Strain Fitness in Pseudomonas fluorescens FW300-N2E2 around Pf6N2E2_1054

Experiment: With vitamins t1

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPf6N2E2_1053 and Pf6N2E2_1054 are separated by 498 nucleotidesPf6N2E2_1054 and Pf6N2E2_1055 are separated by 153 nucleotides Pf6N2E2_1053 - Bifunctional protein: zinc-containing alcohol dehydrogenase; quinone oxidoreductase ( NADPH:quinone reductase) (EC 1.1.1.-); Similar to arginate lyase, at 1,227,150 to 1,227,593 _1053 Pf6N2E2_1054 - integrase, putative, at 1,228,092 to 1,229,360 _1054 Pf6N2E2_1055 - Transcriptional regulator MexT, at 1,229,514 to 1,230,428 _1055 Position (kb) 1228 1229 1230Strain fitness (log2 ratio) -2 -1 0 1 2 3at 1227.136 kb on + strandat 1227.136 kb on + strandat 1227.137 kb on - strandat 1227.137 kb on - strandat 1227.147 kb on + strandat 1227.147 kb on + strandat 1227.437 kb on - strand, within Pf6N2E2_1053at 1227.519 kb on + strand, within Pf6N2E2_1053at 1227.731 kb on - strandat 1227.772 kb on + strandat 1227.772 kb on + strandat 1227.773 kb on - strandat 1227.796 kb on - strandat 1227.796 kb on - strandat 1227.797 kb on + strandat 1227.798 kb on - strandat 1227.830 kb on + strandat 1227.908 kb on + strandat 1227.935 kb on - strandat 1228.078 kb on + strandat 1228.138 kb on + strandat 1228.231 kb on - strand, within Pf6N2E2_1054at 1228.288 kb on + strand, within Pf6N2E2_1054at 1228.288 kb on + strand, within Pf6N2E2_1054at 1228.289 kb on - strand, within Pf6N2E2_1054at 1228.321 kb on + strand, within Pf6N2E2_1054at 1228.322 kb on - strand, within Pf6N2E2_1054at 1228.335 kb on - strand, within Pf6N2E2_1054at 1228.439 kb on + strand, within Pf6N2E2_1054at 1228.440 kb on - strand, within Pf6N2E2_1054at 1228.440 kb on - strand, within Pf6N2E2_1054at 1228.444 kb on - strand, within Pf6N2E2_1054at 1228.444 kb on - strand, within Pf6N2E2_1054at 1228.811 kb on + strand, within Pf6N2E2_1054at 1228.811 kb on + strand, within Pf6N2E2_1054at 1228.869 kb on - strand, within Pf6N2E2_1054at 1229.011 kb on + strand, within Pf6N2E2_1054at 1229.012 kb on - strand, within Pf6N2E2_1054at 1229.245 kb on - strandat 1229.348 kb on + strandat 1229.621 kb on + strand, within Pf6N2E2_1055at 1229.621 kb on + strand, within Pf6N2E2_1055at 1229.621 kb on + strand, within Pf6N2E2_1055at 1229.622 kb on - strand, within Pf6N2E2_1055at 1229.687 kb on - strand, within Pf6N2E2_1055at 1229.687 kb on - strand, within Pf6N2E2_1055at 1229.804 kb on + strand, within Pf6N2E2_1055at 1229.901 kb on + strand, within Pf6N2E2_1055at 1229.901 kb on + strand, within Pf6N2E2_1055at 1229.902 kb on - strand, within Pf6N2E2_1055at 1229.902 kb on - strand, within Pf6N2E2_1055at 1229.930 kb on + strand, within Pf6N2E2_1055at 1229.930 kb on + strand, within Pf6N2E2_1055at 1229.931 kb on - strand, within Pf6N2E2_1055at 1229.931 kb on - strand, within Pf6N2E2_1055at 1229.963 kb on - strand, within Pf6N2E2_1055at 1229.963 kb on - strand, within Pf6N2E2_1055at 1230.016 kb on + strand, within Pf6N2E2_1055at 1230.016 kb on + strand, within Pf6N2E2_1055at 1230.016 kb on + strand, within Pf6N2E2_1055at 1230.016 kb on + strand, within Pf6N2E2_1055at 1230.017 kb on - strand, within Pf6N2E2_1055at 1230.017 kb on - strand, within Pf6N2E2_1055at 1230.017 kb on - strand, within Pf6N2E2_1055at 1230.254 kb on + strand, within Pf6N2E2_1055at 1230.255 kb on - strand, within Pf6N2E2_1055at 1230.255 kb on - strand, within Pf6N2E2_1055at 1230.255 kb on - strand, within Pf6N2E2_1055at 1230.258 kb on + strand, within Pf6N2E2_1055at 1230.259 kb on - strand, within Pf6N2E2_1055at 1230.259 kb on - strand, within Pf6N2E2_1055at 1230.259 kb on - strand, within Pf6N2E2_1055at 1230.259 kb on - strand, within Pf6N2E2_1055at 1230.259 kb on - strand, within Pf6N2E2_1055

