Experiment: MoLS4 without B12 vitamin
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt slt and DVU2700 overlap by 8 nucleotides DVU2700 and DVU2701 are separated by 28 nucleotides DVU2701 and DVU2702 are separated by 3 nucleotides DVU2702 and DVU2703 overlap by 14 nucleotides DVU2703 and DVU2704 are separated by 1 nucleotides DVU2704 and DVU2705 overlap by 4 nucleotides
DVU2699: slt - transglycosylase SLT domain protein (TIGR), at 2,808,534 to 2,809,181
slt
DVU2700: DVU2700 - hypothetical protein (TIGR), at 2,809,174 to 2,809,593
DVU2700
DVU2701: DVU2701 - hypothetical protein (TIGR), at 2,809,622 to 2,809,840
DVU2701
DVU2702: DVU2702 - conserved hypothetical protein (TIGR), at 2,809,844 to 2,810,125
DVU2702
DVU2703: DVU2703 - hypothetical protein (TIGR), at 2,810,112 to 2,810,327
DVU2703
DVU2704: DVU2704 - conserved hypothetical protein (TIGR), at 2,810,329 to 2,810,847
DVU2704
DVU2705: DVU2705 - phage uncharacterized protein (TIGR), at 2,810,844 to 2,812,520
DVU2705
Position (kb)
2810
2811 Strain fitness (log2 ratio)
-1
0
1
2
3 at 2809.208 kb on + strand at 2809.234 kb on - strand, within DVU2700 at 2809.318 kb on + strand, within DVU2700 at 2809.320 kb on + strand, within DVU2700 at 2809.326 kb on - strand, within DVU2700 at 2809.326 kb on - strand, within DVU2700 at 2809.333 kb on - strand, within DVU2700 at 2809.340 kb on - strand, within DVU2700 at 2809.344 kb on + strand, within DVU2700 at 2809.344 kb on + strand, within DVU2700 at 2809.344 kb on + strand, within DVU2700 at 2809.352 kb on - strand, within DVU2700 at 2809.392 kb on + strand, within DVU2700 at 2809.406 kb on - strand, within DVU2700 at 2809.489 kb on - strand, within DVU2700 at 2809.505 kb on - strand, within DVU2700 at 2809.637 kb on + strand at 2809.637 kb on + strand at 2809.832 kb on - strand at 2809.874 kb on - strand, within DVU2702 at 2809.904 kb on + strand, within DVU2702 at 2809.937 kb on + strand, within DVU2702 at 2809.947 kb on + strand, within DVU2702 at 2809.985 kb on - strand, within DVU2702 at 2809.991 kb on - strand, within DVU2702 at 2810.021 kb on - strand, within DVU2702 at 2810.038 kb on + strand, within DVU2702 at 2810.067 kb on - strand, within DVU2702 at 2810.084 kb on + strand, within DVU2702 at 2810.092 kb on - strand, within DVU2702 at 2810.212 kb on + strand, within DVU2703 at 2810.303 kb on + strand, within DVU2703 at 2810.375 kb on + strand at 2810.383 kb on - strand, within DVU2704 at 2810.505 kb on + strand, within DVU2704 at 2810.562 kb on + strand, within DVU2704 at 2810.570 kb on - strand, within DVU2704 at 2810.576 kb on + strand, within DVU2704 at 2810.681 kb on + strand, within DVU2704 at 2810.720 kb on + strand, within DVU2704 at 2810.787 kb on + strand, within DVU2704 at 2810.787 kb on + strand, within DVU2704 at 2810.795 kb on - strand, within DVU2704 at 2810.804 kb on - strand at 2810.811 kb on - strand at 2810.843 kb on + strand at 2810.900 kb on + strand at 2810.915 kb on - strand at 2811.024 kb on - strand, within DVU2705 at 2811.069 kb on + strand, within DVU2705 at 2811.069 kb on + strand, within DVU2705 at 2811.077 kb on - strand, within DVU2705 at 2811.077 kb on - strand, within DVU2705 at 2811.110 kb on + strand, within DVU2705 at 2811.197 kb on - strand, within DVU2705 at 2811.252 kb on - strand, within DVU2705 at 2811.252 kb on - strand, within DVU2705 at 2811.309 kb on + strand, within DVU2705
Per-strain Table
Position Strand Gene LocusTag Fraction MoLS4 without B12 vitamin remove 2,809,208 + +0.4 2,809,234 - DVU2700 0.14 +0.5 2,809,318 + DVU2700 0.34 -0.4 2,809,320 + DVU2700 0.35 +1.4 2,809,326 - DVU2700 0.36 -0.7 2,809,326 - DVU2700 0.36 -0.1 2,809,333 - DVU2700 0.38 +0.2 2,809,340 - DVU2700 0.40 +0.1 2,809,344 + DVU2700 0.40 -0.5 2,809,344 + DVU2700 0.40 -0.8 2,809,344 + DVU2700 0.40 +0.2 2,809,352 - DVU2700 0.42 +0.0 2,809,392 + DVU2700 0.52 -0.6 2,809,406 - DVU2700 0.55 +1.8 2,809,489 - DVU2700 0.75 +0.3 2,809,505 - DVU2700 0.79 +0.6 2,809,637 + -0.1 2,809,637 + -0.3 2,809,832 - -0.2 2,809,874 - DVU2702 0.11 -0.9 2,809,904 + DVU2702 0.21 -0.6 2,809,937 + DVU2702 0.33 +1.4 2,809,947 + DVU2702 0.37 +1.0 2,809,985 - DVU2702 0.50 -0.6 2,809,991 - DVU2702 0.52 -0.2 2,810,021 - DVU2702 0.63 +0.1 2,810,038 + DVU2702 0.69 -0.3 2,810,067 - DVU2702 0.79 -0.3 2,810,084 + DVU2702 0.85 +0.1 2,810,092 - DVU2702 0.88 -1.0 2,810,212 + DVU2703 0.46 -1.6 2,810,303 + DVU2703 0.88 -0.2 2,810,375 + -0.1 2,810,383 - DVU2704 0.10 +0.0 2,810,505 + DVU2704 0.34 -0.1 2,810,562 + DVU2704 0.45 +0.5 2,810,570 - DVU2704 0.46 -0.4 2,810,576 + DVU2704 0.48 -0.1 2,810,681 + DVU2704 0.68 -0.3 2,810,720 + DVU2704 0.75 +3.4 2,810,787 + DVU2704 0.88 -0.5 2,810,787 + DVU2704 0.88 +0.8 2,810,795 - DVU2704 0.90 -0.4 2,810,804 - -0.4 2,810,811 - +0.8 2,810,843 + -0.7 2,810,900 + -0.8 2,810,915 - -0.8 2,811,024 - DVU2705 0.11 -0.4 2,811,069 + DVU2705 0.13 -0.2 2,811,069 + DVU2705 0.13 -0.3 2,811,077 - DVU2705 0.14 +0.2 2,811,077 - DVU2705 0.14 -0.9 2,811,110 + DVU2705 0.16 +1.4 2,811,197 - DVU2705 0.21 +0.1 2,811,252 - DVU2705 0.24 -0.5 2,811,252 - DVU2705 0.24 +1.4 2,811,309 + DVU2705 0.28 +2.4
Or see this region's nucleotide sequence