Experiment: MoLS4 without B12 vitamin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt trpC and trpF-1 are separated by 47 nucleotides trpF-1 and trpB-2 overlap by 4 nucleotides trpB-2 and trpA overlap by 4 nucleotides
DVU0468: trpC - indole-3-glycerol phosphate synthase (TIGR), at 536,049 to 536,822
trpC
DVU0469: trpF-1 - N-(5-phosphoribosyl)anthranilate isomerase (TIGR), at 536,870 to 537,724
trpF-1
DVU0470: trpB-2 - tryptophan synthase, beta subunit (TIGR), at 537,721 to 538,908
trpB-2
DVU0471: trpA - tryptophan synthase, alpha subunit (TIGR), at 538,905 to 539,678
trpA
Position (kb) 537
538
539 Strain fitness (log2 ratio) -6
-5
-4
-3
-2
-1
0
1
2 at 536.750 kb on - strand at 536.757 kb on + strand at 536.757 kb on + strand at 536.765 kb on - strand at 536.799 kb on + strand at 536.807 kb on - strand at 536.860 kb on + strand at 536.979 kb on + strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.037 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.044 kb on - strand, within trpF-1 at 537.135 kb on + strand, within trpF-1 at 537.206 kb on - strand, within trpF-1 at 537.288 kb on + strand, within trpF-1 at 537.389 kb on - strand, within trpF-1 at 537.476 kb on + strand, within trpF-1 at 537.479 kb on - strand, within trpF-1 at 537.489 kb on - strand, within trpF-1 at 537.574 kb on - strand, within trpF-1 at 537.579 kb on - strand, within trpF-1 at 537.664 kb on - strand at 537.671 kb on - strand at 537.688 kb on - strand at 537.690 kb on + strand at 537.692 kb on + strand at 538.023 kb on - strand, within trpB-2 at 538.233 kb on + strand, within trpB-2 at 538.236 kb on - strand, within trpB-2 at 538.244 kb on - strand, within trpB-2 at 538.254 kb on + strand, within trpB-2 at 538.264 kb on + strand, within trpB-2 at 538.432 kb on + strand, within trpB-2 at 538.527 kb on - strand, within trpB-2 at 538.529 kb on - strand, within trpB-2 at 538.594 kb on - strand, within trpB-2 at 538.814 kb on + strand at 538.825 kb on - strand at 538.855 kb on + strand at 538.863 kb on - strand at 538.863 kb on - strand at 538.873 kb on + strand at 539.142 kb on - strand, within trpA at 539.152 kb on - strand, within trpA at 539.178 kb on + strand, within trpA at 539.194 kb on + strand, within trpA at 539.202 kb on - strand, within trpA at 539.202 kb on - strand, within trpA at 539.202 kb on - strand, within trpA at 539.213 kb on + strand, within trpA at 539.279 kb on - strand, within trpA at 539.366 kb on + strand, within trpA at 539.371 kb on + strand, within trpA at 539.434 kb on - strand, within trpA at 539.450 kb on - strand, within trpA at 539.618 kb on - strand at 539.812 kb on + strand at 539.872 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin remove 536,750 - -1.3 536,757 + -2.6 536,757 + -0.4 536,765 - -4.3 536,799 + -1.3 536,807 - -2.8 536,860 + -2.4 536,979 + trpF-1 DVU0469 0.13 -3.2 537,037 - trpF-1 DVU0469 0.20 -0.5 537,037 - trpF-1 DVU0469 0.20 -3.3 537,044 - trpF-1 DVU0469 0.20 -3.1 537,044 - trpF-1 DVU0469 0.20 -3.8 537,135 + trpF-1 DVU0469 0.31 -1.8 537,206 - trpF-1 DVU0469 0.39 +0.8 537,288 + trpF-1 DVU0469 0.49 -3.4 537,389 - trpF-1 DVU0469 0.61 -5.2 537,476 + trpF-1 DVU0469 0.71 -0.6 537,479 - trpF-1 DVU0469 0.71 -2.9 537,489 - trpF-1 DVU0469 0.72 +2.4 537,574 - trpF-1 DVU0469 0.82 -3.3 537,579 - trpF-1 DVU0469 0.83 -0.6 537,664 - -0.6 537,671 - -0.8 537,688 - -0.0 537,690 + +1.1 537,692 + -0.5 538,023 - trpB-2 DVU0470 0.25 +0.4 538,233 + trpB-2 DVU0470 0.43 -2.7 538,236 - trpB-2 DVU0470 0.43 -0.4 538,244 - trpB-2 DVU0470 0.44 -3.1 538,254 + trpB-2 DVU0470 0.45 -1.2 538,264 + trpB-2 DVU0470 0.46 -2.2 538,432 + trpB-2 DVU0470 0.60 -0.2 538,527 - trpB-2 DVU0470 0.68 +0.8 538,529 - trpB-2 DVU0470 0.68 -4.2 538,594 - trpB-2 DVU0470 0.73 -4.5 538,814 + -1.7 538,825 - -0.9 538,855 + +0.4 538,863 - -0.6 538,863 - -1.8 538,873 + -0.5 539,142 - trpA DVU0471 0.31 -4.1 539,152 - trpA DVU0471 0.32 -2.8 539,178 + trpA DVU0471 0.35 -4.1 539,194 + trpA DVU0471 0.37 -1.9 539,202 - trpA DVU0471 0.38 -4.1 539,202 - trpA DVU0471 0.38 -6.1 539,202 - trpA DVU0471 0.38 -0.2 539,213 + trpA DVU0471 0.40 -2.6 539,279 - trpA DVU0471 0.48 -5.3 539,366 + trpA DVU0471 0.60 -2.4 539,371 + trpA DVU0471 0.60 +0.1 539,434 - trpA DVU0471 0.68 -2.0 539,450 - trpA DVU0471 0.70 -0.2 539,618 - -0.9 539,812 + -0.9 539,872 - +2.2
Or see this region's nucleotide sequence