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Per-strain Table

Position Strand Gene LocusTag Fraction With vitamins t1
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1,227,136 + +0.0
1,227,136 + +0.5
1,227,137 - +0.1
1,227,137 - -0.5
1,227,147 + -1.3
1,227,147 + -0.5
1,227,437 - Pf6N2E2_1053 0.65 -0.5
1,227,519 + Pf6N2E2_1053 0.83 -2.1
1,227,731 - -1.1
1,227,772 + -1.9
1,227,772 + -0.5
1,227,773 - -1.1
1,227,796 - +1.6
1,227,796 - -1.5
1,227,797 + -2.5
1,227,798 - -0.5
1,227,830 + -0.5
1,227,908 + -0.2
1,227,935 - +0.5
1,228,078 + -1.1
1,228,138 + +0.8
1,228,231 - Pf6N2E2_1054 0.11 -2.0
1,228,288 + Pf6N2E2_1054 0.15 -0.7
1,228,288 + Pf6N2E2_1054 0.15 -1.5
1,228,289 - Pf6N2E2_1054 0.16 -1.0
1,228,321 + Pf6N2E2_1054 0.18 +0.8
1,228,322 - Pf6N2E2_1054 0.18 -2.1
1,228,335 - Pf6N2E2_1054 0.19 +2.8
1,228,439 + Pf6N2E2_1054 0.27 -1.5
1,228,440 - Pf6N2E2_1054 0.27 -0.5
1,228,440 - Pf6N2E2_1054 0.27 -1.5
1,228,444 - Pf6N2E2_1054 0.28 -1.5
1,228,444 - Pf6N2E2_1054 0.28 -0.5
1,228,811 + Pf6N2E2_1054 0.57 -0.7
1,228,811 + Pf6N2E2_1054 0.57 -0.1
1,228,869 - Pf6N2E2_1054 0.61 -1.9
1,229,011 + Pf6N2E2_1054 0.72 -0.3
1,229,012 - Pf6N2E2_1054 0.72 -0.5
1,229,245 - +0.6
1,229,348 + +0.0
1,229,621 + Pf6N2E2_1055 0.12 +0.2
1,229,621 + Pf6N2E2_1055 0.12 -1.5
1,229,621 + Pf6N2E2_1055 0.12 -0.3
1,229,622 - Pf6N2E2_1055 0.12 +1.0
1,229,687 - Pf6N2E2_1055 0.19 +0.2
1,229,687 - Pf6N2E2_1055 0.19 +0.1
1,229,804 + Pf6N2E2_1055 0.32 -1.0
1,229,901 + Pf6N2E2_1055 0.42 +0.6
1,229,901 + Pf6N2E2_1055 0.42 -1.5
1,229,902 - Pf6N2E2_1055 0.42 +0.5
1,229,902 - Pf6N2E2_1055 0.42 +1.0
1,229,930 + Pf6N2E2_1055 0.45 +0.1
1,229,930 + Pf6N2E2_1055 0.45 -0.4
1,229,931 - Pf6N2E2_1055 0.46 -0.1
1,229,931 - Pf6N2E2_1055 0.46 -2.0
1,229,963 - Pf6N2E2_1055 0.49 -0.1
1,229,963 - Pf6N2E2_1055 0.49 -0.3
1,230,016 + Pf6N2E2_1055 0.55 +0.1
1,230,016 + Pf6N2E2_1055 0.55 -0.5
1,230,016 + Pf6N2E2_1055 0.55 +0.0
1,230,016 + Pf6N2E2_1055 0.55 +2.6
1,230,017 - Pf6N2E2_1055 0.55 +1.0
1,230,017 - Pf6N2E2_1055 0.55 -2.1
1,230,017 - Pf6N2E2_1055 0.55 +0.9
1,230,254 + Pf6N2E2_1055 0.81 -0.5
1,230,255 - Pf6N2E2_1055 0.81 +0.0
1,230,255 - Pf6N2E2_1055 0.81 +0.3
1,230,255 - Pf6N2E2_1055 0.81 -0.5
1,230,258 + Pf6N2E2_1055 0.81 +1.0
1,230,259 - Pf6N2E2_1055 0.81 +0.5
1,230,259 - Pf6N2E2_1055 0.81 +0.5
1,230,259 - Pf6N2E2_1055 0.81 +1.0
1,230,259 - Pf6N2E2_1055 0.81 +0.7
1,230,259 - Pf6N2E2_1055 0.81 +0.5

Or see this region's nucleotide sequence