Experiment: Carbon source L-Citrulline 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c11680 and PGA1_c11690 are separated by 38 nucleotides PGA1_c11690 and PGA1_c11700 are separated by 139 nucleotides PGA1_c11700 and PGA1_c11710 are separated by 96 nucleotides
PGA1_c11680: PGA1_c11680 - acetyltransferase domain-containing protein, at 1,210,494 to 1,211,033
_c11680
PGA1_c11690: PGA1_c11690 - thioesterase domain-containing protein, at 1,211,072 to 1,211,599
_c11690
PGA1_c11700: PGA1_c11700 - 5,10-methylenetetrahydrofolate reductase MetF, at 1,211,739 to 1,212,608
_c11700
PGA1_c11710: PGA1_c11710 - transcriptional regulator, LysR family, at 1,212,705 to 1,213,610
_c11710
Position (kb)
1211
1212
1213 Strain fitness (log2 ratio)
-4
-3
-2
-1
0
1
2 at 1210.755 kb on - strand, within PGA1_c11680 at 1210.887 kb on + strand, within PGA1_c11680 at 1210.887 kb on + strand, within PGA1_c11680 at 1210.900 kb on - strand, within PGA1_c11680 at 1210.951 kb on + strand, within PGA1_c11680 at 1210.968 kb on - strand, within PGA1_c11680 at 1210.996 kb on - strand at 1211.027 kb on + strand at 1211.032 kb on + strand at 1211.065 kb on + strand at 1211.073 kb on - strand at 1211.073 kb on - strand at 1211.073 kb on - strand at 1211.089 kb on + strand at 1211.229 kb on - strand, within PGA1_c11690 at 1211.261 kb on + strand, within PGA1_c11690 at 1211.269 kb on - strand, within PGA1_c11690 at 1211.333 kb on - strand, within PGA1_c11690 at 1211.339 kb on + strand, within PGA1_c11690 at 1211.347 kb on - strand, within PGA1_c11690 at 1211.366 kb on - strand, within PGA1_c11690 at 1211.407 kb on + strand, within PGA1_c11690 at 1211.407 kb on + strand, within PGA1_c11690 at 1211.415 kb on + strand, within PGA1_c11690 at 1211.415 kb on + strand, within PGA1_c11690 at 1211.417 kb on + strand, within PGA1_c11690 at 1211.425 kb on - strand, within PGA1_c11690 at 1211.430 kb on - strand, within PGA1_c11690 at 1211.430 kb on - strand, within PGA1_c11690 at 1211.442 kb on + strand, within PGA1_c11690 at 1211.443 kb on - strand, within PGA1_c11690 at 1211.533 kb on + strand, within PGA1_c11690 at 1211.569 kb on + strand at 1211.569 kb on + strand at 1211.573 kb on - strand at 1211.577 kb on - strand at 1211.577 kb on - strand at 1211.577 kb on - strand at 1211.578 kb on - strand at 1211.589 kb on + strand at 1211.628 kb on - strand at 1211.709 kb on - strand at 1211.799 kb on + strand at 1211.804 kb on + strand at 1211.807 kb on - strand at 1211.812 kb on - strand at 1211.812 kb on - strand at 1211.812 kb on - strand at 1211.829 kb on - strand, within PGA1_c11700 at 1211.838 kb on + strand, within PGA1_c11700 at 1211.858 kb on + strand, within PGA1_c11700 at 1211.866 kb on - strand, within PGA1_c11700 at 1211.866 kb on - strand, within PGA1_c11700 at 1211.883 kb on - strand, within PGA1_c11700 at 1211.959 kb on - strand, within PGA1_c11700 at 1211.989 kb on + strand, within PGA1_c11700 at 1212.048 kb on - strand, within PGA1_c11700 at 1212.131 