Experiment: Carbon source L-Ornithine 5 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt PGA1_c00020 and PGA1_c00030 are separated by 97 nucleotides PGA1_c00030 and PGA1_c00040 overlap by 4 nucleotides PGA1_c00040 and PGA1_c00050 are separated by 14 nucleotides
PGA1_c00020: PGA1_c00020 - DNA polymerase III subunit beta, at 1,838 to 2,959
_c00020
PGA1_c00030: PGA1_c00030 - DNA replication and repair protein RecF, at 3,057 to 4,154
_c00030
PGA1_c00040: PGA1_c00040 - putative LysE type translocator, at 4,151 to 4,771
_c00040
PGA1_c00050: PGA1_c00050 - putative transcriptional regulator, tetR family, at 4,786 to 5,385
_c00050
Position (kb)
3
4
5 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2.953 kb on + strand at 2.953 kb on + strand at 2.953 kb on + strand at 2.961 kb on - strand at 2.963 kb on + strand at 2.969 kb on + strand at 2.990 kb on + strand at 2.990 kb on + strand at 2.998 kb on - strand at 2.998 kb on - strand at 3.053 kb on + strand at 3.070 kb on - strand at 3.073 kb on + strand at 3.073 kb on + strand at 3.093 kb on + strand at 3.131 kb on + strand at 3.248 kb on + strand, within PGA1_c00030 at 3.281 kb on + strand, within PGA1_c00030 at 3.289 kb on + strand, within PGA1_c00030 at 3.317 kb on - strand, within PGA1_c00030 at 3.342 kb on - strand, within PGA1_c00030 at 3.386 kb on - strand, within PGA1_c00030 at 3.510 kb on + strand, within PGA1_c00030 at 3.584 kb on - strand, within PGA1_c00030 at 3.640 kb on + strand, within PGA1_c00030 at 3.674 kb on - strand, within PGA1_c00030 at 3.707 kb on - strand, within PGA1_c00030 at 3.732 kb on + strand, within PGA1_c00030 at 3.750 kb on - strand, within PGA1_c00030 at 3.763 kb on + strand, within PGA1_c00030 at 3.799 kb on + strand, within PGA1_c00030 at 3.807 kb on - strand, within PGA1_c00030 at 3.807 kb on - strand, within PGA1_c00030 at 3.831 kb on - strand, within PGA1_c00030 at 3.848 kb on + strand, within PGA1_c00030 at 3.848 kb on + strand, within PGA1_c00030 at 3.897 kb on + strand, within PGA1_c00030 at 4.009 kb on - strand, within PGA1_c00030 at 4.035 kb on - strand, within PGA1_c00030 at 4.046 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.066 kb on + strand at 4.074 kb on - strand at 4.074 kb on - strand at 4.074 kb on - strand at 4.098 kb on + strand at 4.103 kb on + strand at 4.111 kb on + strand at 4.114 kb on - strand at 4.132 kb on + strand at 4.195 kb on - strand at 4.212 kb on - strand at 4.235 kb on - strand, within PGA1_c00040 at 4.290 kb on - strand, within PGA1_c00040 at 4.369 kb on + strand, within PGA1_c00040 at 4.402 kb on - strand, within PGA1_c00040 at 4.480 kb on + strand, within PGA1_c00040 at 4.522 kb on - strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.580 kb on + strand, within PGA1_c00040 at 4.581 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.656 kb on - strand, within PGA1_c00040 at 4.666 kb on - strand, within