1140at 1337.590 kb on + strand, within Pf6N2E2_1141at 1337.600 kb on - strand, within Pf6N2E2_1141at 1337.600 kb on - strand, within Pf6N2E2_1141at 1337.671 kb on + strand, within Pf6N2E2_1141at 1337.671 kb on + strand, within Pf6N2E2_1141at 1337.672 kb on - strand, within Pf6N2E2_1141at 1337.856 kb on + strandat 1337.856 kb on + strandat 1337.856 kb on + strandat 1337.993 kb on + strandat 1338.071 kb on + strand, within Pf6N2E2_1142at 1338.072 kb on - strand, within Pf6N2E2_1142at 1338.072 kb on - strand, within Pf6N2E2_1142at 1338.116 kb on + strand, within Pf6N2E2_1142at 1338.116 kb on + strand, within Pf6N2E2_1142at 1338.194 kb on + strand, within Pf6N2E2_1142at 1338.194 kb on + strand, within Pf6N2E2_1142at 1338.194 kb on + strand, within Pf6N2E2_1142at 1338.257 kb on + strand, within Pf6N2E2_1142at 1338.257 kb on + strand, within Pf6N2E2_1142at 1338.257 kb on + strand, within Pf6N2E2_1142at 1338.258 kb on - strand, within Pf6N2E2_1142at 1338.258 kb on - strand, within Pf6N2E2_1142at 1338.258 kb on - strand, within Pf6N2E2_1142at 1338.302 kb on + strand, within Pf6N2E2_1142at 1338.303 kb on - strand, within Pf6N2E2_1142at 1338.305 kb on + strand, within Pf6N2E2_1142at 1338.305 kb on + strand, within Pf6N2E2_1142at 1338.306 kb on - strand, within Pf6N2E2_1142at 1338.306 kb on - strand, within Pf6N2E2_1142at 1338.369 kb on + strand, within Pf6N2E2_1142at 1338.389 kb on + strand, within Pf6N2E2_1142at 1338.389 kb on + strand, within Pf6N2E2_1142at 1338.542 kb on + strand, within Pf6N2E2_1142at 1338.542 kb on + strand, within Pf6N2E2_1142at 1338.543 kb on - strand, within Pf6N2E2_1142at 1338.543 kb on - strand, within Pf6N2E2_1142at 1338.543 kb on - strand, within Pf6N2E2_1142at 1338.581 kb on + strand, within Pf6N2E2_1142at 1338.617 kb on + strand, within Pf6N2E2_1142at 1338.645 kb on - strand, within Pf6N2E2_1142at 1338.645 kb on - strand, within Pf6N2E2_1142at 1338.794 kb on + strand, within Pf6N2E2_1142at 1338.794 kb on + strand, within Pf6N2E2_1142at 1338.794 kb on + strand, within Pf6N2E2_1142at 1338.794 kb on + strand, within Pf6N2E2_1142at 1338.795 kb on - strand, within Pf6N2E2_1142at 1338.795 kb on - strand, within Pf6N2E2_1142at 1338.795 kb on - strand, within Pf6N2E2_1142at 1338.797 kb on - strand, within Pf6N2E2_1142at 1338.797 kb on - strand, within Pf6N2E2_1142at 1338.797 kb on - strand, within Pf6N2E2_1142

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Per-strain Table

Position Strand Gene LocusTag Fraction With vitamins t1
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1,336,707 + +1.4
1,336,716 + -2.1
1,336,719 + -0.6
1,336,720 - +0.4
1,336,779 + -2.1
1,336,779 + +2.3
1,336,780 - -0.6
1,336,780 - -0.2
1,336,780 - -0.6
1,336,783 + -0.2
1,336,783 + -1.1
1,336,784 - -0.6
1,336,817 + -1.6
1,336,817 + -0.6
1,336,921 + Pf6N2E2_1140 0.21 +0.5
1,336,922 - Pf6N2E2_1140 0.21 +0.4
1,336,922 - Pf6N2E2_1140 0.21 +1.0
1,337,001 + Pf6N2E2_1140 0.32 +0.3
1,337,590 + Pf6N2E2_1141 0.28 -1.6
1,337,600 - Pf6N2E2_1141 0.31 -0.6
1,337,600 - Pf6N2E2_1141 0.31 -0.6
1,337,671 + Pf6N2E2_1141 0.55 -1.6
1,337,671 + Pf6N2E2_1141 0.55 +0.4
1,337,672 - Pf6N2E2_1141 0.55 -1.9
1,337,856 + -0.1
1,337,856 + -0.6
1,337,856 + +0.7
1,337,993 + -1.4
1,338,071 + Pf6N2E2_1142 0.15 -1.6
1,338,072 - Pf6N2E2_1142 0.15 -0.3
1,338,072 - Pf6N2E2_1142 0.15 +2.0
1,338,116 + Pf6N2E2_1142 0.18 -0.6
1,338,116 + Pf6N2E2_1142 0.18 +0.4
1,338,194 + Pf6N2E2_1142 0.23 -0.9
1,338,194 + Pf6N2E2_1142 0.23 +2.4
1,338,194 + Pf6N2E2_1142 0.23 +0.6
1,338,257 + Pf6N2E2_1142 0.27 -0.6
1,338,257 + Pf6N2E2_1142 0.27 +1.0
1,338,257 + Pf6N2E2_1142 0.27 +1.3
1,338,258 - Pf6N2E2_1142 0.28 -0.6
1,338,258 - Pf6N2E2_1142 0.28 +0.4
1,338,258 - Pf6N2E2_1142 0.28 -0.6
1,338,302 + Pf6N2E2_1142 0.30 -0.9
1,338,303 - Pf6N2E2_1142 0.31 -2.6
1,338,305 + Pf6N2E2_1142 0.31 +1.0
1,338,305 + Pf6N2E2_1142 0.31 +1.0
1,338,306 - Pf6N2E2_1142 0.31 -0.6
1,338,306 - Pf6N2E2_1142 0.31 +0.0
1,338,369 + Pf6N2E2_1142 0.35 -0.6
1,338,389 + Pf6N2E2_1142 0.36 +1.0
1,338,389 + Pf6N2E2_1142 0.36 -0.6
1,338,542 + Pf6N2E2_1142 0.47 -0.1
1,338,542 + Pf6N2E2_1142 0.47 +0.4
1,338,543 - Pf6N2E2_1142 0.47 -1.2
1,338,543 - Pf6N2E2_1142 0.47 +0.5
1,338,543 - Pf6N2E2_1142 0.47 -0.6
1,338,581 + Pf6N2E2_1142 0.49 -1.1
1,338,617 + Pf6N2E2_1142 0.52 +0.4
1,338,645 - Pf6N2E2_1142 0.54 -1.7
1,338,645 - Pf6N2E2_1142 0.54 +1.9
1,338,794 + Pf6N2E2_1142 0.64 +0.3
1,338,794 + Pf6N2E2_1142 0.64 +1.6
1,338,794 + Pf6N2E2_1142 0.64 +0.8
1,338,794 + Pf6N2E2_1142 0.64 +0.4
1,338,795 - Pf6N2E2_1142 0.64 -2.6
1,338,795 - Pf6N2E2_1142 0.64 -1.6
1,338,795 - Pf6N2E2_1142 0.64 -0.6
1,338,797 - Pf6N2E2_1142 0.64 +0.2
1,338,797 - Pf6N2E2_1142 0.64 +2.2
1,338,797 - Pf6N2E2_1142 0.64 -0.7

Or see this region's nucleotide sequence