Experiment: With vitamins t1
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Pf6N2E2_1023 and Pf6N2E2_1024 overlap by 4 nucleotides Pf6N2E2_1024 and Pf6N2E2_1025 are separated by 12 nucleotides
Pf6N2E2_1023 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,196,073 to 1,197,065
_1023
Pf6N2E2_1024 - Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases, at 1,197,062 to 1,198,414
_1024
Pf6N2E2_1025 - ABC-type nitrate/sulfonate/bicarbonate transport systems, periplasmic components, at 1,198,427 to 1,199,380
_1025
Position (kb)
1197
1198
1199 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.233 kb on + strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.234 kb on - strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.344 kb on + strand, within Pf6N2E2_1023 at 1196.407 kb on + strand, within Pf6N2E2_1023 at 1196.407 kb on + strand, within Pf6N2E2_1023 at 1196.408 kb on - strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.488 kb on + strand, within Pf6N2E2_1023 at 1196.489 kb on - strand, within Pf6N2E2_1023 at 1196.869 kb on + strand, within Pf6N2E2_1023 at 1196.923 kb on + strand, within Pf6N2E2_1023 at 1197.057 kb on + strand at 1197.058 kb on - strand at 1197.058 kb on - strand at 1197.154 kb on + strand at 1197.155 kb on - strand at 1197.675 kb on + strand, within Pf6N2E2_1024 at 1197.897 kb on + strand, within Pf6N2E2_1024 at 1197.898 kb on - strand, within Pf6N2E2_1024 at 1198.116 kb on + strand, within Pf6N2E2_1024 at 1198.207 kb on - strand, within Pf6N2E2_1024 at 1198.299 kb on + strand at 1198.527 kb on + strand, within Pf6N2E2_1025 at 1198.528 kb on - strand, within Pf6N2E2_1025 at 1198.528 kb on - strand, within Pf6N2E2_1025 at 1198.736 kb on - strand, within Pf6N2E2_1025 at 1198.767 kb on + strand, within Pf6N2E2_1025 at 1198.767 kb on + strand, within Pf6N2E2_1025 at 1198.974 kb on + strand, within Pf6N2E2_1025 at 1199.071 kb on + strand, within Pf6N2E2_1025 at 1199.072 kb on - strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.139 kb on + strand, within Pf6N2E2_1025 at 1199.140 kb on - strand, within Pf6N2E2_1025 at 1199.140 kb on - strand, within Pf6N2E2_1025
Per-strain Table
Position Strand Gene LocusTag Fraction With vitamins t1 remove 1,196,233 + Pf6N2E2_1023 0.16 -1.8 1,196,233 + Pf6N2E2_1023 0.16 -0.4 1,196,233 + Pf6N2E2_1023 0.16 -2.1 1,196,233 + Pf6N2E2_1023 0.16 +0.7 1,196,233 + Pf6N2E2_1023 0.16 -0.5 1,196,233 + Pf6N2E2_1023 0.16 -2.1 1,196,233 + Pf6N2E2_1023 0.16 -0.2 1,196,234 - Pf6N2E2_1023 0.16 -0.5 1,196,234 - Pf6N2E2_1023 0.16 -0.5 1,196,234 - Pf6N2E2_1023 0.16 -0.5 1,196,234 - Pf6N2E2_1023 0.16 -0.5 1,196,344 + Pf6N2E2_1023 0.27 -0.3 1,196,344 + Pf6N2E2_1023 0.27 +2.5 1,196,344 + Pf6N2E2_1023 0.27 +0.1 1,196,407 + Pf6N2E2_1023 0.34 +0.5 1,196,407 + Pf6N2E2_1023 0.34 -0.1 1,196,408 - Pf6N2E2_1023 0.34 -0.5 1,196,488 + Pf6N2E2_1023 0.42 +1.1 1,196,488 + Pf6N2E2_1023 0.42 +1.1 1,196,488 + Pf6N2E2_1023 0.42 -0.7 1,196,489 - Pf6N2E2_1023 0.42 +1.1 1,196,869 + Pf6N2E2_1023 0.80 -1.1 1,196,923 + Pf6N2E2_1023 0.86 +1.1 1,197,057 + -0.9 1,197,058 - -0.5 1,197,058 - -1.1 1,197,154 + -0.5 1,197,155 - +1.3 1,197,675 + Pf6N2E2_1024 0.45 -1.5 1,197,897 + Pf6N2E2_1024 0.62 -1.5 1,197,898 - Pf6N2E2_1024 0.62 -0.5 1,198,116 + Pf6N2E2_1024 0.78 -0.9 1,198,207 - Pf6N2E2_1024 0.85 +0.2 1,198,299 + +1.5 1,198,527 + Pf6N2E2_1025 0.10 -0.9 1,198,528 - Pf6N2E2_1025 0.11 +0.2 1,198,528 - Pf6N2E2_1025 0.11 -3.3 1,198,736 - Pf6N2E2_1025 0.32 -0.5 1,198,767 + Pf6N2E2_1025 0.36 -3.1 1,198,767 + Pf6N2E2_1025 0.36 +0.2 1,198,974 + Pf6N2E2_1025 0.57 -1.0 1,199,071 + Pf6N2E2_1025 0.68 +0.5 1,199,072 - Pf6N2E2_1025 0.68 +0.1 1,199,139 + Pf6N2E2_1025 0.75 +1.1 1,199,139 + Pf6N2E2_1025 0.75 -0.5 1,199,139 + Pf6N2E2_1025 0.75 +1.1 1,199,139 + Pf6N2E2_1025 0.75 -1.2 1,199,140 - Pf6N2E2_1025 0.75 -0.1 1,199,140 - Pf6N2E2_1025 0.75 -0.5
Or see this region's nucleotide sequence