kb on - strand, within PGA1_c11700 at 1212.147 kb on + strand, within PGA1_c11700 at 1212.147 kb on + strand, within PGA1_c11700 at 1212.147 kb on + strand, within PGA1_c11700 at 1212.147 kb on + strand, within PGA1_c11700 at 1212.155 kb on - strand, within PGA1_c11700 at 1212.155 kb on - strand, within PGA1_c11700 at 1212.155 kb on - strand, within PGA1_c11700 at 1212.181 kb on - strand, within PGA1_c11700 at 1212.273 kb on - strand, within PGA1_c11700 at 1212.361 kb on - strand, within PGA1_c11700 at 1212.396 kb on + strand, within PGA1_c11700 at 1212.396 kb on + strand, within PGA1_c11700 at 1212.396 kb on + strand, within PGA1_c11700 at 1212.396 kb on + strand, within PGA1_c11700 at 1212.396 kb on + strand, within PGA1_c11700 at 1212.396 kb on + strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.404 kb on - strand, within PGA1_c11700 at 1212.437 kb on - strand, within PGA1_c11700 at 1212.525 kb on + strand at 1212.525 kb on + strand at 1212.525 kb on + strand at 1212.525 kb on + strand at 1212.533 kb on - strand at 1212.533 kb on - strand at 1212.602 kb on - strand at 1212.636 kb on - strand at 1212.687 kb on + strand at 1212.782 kb on + strand at 1212.790 kb on - strand at 1212.846 kb on + strand, within PGA1_c11710 at 1212.932 kb on + strand, within PGA1_c11710 at 1212.932 kb on + strand, within PGA1_c11710 at 1212.932 kb on + strand, within PGA1_c11710 at 1212.940 kb on - strand, within PGA1_c11710 at 1212.940 kb on - strand, within PGA1_c11710 at 1213.043 kb on - strand, within PGA1_c11710 at 1213.050 kb on + strand, within PGA1_c11710 at 1213.068 kb on - strand, within PGA1_c11710 at 1213.097 kb on - strand, within PGA1_c11710 at 1213.118 kb on + strand, within PGA1_c11710 at 1213.204 kb on - strand, within PGA1_c11710 at 1213.273 kb on + strand, within PGA1_c11710 at 1213.281 kb on + strand, within PGA1_c11710 at 1213.281 kb on - strand, within PGA1_c11710 at 1213.346 kb on + strand, within PGA1_c11710 at 1213.354 kb on - strand, within PGA1_c11710 at 1213.433 kb on + strand, within PGA1_c11710 at 1213.438 kb on + strand, within PGA1_c11710 at 1213.479 kb on - strand, within PGA1_c11710 at 1213.497 kb on + strand, within PGA1_c11710 at 1213.497 kb on - strand, within PGA1_c11710 at 1213.497 kb on - strand, within PGA1_c11710 at 1213.510 kb on + strand, within PGA1_c11710 at 1213.573 kb on - strand at 1213.574 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source L-Citrulline 5 mM remove 1,210,755 - PGA1_c11680 0.48 -0.8 1,210,887 + PGA1_c11680 0.73 -0.1 1,210,887 + PGA1_c11680 0.73 -0.8 1,210,900 - PGA1_c11680 0.75 -0.0 1,210,951 + PGA1_c11680 0.85 -0.6 1,210,968 - PGA1_c11680 0.88 +0.5 1,210,996 - +0.4 1,211,027 + -0.6 1,211,032 + -0.0 1,211,065 + +1.1 1,211,073 - -0.1 1,211,073 - +0.5 1,211,073 - +0.6 1,211,089 + -0.4 1,211,229 - PGA1_c11690 0.30 +0.2 1,211,261 + PGA1_c11690 0.36 -0.3 1,211,269 - PGA1_c11690 0.37 +0.2 1,211,333 - PGA1_c11690 0.49 -1.9 1,211,339 + PGA1_c11690 0.51 +0.0 1,211,347 - PGA1_c11690 0.52 +0.3 1,211,366 - PGA1_c11690 0.56 -0.1 1,211,407 + PGA1_c11690 0.63 -0.4 1,211,407 + PGA1_c11690 0.63 -1.2 1,211,415 + PGA1_c11690 0.65 +0.7 1,211,415 + PGA1_c11690 0.65 -2.4 1,211,417 + PGA1_c11690 0.65 +1.1 1,211,425 - PGA1_c11690 0.67 +0.4 1,211,430 - PGA1_c11690 0.68 +2.1 1,211,430 - PGA1_c11690 0.68 -0.2 1,211,442 + PGA1_c11690 0.70 -1.3 1,211,443 - PGA1_c11690 0.70 -0.2 1,211,533 + PGA1_c11690 0.87 -0.9 1,211,569 + -0.1 1,211,569 + +1.5 1,211,573 - +2.1 1,211,577 - +0.8 1,211,577 - -0.1 1,211,577 - +0.6 1,211,578 - -0.3 1,211,589 + -0.3 1,211,628 - +0.8 1,211,709 - +0.4 1,211,799 + -0.9 1,211,804 + -2.7 1,211,807 - +0.2 1,211,812 - -3.1 1,211,812 - -1.9 1,211,812 - -0.6 1,211,829 - PGA1_c11700 0.10 -3.1 1,211,838 + PGA1_c11700 0.11 -1.9 1,211,858 + PGA1_c11700 0.14 -2.9 1,211,866 - PGA1_c11700 0.15 -2.3 1,211,866 - PGA1_c11700 0.15 -2.1 1,211,883 - PGA1_c11700 0.17 -4.3 1,211,959 - PGA1_c11700 0.25 -4.3 1,211,989 + PGA1_c11700 0.29 -1.6 1,212,048 - PGA1_c11700 0.36 -0.3 1,212,131 - PGA1_c11700 0.45 -1.2 1,212,147 + PGA1_c11700 0.47 -3.5 1,212,147 + PGA1_c11700 0.47 -3.7 1,212,147 + PGA1_c11700 0.47 -1.9 1,212,147 + PGA1_c11700 0.47 -2.2 1,212,155 - PGA1_c11700 0.48 -2.6 1,212,155 - PGA1_c11700 0.48 -1.5 1,212,155 - PGA1_c11700 0.48 -3.0 1,212,181 - PGA1_c11700 0.51 -2.0 1,212,273 - PGA1_c11700 0.61 -3.1 1,212,361 - PGA1_c11700 0.71 -1.1 1,212,396 + PGA1_c11700 0.76 -1.4 1,212,396 + PGA1_c11700 0.76 -3.6 1,212,396 + PGA1_c11700 0.76 -2.7 1,212,396 + PGA1_c11700 0.76 -3.3 1,212,396 + PGA1_c11700 0.76 -1.7 1,212,396 + PGA1_c11700 0.76 -2.7 1,212,404 - PGA1_c11700 0.76 -1.7 1,212,404 - PGA1_c11700 0.76 -4.1 1,212,404 - PGA1_c11700 0.76 -0.6 1,212,404 - PGA1_c11700 0.76 -4.3 1,212,404 - PGA1_c11700 0.76 -1.5 1,212,404 - PGA1_c11700 0.76 -2.2 1,212,404 - PGA1_c11700 0.76 -2.0 1,212,404 - PGA1_c11700 0.76 -3.3 1,212,437 - PGA1_c11700 0.80 -2.8 1,212,525 + -4.7 1,212,525 + -2.0 1,212,525 + -3.7 1,212,525 + -3.3 1,212,533 - -1.4 1,212,533 - -0.9 1,212,602 - -1.6 1,212,636 - -1.3 1,212,687 + -2.6 1,212,782 + -2.2 1,212,790 - -3.7 1,212,846 + PGA1_c11710 0.16 -0.9 1,212,932 + PGA1_c11710 0.25 -0.9 1,212,932 + PGA1_c11710 0.25 -3.3 1,212,932 + PGA1_c11710 0.25 -1.5 1,212,940 - PGA1_c11710 0.26 -2.0 1,212,940 - PGA1_c11710 0.26 -1.7 1,213,043 - PGA1_c11710 0.37 -1.6 1,213,050 + PGA1_c11710 0.38 -2.0 1,213,068 - PGA1_c11710 0.40 -2.3 1,213,097 - PGA1_c11710 0.43 -1.1 1,213,118 + PGA1_c11710 0.46 -1.9 1,213,204 - PGA1_c11710 0.55 -1.1 1,213,273 + PGA1_c11710 0.63 -2.0 1,213,281 + PGA1_c11710 0.64 -1.8 1,213,281 - PGA1_c11710 0.64 -2.9 1,213,346 + PGA1_c11710 0.71 -2.2 1,213,354 - PGA1_c11710 0.72 -3.3 1,213,433 + PGA1_c11710 0.80 -1.4 1,213,438 + PGA1_c11710 0.81 -2.2 1,213,479 - PGA1_c11710 0.85 -1.9 1,213,497 + PGA1_c11710 0.87 -1.6 1,213,497 - PGA1_c11710 0.87 -2.5 1,213,497 - PGA1_c11710 0.87 -3.4 1,213,510 + PGA1_c11710 0.89 -2.7 1,213,573 - -1.9 1,213,574 + +0.9
Or see this region's nucleotide sequence