Experiment: Carbon source L-Citrulline 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11730 and PGA1_c11740 are separated by 40 nucleotides PGA1_c11740 and PGA1_c11750 are separated by 86 nucleotides PGA1_c11750 and PGA1_c11760 are separated by 135 nucleotides PGA1_c11760 and PGA1_c11770 are separated by 69 nucleotides
PGA1_c11730: PGA1_c11730 - putative glutathione-dependent formaldehyde-activating enzyme, at 1,214,668 to 1,215,084
_c11730
PGA1_c11740: PGA1_c11740 - rhomboid protein-like protein, at 1,215,125 to 1,215,901
_c11740
PGA1_c11750: PGA1_c11750 - L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (from data) , at 1,215,988 to 1,219,464
_c11750
PGA1_c11760: PGA1_c11760 - putative proline dehydrogenase transcriptional activator, at 1,219,600 to 1,220,073
_c11760
PGA1_c11770: PGA1_c11770 - tetratricopeptide repeat-containing protein, at 1,220,143 to 1,220,775
_c11770
Position (kb)
1215
1216
1217
1218
1219
1220 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1 at 1215.041 kb on - strand, within PGA1_c11730 at 1215.041 kb on - strand, within PGA1_c11730 at 1215.063 kb on + strand at 1215.071 kb on - strand at 1215.072 kb on + strand at 1215.073 kb on + strand at 1215.090 kb on - strand at 1215.106 kb on + strand at 1215.106 kb on + strand at 1215.107 kb on + strand at 1215.114 kb on - strand at 1215.114 kb on - strand at 1215.124 kb on - strand at 1215.192 kb on - strand at 1215.192 kb on - strand at 1215.208 kb on + strand, within PGA1_c11740 at 1215.250 kb on - strand, within PGA1_c11740 at 1215.290 kb on - strand, within PGA1_c11740 at 1215.346 kb on + strand, within PGA1_c11740 at 1215.346 kb on + strand, within PGA1_c11740 at 1215.376 kb on + strand, within PGA1_c11740 at 1215.400 kb on - strand, within PGA1_c11740 at 1215.409 kb on + strand, within PGA1_c11740 at 1215.542 kb on - strand, within PGA1_c11740 at 1215.578 kb on + strand, within PGA1_c11740 at 1215.578 kb on + strand, within PGA1_c11740 at 1215.745 kb on - strand, within PGA1_c11740 at 1215.752 kb on - strand, within PGA1_c11740 at 1215.763 kb on - strand, within PGA1_c11740 at 1215.790 kb on + strand, within PGA1_c11740 at 1215.794 kb on + strand, within PGA1_c11740 at 1215.794 kb on + strand, within PGA1_c11740 at 1215.844 kb on + strand at 1215.844 kb on + strand at 1215.847 kb on - strand at 1215.852 kb on - strand at 1215.852 kb on - strand at 1215.856 kb on + strand at 1215.908 kb on + strand at 1215.920 kb on + strand at 1215.950 kb on + strand at 1216.129 kb on + strand at 1216.257 kb on - strand at 1216.264 kb on + strand at 1216.411 kb on + strand, within PGA1_c11750 at 1216.489 kb on - strand, within PGA1_c11750 at 1216.578 kb on + strand, within PGA1_c11750 at 1216.585 kb on - strand, within PGA1_c11750 at 1216.612 kb on + strand, within PGA1_c11750 at 1216.644 kb on + strand, within PGA1_c11750 at 1216.656 kb on + strand, within PGA1_c11750 at 1216.656 kb on + strand, within PGA1_c11750 at 1216.659 kb on - strand, within PGA1_c11750 at 1216.664 kb on - strand, within PGA1_c11750 at 1216.664 kb on - strand, within PGA1_c11750 at 1216.690 kb on - strand, within PGA1_c11750 at 1216.692 kb on + strand, within PGA1_c11750 at 1216.736 kb on + strand, within PGA1_c11750 at 1216.853 kb on + strand, within PGA1_c11750 at 1216.861 kb on - strand, within PGA1_c11750 at 1216.913 kb on + strand, within PGA1_c11750 at 1216.921 kb on + strand, within PGA1_c11750 at 1216.939 kb on + strand, within PGA1_c11750 at 1216.958 kb on + strand, within PGA1_c11750 at 1217.016 kb on + strand, within PGA1_c11750 at 1217.016 kb on + strand, within PGA1_c11750 at 1217.016 kb on + strand, within