PGA1_c00040 at 4.671 kb on - strand, within PGA1_c00040 at 4.777 kb on + strand at 4.780 kb on - strand at 4.780 kb on - strand at 4.785 kb on + strand at 4.825 kb on + strand at 4.858 kb on + strand, within PGA1_c00050 at 4.873 kb on + strand, within PGA1_c00050 at 4.913 kb on + strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.921 kb on - strand, within PGA1_c00050 at 4.950 kb on + strand, within PGA1_c00050 at 4.971 kb on + strand, within PGA1_c00050 at 4.979 kb on - strand, within PGA1_c00050 at 5.031 kb on - strand, within PGA1_c00050 at 5.057 kb on - strand, within PGA1_c00050 at 5.068 kb on - strand, within PGA1_c00050 at 5.103 kb on + strand, within PGA1_c00050 at 5.148 kb on - strand, within PGA1_c00050 at 5.152 kb on + strand, within PGA1_c00050
Per-strain Table
Position Strand Gene LocusTag Fraction Carbon source L-Ornithine 5 mM remove 2,953 + -0.1 2,953 + +1.1 2,953 + +0.1 2,961 - -0.6 2,963 + +0.9 2,969 + -0.2 2,990 + +1.2 2,990 + -0.8 2,998 - -0.2 2,998 - +0.4 3,053 + -2.1 3,070 - +0.1 3,073 + +0.1 3,073 + -2.8 3,093 + -1.1 3,131 + +1.5 3,248 + PGA1_c00030 0.17 -1.5 3,281 + PGA1_c00030 0.20 +0.5 3,289 + PGA1_c00030 0.21 +1.4 3,317 - PGA1_c00030 0.24 -0.2 3,342 - PGA1_c00030 0.26 +0.9 3,386 - PGA1_c00030 0.30 -0.7 3,510 + PGA1_c00030 0.41 +0.4 3,584 - PGA1_c00030 0.48 -0.9 3,640 + PGA1_c00030 0.53 -1.6 3,674 - PGA1_c00030 0.56 -0.2 3,707 - PGA1_c00030 0.59 -1.3 3,732 + PGA1_c00030 0.61 -0.3 3,750 - PGA1_c00030 0.63 +0.8 3,763 + PGA1_c00030 0.64 -2.4 3,799 + PGA1_c00030 0.68 -1.1 3,807 - PGA1_c00030 0.68 +1.9 3,807 - PGA1_c00030 0.68 +0.2 3,831 - PGA1_c00030 0.70 +0.5 3,848 + PGA1_c00030 0.72 +0.4 3,848 + PGA1_c00030 0.72 +1.1 3,897 + PGA1_c00030 0.77 +2.7 4,009 - PGA1_c00030 0.87 +2.1 4,035 - PGA1_c00030 0.89 +0.4 4,046 + -1.7 4,066 + -0.7 4,066 + +1.4 4,066 + +1.7 4,066 + -0.9 4,074 - +0.3 4,074 - -0.9 4,074 - +0.3 4,098 + +0.3 4,103 + +0.2 4,111 + -1.0 4,114 - +0.6 4,132 + -0.1 4,195 - -0.2 4,212 - +0.3 4,235 - PGA1_c00040 0.14 -0.7 4,290 - PGA1_c00040 0.22 -0.7 4,369 + PGA1_c00040 0.35 -0.1 4,402 - PGA1_c00040 0.40 +0.5 4,480 + PGA1_c00040 0.53 -0.2 4,522 - PGA1_c00040 0.60 +0.2 4,580 + PGA1_c00040 0.69 -0.3 4,580 + PGA1_c00040 0.69 -1.3 4,581 - PGA1_c00040 0.69 -0.2 4,656 - PGA1_c00040 0.81 -0.2 4,656 - PGA1_c00040 0.81 -0.2 4,666 - PGA1_c00040 0.83 +1.2 4,671 - PGA1_c00040 0.84 -0.2 4,777 + -1.5 4,780 - -0.5 4,780 - +0.3 4,785 + +0.8 4,825 + +2.1 4,858 + PGA1_c00050 0.12 -0.2 4,873 + PGA1_c00050 0.14 +1.1 4,913 + PGA1_c00050 0.21 +2.1 4,921 - PGA1_c00050 0.23 +0.1 4,921 - PGA1_c00050 0.23 +0.1 4,950 + PGA1_c00050 0.27 +0.5 4,971 + PGA1_c00050 0.31 +1.4 4,979 - PGA1_c00050 0.32 +1.1 5,031 - PGA1_c00050 0.41 +0.5 5,057 - PGA1_c00050 0.45 -1.2 5,068 - PGA1_c00050 0.47 +0.5 5,103 + PGA1_c00050 0.53 +1.1 5,148 - PGA1_c00050 0.60 +0.1 5,152 + PGA1_c00050 0.61 +0.5
Or see this region's nucleotide sequence