Strain Fitness in Pseudomonas fluorescens SBW25-INTG around PFLU_RS25595

Experiment: malate (C) and Ammonium chloride (N); with MOPS; with PEG

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntPFLU_RS25590 and PFLU_RS25595 overlap by 4 nucleotidesPFLU_RS25595 and PFLU_RS25600 are separated by 144 nucleotidesPFLU_RS25600 and PFLU_RS25605 are separated by 140 nucleotides PFLU_RS25590: PFLU_RS25590 - exodeoxyribonuclease V subunit beta, at 5,709,780 to 5,713,454 _RS25590 PFLU_RS25595: PFLU_RS25595 - exodeoxyribonuclease V subunit gamma, at 5,713,451 to 5,716,900 _RS25595 PFLU_RS25600: PFLU_RS25600 - 5S ribosomal RNA, at 5,717,045 to 5,717,160 _RS25600 PFLU_RS25605: PFLU_RS25605 - 23S ribosomal RNA, at 5,717,301 to 5,720,216 _RS25605 Position (kb) 5713 5714 5715 5716 5717Strain fitness (log2 ratio) -2 -1 0 1 2 3at 5712.852 kb on + strand, within PFLU_RS25590at 5712.853 kb on - strand, within PFLU_RS25590at 5713.446 kb on - strandat 5714.058 kb on - strand, within PFLU_RS25595at 5714.310 kb on - strand, within PFLU_RS25595at 5715.194 kb on + strand, within PFLU_RS25595at 5715.195 kb on - strand, within PFLU_RS25595at 5715.195 kb on - strand, within PFLU_RS25595at 5715.912 kb on + strand, within PFLU_RS25595at 5715.912 kb on + strand, within PFLU_RS25595at 5716.042 kb on - strand, within PFLU_RS25595at 5716.295 kb on + strand, within PFLU_RS25595at 5716.296 kb on - strand, within PFLU_RS25595at 5716.296 kb on - strand, within PFLU_RS25595at 5716.901 kb on - strandat 5716.943 kb on - strandat 5716.958 kb on + strandat 5716.958 kb on + strandat 5716.958 kb on + strandat 5716.958 kb on + strandat 5716.959 kb on - strandat 5716.959 kb on - strandat 5716.959 kb on - strandat 5716.980 kb on + strandat 5716.980 kb on + strandat 5716.980 kb on + strandat 5716.980 kb on + strandat 5716.981 kb on - strandat 5716.981 kb on - strandat 5716.981 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction malate (C) and Ammonium chloride (N); with MOPS; with PEG
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5,712,852 + PFLU_RS25590 0.84 +0.2
5,712,853 - PFLU_RS25590 0.84 -0.2
5,713,446 - -0.5
5,714,058 - PFLU_RS25595 0.18 -0.6
5,714,310 - PFLU_RS25595 0.25 -1.0
5,715,194 + PFLU_RS25595 0.51 -1.4
5,715,195 - PFLU_RS25595 0.51 -2.5
5,715,195 - PFLU_RS25595 0.51 -1.8
5,715,912 + PFLU_RS25595 0.71 -1.0
5,715,912 + PFLU_RS25595 0.71 -0.6
5,716,042 - PFLU_RS25595 0.75 +0.1
5,716,295 + PFLU_RS25595 0.82 -1.8
5,716,296 - PFLU_RS25595 0.82 -0.8
5,716,296 - PFLU_RS25595 0.82 -1.0
5,716,901 - +0.0
5,716,943 - -0.8
5,716,958 + -1.8
5,716,958 + -0.3
5,716,958 + -0.1
5,716,958 + -0.3
5,716,959 - -0.6
5,716,959 - +0.7
5,716,959 - +0.3
5,716,980 + +1.6
5,716,980 + +1.8
5,716,980 + -0.6
5,716,980 + +1.5
5,716,981 - -0.2
5,716,981 - -0.4
5,716,981 - +2.8

Or see this region's nucleotide sequence