PGA1_c11750 at 1217.026 kb on + strand, within PGA1_c11750 at 1217.029 kb on - strand, within PGA1_c11750 at 1217.029 kb on - strand, within PGA1_c11750 at 1217.029 kb on - strand, within PGA1_c11750 at 1217.041 kb on - strand, within PGA1_c11750 at 1217.229 kb on + strand, within PGA1_c11750 at 1217.231 kb on - strand, within PGA1_c11750 at 1217.500 kb on + strand, within PGA1_c11750 at 1217.611 kb on + strand, within PGA1_c11750 at 1217.652 kb on + strand, within PGA1_c11750 at 1217.662 kb on + strand, within PGA1_c11750 at 1217.715 kb on - strand, within PGA1_c11750 at 1217.899 kb on - strand, within PGA1_c11750 at 1217.919 kb on + strand, within PGA1_c11750 at 1217.957 kb on + strand, within PGA1_c11750 at 1218.123 kb on + strand, within PGA1_c11750 at 1218.210 kb on + strand, within PGA1_c11750 at 1218.338 kb on + strand, within PGA1_c11750 at 1218.338 kb on + strand, within PGA1_c11750 at 1218.338 kb on + strand, within PGA1_c11750 at 1218.346 kb on - strand, within PGA1_c11750 at 1218.346 kb on - strand, within PGA1_c11750 at 1218.361 kb on - strand, within PGA1_c11750 at 1218.361 kb on - strand, within PGA1_c11750 at 1218.462 kb on + strand, within PGA1_c11750 at 1218.469 kb on + strand, within PGA1_c11750 at 1218.470 kb on - strand, within PGA1_c11750 at 1218.493 kb on + strand, within PGA1_c11750 at 1218.537 kb on + strand, within PGA1_c11750 at 1218.669 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.736 kb on + strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.945 kb on - strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.985 kb on + strand, within PGA1_c11750 at 1218.990 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.055 kb on + strand, within PGA1_c11750 at 1219.063 kb on - strand, within PGA1_c11750 at 1219.073 kb on - strand, within PGA1_c11750 at 1219.076 kb on + strand, within PGA1_c11750 at 1219.175 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.180 kb on + strand at 1219.239 kb on + strand at 1219.395 kb on + strand at 1219.402 kb on + strand at 1219.651 kb on + strand, within PGA1_c11760 at 1219.651 kb on + strand, within PGA1_c11760 at 1219.665 kb on - strand, within PGA1_c11760 at 1219.687 kb on - strand, within PGA1_c11760 at 1219.748 kb on - strand, within PGA1_c11760 at 1219.803 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.897 kb on + strand, within PGA1_c11760 at 1219.989 kb on + strand, within PGA1_c11760 at 1220.017 kb on + strand, within PGA1_c11760 at 1220.025 kb on - strand, within PGA1_c11760 at 1220.060 kb on - strand at 1220.070 kb on - strand at 1220.133 kb on + strand at 1220.133 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.151 kb on + strand at 1220.159 kb on - strand at 1220.159 kb on - strand at 1220.210 kb on - strand, within PGA1_c11770 at 1220.224 kb on - strand, within PGA1_c11770 at 1220.230 kb on + strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.290 kb on - strand, within PGA1_c11770 at 1220.305 kb on + strand, within PGA1_c11770
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source L-Citrulline 5 mM remove 1,215,041 - PGA1_c11730 0.89 +1.2 1,215,041 - PGA1_c11730 0.89 +0.2 1,215,063 + +0.1 1,215,071 - +0.6 1,215,072 + +0.2 1,215,073 + -0.1 1,215,090 - -0.7 1,215,106 + +0.9 1,215,106 + +0.4 1,215,107 + -0.3 1,215,114 - -0.4 1,215,114 - -1.3 1,215,124 - +0.5 1,215,192 - -1.2 1,215,192 - -0.1 1,215,208 + PGA1_c11740 0.11 +0.6 1,215,250 - PGA1_c11740 0.16 -0.1 1,215,290 - PGA1_c11740 0.21 -0.9 1,215,346 + PGA1_c11740 0.28 -1.5 1,215,346 + PGA1_c11740 0.28 -0.5 1,215,376 + PGA1_c11740 0.32 -1.0 1,215,400 - PGA1_c11740 0.35 +0.3 1,215,409 + PGA1_c11740 0.37 +0.7 1,215,542 - PGA1_c11740 0.54 -0.7 1,215,578 + PGA1_c11740 0.58 +0.4 1,215,578 + PGA1_c11740 0.58 -0.5 1,215,745 - PGA1_c11740 0.80 -0.5 1,215,752 - PGA1_c11740 0.81 -0.1 1,215,763 - PGA1_c11740 0.82 +0.2 1,215,790 + PGA1_c11740 0.86 +0.5 1,215,794 + PGA1_c11740 0.86 -1.2 1,215,794 + PGA1_c11740 0.86 -0.1 1,215,844 + -0.1 1,215,844 + +0.3 1,215,847 - -1.9 1,215,852 - -0.5 1,215,852 - -0.0 1,215,856 + +0.4 1,215,908 + -0.2 1,215,920 + -0.2 1,215,950 + +1.7 1,216,129 + -2.2 1,216,257 - -1.1 1,216,264 + -3.2 1,216,411 + PGA1_c11750 0.12 -2.2 1,216,489 - PGA1_c11750 0.14 -1.7 1,216,578 + PGA1_c11750 0.17 -3.3 1,216,585 - PGA1_c11750 0.17 -1.7 1,216,612 + PGA1_c11750 0.18 -1.3 1,216,644 + PGA1_c11750 0.19 -2.6 1,216,656 + PGA1_c11750 0.19 -3.3 1,216,656 + PGA1_c11750 0.19 -1.4 1,216,659 - PGA1_c11750 0.19 -1.3 1,216,664 - PGA1_c11750 0.19 -1.1 1,216,664 - PGA1_c11750 0.19 -2.8 1,216,690 - PGA1_c11750 0.20 -2.5 1,216,692 + PGA1_c11750 0.20 -2.4 1,216,736 + PGA1_c11750 0.22 -1.1 1,216,853 + PGA1_c11750 0.25 -2.6 1,216,861 - PGA1_c11750 0.25 -2.2 1,216,913 + PGA1_c11750 0.27 +1.5 1,216,921 + PGA1_c11750 0.27 -1.5 1,216,939 + PGA1_c11750 0.27 -3.3 1,216,958 + PGA1_c11750 0.28 -2.0 1,217,016 + PGA1_c11750 0.30 -1.7 1,217,016 + PGA1_c11750 0.30 -2.4 1,217,016 + PGA1_c11750 0.30 -1.7 1,217,026 + PGA1_c11750 0.30 -3.1 1,217,029 - PGA1_c11750 0.30 -1.8 1,217,029 - PGA1_c11750 0.30 -2.4 1,217,029 - PGA1_c11750 0.30 -1.7 1,217,041 - PGA1_c11750 0.30 -1.1 1,217,229 + PGA1_c11750 0.36 -4.0 1,217,231 - PGA1_c11750 0.36 -1.5 1,217,500 + PGA1_c11750 0.43 -4.1 1,217,611 + PGA1_c11750 0.47 -2.1 1,217,652 + PGA1_c11750 0.48 -3.1 1,217,662 + PGA1_c11750 0.48 -2.9 1,217,715 - PGA1_c11750 0.50 -2.9 1,217,899 - PGA1_c11750 0.55 -3.5 1,217,919 + PGA1_c11750 0.56 -1.7 1,217,957 + PGA1_c11750 0.57 -2.4 1,218,123 + PGA1_c11750 0.61 -3.1 1,218,210 + PGA1_c11750 0.64 -3.1 1,218,338 + PGA1_c11750 0.68 -1.7 1,218,338 + PGA1_c11750 0.68 -3.2 1,218,338 + PGA1_c11750 0.68 -2.6 1,218,346 - PGA1_c11750 0.68 -2.0 1,218,346 - PGA1_c11750 0.68 -1.4 1,218,361 - PGA1_c11750 0.68 -2.6 1,218,361 - PGA1_c11750 0.68 -2.1 1,218,462 + PGA1_c11750 0.71 -0.9 1,218,469 + PGA1_c11750 0.71 -1.5 1,218,470 - PGA1_c11750 0.71 -3.1 1,218,493 + PGA1_c11750 0.72 -1.6 1,218,537 + PGA1_c11750 0.73 -0.5 1,218,669 + PGA1_c11750 0.77 -3.0 1,218,736 + PGA1_c11750 0.79 -3.3 1,218,736 + PGA1_c11750 0.79 -2.5 1,218,945 - PGA1_c11750 0.85 -2.8 1,218,945 - PGA1_c11750 0.85 -4.3 1,218,985 + PGA1_c11750 0.86 -2.1 1,218,985 + PGA1_c11750 0.86 -1.7 1,218,990 + PGA1_c11750 0.86 -0.9 1,219,055 + PGA1_c11750 0.88 -2.0 1,219,055 + PGA1_c11750 0.88 -1.5 1,219,063 - PGA1_c11750 0.88 -0.9 1,219,073 - PGA1_c11750 0.89 -3.2 1,219,076 + PGA1_c11750 0.89 -3.1 1,219,175 + -1.0 1,219,180 + -1.8 1,219,180 + -2.3 1,219,180 + -3.3 1,219,239 + +1.5 1,219,395 + -2.1 1,219,402 + -1.8 1,219,651 + PGA1_c11760 0.11 -1.1 1,219,651 + PGA1_c11760 0.11 -2.7 1,219,665 - PGA1_c11760 0.14 -2.7 1,219,687 - PGA1_c11760 0.18 -3.1 1,219,748 - PGA1_c11760 0.31 -2.4 1,219,803 + PGA1_c11760 0.43 -1.5 1,219,897 + PGA1_c11760 0.63 -1.3 1,219,897 + PGA1_c11760 0.63 -2.5 1,219,989 + PGA1_c11760 0.82 -2.2 1,220,017 + PGA1_c11760 0.88 -2.5 1,220,025 - PGA1_c11760 0.90 -0.5 1,220,060 - -1.1 1,220,070 - -2.1 1,220,133 + -0.4 1,220,133 + +1.3 1,220,151 + -1.0 1,220,151 + +1.3 1,220,151 + -0.7 1,220,151 + -0.5 1,220,159 - +0.3 1,220,159 - +1.1 1,220,210 - PGA1_c11770 0.11 +0.2 1,220,224 - PGA1_c11770 0.13 -0.9 1,220,230 + PGA1_c11770 0.14 -0.9 1,220,290 - PGA1_c11770 0.23 -0.2 1,220,290 - PGA1_c11770 0.23 -0.4 1,220,305 + PGA1_c11770 0.26 -0.6
Or see this region's nucleotide sequence